Restriction map for
pFN21AB9759
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# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AhdI Alw44I AocI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI BamHI BcgI BciVI
BfrI BglII BsaBI BseAI BsiWI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrGI Bst1107I BstBI BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
CpoI DraI Eam1105I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI HindIII
HinfI HpaI KpnI MamI MfeI
MroI MseI MunI NcoI NdeI
NheI NruI NsiI NspV PacI
PleI PmeI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SalI
SbfI SexAI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tsp509I XbaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AgeI 1150 ACCGGT A^CCGGT
AlwI 354 GATCC GGATC(4/5)
Asp700I 1566 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1681 GAGCAC G[AT]GC[AT]^C
AspI 1414 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1386 CCTAGG C^CTAGG
BbsI 1521 GAAGAC GAAGAC(2/6)
BbvCI 2049 CCTCAGC CCTCAGC(-5/-2)
BclI 89 TGATCA T^GATCA
BlnI 1386 CCTAGG C^CTAGG
BpuAI 1521 GAAGAC GAAGAC(2/6)
BsaI 1147 GAGACC GGTCTC(1/5)
BsaWI 1150 ACCGGT [AT]^CCGG[AT]
BsiHKAI 1681 GAGCAC G[AT]GC[AT]^C
BsmBI 1035 CGTCTC CGTCTC(1/5)
BsmI 1259 GCATTC GAATGC(1/-1)
BssHII 119 GCGCGC G^CGCGC
BstYI 353 AGATCC [GA]^GATC[CT]
DrdI 1027 GACGCGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1396 CCTTTTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 89 TGATCA T^GATCA
HincII 574 GTCAAC GT[CT]^[GA]AC
HindII 574 GTCAAC GT[CT]^[GA]AC
MaeIII 1241 GTGAC ^GT[ACGT]AC
MflI 353 AGATCC [GA]^GATC[CT]
PaeR7I 1189 CTCGAG C^TCGAG
PflFI 1414 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1150 ACCGGT A^CCGGT
ScaI 404 AGTACT AGT^ACT
SphI 1649 GCATGC GCATG^C
StuI 500 AGGCCT AGG^CCT
Tsp45I 1241 GTGAC ^GT[GC]AC
Tth111I 1414 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 1994 CCAAGGTGGCGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1189 CTCGAG C^TCGAG
XhoII 353 AGATCC [GA]^GATC[CT]
XmnI 1566 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwNI 37 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
826 CAGAAGCTG
Aor51HI 1004 AGCGCT AGC^GCT
1335 AGCGCT
AviII 1604 TGCGCA TGC^GCA
1688 TGCGCA
BalI 1182 TGGCCA TGG^CCA
2024 TGGCCA
BbrPI 1306 CACGTG CAC^GTG
1318 CACGTG
BfaI 917 CTAG C^TAG
1387 CTAG
BsgI 1984 CTGCAC GTGCAG(16/14)
100 GTGCAG
BsrBI 1054 CCGCTC CCGCTC(-3/-3)
709 GAGCGG
DpnII 90 GATC ^GATC
354 GATC
DraIII 626 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
1780 CACGCCGTG
Eco47III 1004 AGCGCT AGC^GCT
1335 AGCGCT
FspI 1604 TGCGCA TGC^GCA
1688 TGCGCA
MaeI 917 CTAG C^TAG
1387 CTAG
MboI 90 GATC ^GATC
354 GATC
MluI 599 ACGCGT A^CGCGT
1640 ACGCGT
MluNI 1182 TGGCCA TGG^CCA
2024 TGGCCA
MscI 1182 TGGCCA TGG^CCA
2024 TGGCCA
MslI 93 CATCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1770 CATGAAGATG
NdeII 90 GATC ^GATC
354 GATC
PflMI 198 CCATGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
825 CCAGAAGCTGG
PmaCI 1306 CACGTG CAC^GTG
1318 CACGTG
PmlI 1306 CACGTG CAC^GTG
1318 CACGTG
PstI 46 CTGCAG CTGCA^G
349 CTGCAG
SapI 681 GAAGAGC GCTCTTC(1/4)
1012 GAAGAGC
Sau3AI 90 GATC ^GATC
354 GATC
SfaNI 1776 GATGC GCATC(5/9)
1691 GCATC
SfcI 46 CTGCAG C^T[GA][CT]AG
349 CTGCAG
TspRI 583 CACTG CA[GC]TG[ACGT][ACGT]^
1528 CACTG
Van91I 198 CCATGACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
825 CCAGAAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI ApaI AvaI AvaII BanI
BanII BbeI BbvI BcnI BglI
BlpI BmyI BpmI Bpu1102I BsaAI
BsaHI BsaJI BseRI BsiEI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI Cac8I
CelII CfoI Cfr10I Cfr13I DdeI
DraII DsaI EaeI EagI EarI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HpaII HphI ItaI KasI Ksp632I
KspI MaeII MboII MnlI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NgoMIV NlaIII NlaIV
NotI NspI PpuMI PvuII RsaI
SacII Sau96I ScrFI SfiI SfoI
SmaI SmlI StyI TaqI TseI
TthHB8I XmaCI XmaI