Restriction map for
pFN21AB7280
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# NO CUT ENZYMES ( 64 enzymes) #
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AclI AcsI AgeI ApoI AscI
AseI AsnI AvrII BbvCI BciVI
BclI BfaI BglII BlnI BsaBI
BsiWI BspDI BspLU11I BssHII Bst1107I
BstAPI BstBI BstXI BstZ17I ClaI
CpoI DraI DraIII EcoRI EcoRV
EcoT22I FbaI HindIII HpaI MaeI
MamI MfeI MluI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PflMI PinAI PmeI PshBI
Psp1406I PvuI RsrII SalI ScaI
SfuI SgrAI SnaBI SpeI SspI
SwaI Tsp509I Van91I XbaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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Acc65I 242 GGTACC G^GTACC
AccI 2137 GTCTAC GT^[AC][GT]AC
AflII 1918 CTTAAG C^TTAAG
AflIII 2141 ACGTGT A^C[GA][CT]GT
AhdI 2129 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1879 AGCGCT AGC^GCT
Asp718I 242 GGTACC G^GTACC
AspEI 2129 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2083 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 674 TGCGCA TGC^GCA
BbsI 1234 GAAGAC GAAGAC(2/6)
BfrI 1918 CTTAAG C^TTAAG
BlpI 661 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 661 GCTGAGC GC^T[ACGT]AGC
BpuAI 1234 GAAGAC GAAGAC(2/6)
Bsp1407I 3629 TGTACA T^GTACA
BspHI 3992 TCATGA T^CATGA
BsrGI 3629 TGTACA T^GTACA
BstEII 165 GGTCACC G^GT[ACGT]ACC
BstPI 165 GGTCACC G^GT[ACGT]ACC
CelII 661 GCTGAGC GC^T[ACGT]AGC
Eam1105I 2129 GACCTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1879 AGCGCT AGC^GCT
EcoNI 708 CCTGGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 165 GGTCACC G^GT[ACGT]ACC
FseI 2188 GGCCGGCC GGCCGG^CC
FspI 674 TGCGCA TGC^GCA
HgaI 769 GACGC GACGC(5/10)
KpnI 242 GGTACC GGTAC^C
MseI 1919 TTAA T^TAA
NspI 3524 GCATGC [GA]CATG^[CT]
PaeR7I 1457 CTCGAG C^TCGAG
PflFI 2083 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1288 GACGTAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3992 TCATGA T^CATGA
SphI 3524 GCATGC GCATG^C
SspBI 3629 TGTACA T^GTACA
Tru9I 1919 TTAA T^TAA
Tth111I 2083 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 1457 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 20 enzymes) #
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Alw44I 3118 GTGCAC G^TGCAC
3421 GTGCAC
ApaLI 3118 GTGCAC G^TGCAC
3421 GTGCAC
Asp700I 3186 GAACGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3984 GAACAGCTTC
BbrPI 960 CACGTG CAC^GTG
2837 CACGTG
BcgI 2313 CGACGGCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
755 GCAGCGGGCTCG
BsmBI 793 GAGACG CGTCTC(1/5)
3865 GAGACG
BsmI 554 GAATGC GAATGC(1/-1)
2510 GAATGC
BsrBI 629 GAGCGG CCGCTC(-3/-3)
1743 GAGCGG
BsrDI 2016 CATTGC GCAATG(2/0)
2573 GCAATG
HincII 1866 GTCAAC GT[CT]^[GA]AC
3673 GTCAAC
HindII 1866 GTCAAC GT[CT]^[GA]AC
3673 GTCAAC
MslI 1404 CATTGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3144 CATCTTCGTG
PmaCI 960 CACGTG CAC^GTG
2837 CACGTG
PmlI 960 CACGTG CAC^GTG
2837 CACGTG
SacI 926 GAGCTC GAGCT^C
3166 GAGCTC
SapI 3807 GAAGAGC GCTCTTC(1/4)
606 GCTCTTC
StuI 1497 AGGCCT AGG^CCT
3902 AGGCCT
TaqI 896 TCGA T^CGA
1458 TCGA
TthHB8I 896 TCGA T^CGA
1458 TCGA
XmnI 3186 GAACGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3984 GAACAGCTTC
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# 3 OR MORE CUT ENZYMES ( 134 enzymes) #
#########################################
AatII AccII AccIII AciI AcyI
AfaI AluI AlwI AlwNI AocI
ApaI AspHI AvaI AvaII BalI
BamHI BanI BanII BbeI BbvI
BcnI BglI BmyI BpmI BsaAI
BsaHI BsaI BsaJI BsaWI BseAI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspEI BspMI BsrFI BsrI BssKI
BssSI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DrdI DsaI
EaeI EagI EarI EclXI Eco52I
Eco81I EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I KspI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MroI MscI MspA1I
MspI MvaI MvnI MwoI NaeI
NarI NciI NcoI NdeII NgoMIV
NlaIII NlaIV PleI PpuMI PstI
PvuII RsaI SacII Sau3AI Sau96I
SbfI ScrFI SexAI SfaNI SfcI
SfiI SfoI SmaI SmlI Sse8387I
StyI TfiI TseI Tsp45I TspRI
XcmI XhoII XmaCI XmaI