Restriction map for
pFN21AB8375
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# NO CUT ENZYMES ( 99 enzymes) #
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Acc65I AccI AccIII AclI AflII
AgeI AhdI Alw44I ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BalI BbeI
BciVI BfrI BlnI BsaBI BseAI
BsiWI Bsp1407I BspEI BspHI BsrDI
BsrGI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I CpoI DraI DraIII
Eam1105I EarI EcoNI EcoO65I EcoRV
EcoT22I FseI FspI HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MluNI MroI MscI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 682 GACGTC GACGT^C
AcsI 235 GAATTC [GA]^AATT[CT]
AcyI 682 GACGTC G[GA]^CG[CT]C
AflIII 253 ACATGT A^C[GA][CT]GT
AlwNI 1024 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 407 CCTCAGG CC^T[ACGT]AGG
ApaI 124 GGGCCC GGGCC^C
ApoI 235 GAATTC [GA]^AATT[CT]
BamHI 1280 GGATCC G^GATCC
BbrPI 146 CACGTG CAC^GTG
BbvCI 1010 CCTCAGC CCTCAGC(-5/-2)
BcgI 174 CGAGGGGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 263 TGATCA T^GATCA
BglI 1783 GCCCAGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 682 GACGTC G[GA]^CG[CT]C
BsaWI 1518 TCCGGT [AT]^CCGG[AT]
BspDI 688 ATCGAT AT^CGAT
BspLU11I 253 ACATGT A^CATGT
BspMI 772 ACCTGC ACCTGC(4/8)
BsrFI 988 ACCGGC [GA]^CCGG[CT]
BstAPI 544 GCAAACGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 407 CCTCAGG CC^T[ACGT]AGG
Cfr10I 988 ACCGGC [GA]^CCGG[CT]
ClaI 688 ATCGAT AT^CGAT
DrdI 1417 GACTTCCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 407 CCTCAGG CC^T[ACGT]AGG
EcoRI 235 GAATTC G^AATTC
FbaI 263 TGATCA T^GATCA
Hin1I 682 GACGTC G[GA]^CG[CT]C
HincII 1841 GTCAAC GT[CT]^[GA]AC
HindII 1841 GTCAAC GT[CT]^[GA]AC
MseI 1826 TTAA T^TAA
NcoI 836 CCATGG C^CATGG
PmaCI 146 CACGTG CAC^GTG
PmlI 146 CACGTG CAC^GTG
PshAI 162 GACGACAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1312 GAGCTC GAGCT^C
SexAI 611 ACCTGGT A^CC[AT]GGT
Tru9I 1826 TTAA T^TAA
Tsp509I 236 AATT ^AATT
XbaI 55 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccII 904 CGCG CG^CG
1846 CGCG
AspHI 49 GTGCTC G[AT]GC[AT]^C
1312 GAGCTC
BbsI 1458 GAAGAC GAAGAC(2/6)
982 GTCTTC
BglII 443 AGATCT A^GATCT
532 AGATCT
BpmI 83 CTCCAG CTGGAG(16/14)
1234 CTGGAG
BpuAI 1458 GAAGAC GAAGAC(2/6)
982 GTCTTC
BsaAI 146 CACGTG [CT]AC^GT[GA]
961 TACGTG
BsiHKAI 49 GTGCTC G[AT]GC[AT]^C
1312 GAGCTC
BsmI 281 GCATTC GAATGC(1/-1)
1562 GCATTC
BsrBI 36 CCGCTC CCGCTC(-3/-3)
1276 GAGCGG
BssSI 862 CACGAG CACGAG(-5/-1)
1688 CTCGTG
BstUI 904 CGCG CG^CG
1846 CGCG
BstXI 716 CCACCTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1173 CCAGCTTGGTGG
DsaI 434 CCGTGG C^C[GA][CT]GG
836 CCATGG
EcoT14I 836 CCATGG C^C[AT][AT]GG
998 CCAAGG
MslI 777 CACAGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
783 CATGGCGGTG
MvnI 904 CGCG CG^CG
1846 CGCG
NspI 253 ACATGT [GA]CATG^[CT]
462 GCATGT
PleI 1640 GACTC GAGTC(4/5)
1091 GAGTC
PvuII 731 CAGCTG CAG^CTG
1348 CAGCTG
SmaI 44 CCCGGG CCC^GGG
560 CCCGGG
StyI 836 CCATGG C^C[AT][AT]GG
998 CCAAGG
TfiI 302 GAATC G^A[AT]TC
878 GAATC
XmaCI 44 CCCGGG C^CCGGG
560 CCCGGG
XmaI 44 CCCGGG C^CCGGG
560 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AfaI AluI AlwI Aor51HI
AvaI AvaII BanI BanII BbvI
BcnI BfaI BlpI BmyI Bpu1102I
BsaI BsaJI BseRI BsgI BsiEI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII EaeI
EagI EclXI Eco47III Eco52I EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PflMI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
TaqI TseI Tsp45I TspRI TthHB8I
Van91I XcmI XhoII