Restriction map for
pFN21AB8522
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# NO CUT ENZYMES ( 103 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AlwNI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AvrII BbvCI
BclI BfrI BlnI BlpI Bpu1102I
BsaI BsaWI BseAI BseRI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BsrFI BssSI BstBI CelII Cfr10I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I EcoRI EcoRV
FbaI FseI HpaI KpnI KspI
MfeI MluI MroI MspA1I MunI
NaeI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccI 2148 GTATAC GT^[AC][GT]AC
AocI 1696 CCTGAGG CC^T[ACGT]AGG
Asp700I 1427 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 847 GAGCTC G[AT]GC[AT]^C
AvaI 1936 CTCGGG C^[CT]CG[GA]G
BamHI 388 GGATCC G^GATCC
BanII 847 GAGCTC G[GA]GC[CT]^C
BbeI 955 GGCGCC GGCGC^C
BbrPI 2331 CACGTG CAC^GTG
BfaI 1474 CTAG C^TAG
BglI 2423 GCCTGAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 608 AGATCT A^GATCT
BsaBI 1587 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 999 CGACCG CG[GA][CT]^CG
BsiHKAI 847 GAGCTC G[AT]GC[AT]^C
BsmAI 670 GAGAC GTCTC(1/5)
BsoBI 1936 CTCGGG C^[CT]CG[GA]G
BspLU11I 292 ACATGT A^CATGT
BspMI 476 ACCTGC ACCTGC(4/8)
BsrBI 744 CCGCTC CCGCTC(-3/-3)
Bst1107I 2148 GTATAC GTA^TAC
BstEII 109 GGTGACC G^GT[ACGT]ACC
BstPI 109 GGTGACC G^GT[ACGT]ACC
BstZ17I 2148 GTATAC GTA^TAC
Bsu36I 1696 CCTGAGG CC^T[ACGT]AGG
DraIII 2328 CACCACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 630 GACCTACGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1696 CCTGAGG CC^T[ACGT]AGG
EcoNI 1692 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 109 GGTGACC G^GT[ACGT]ACC
HindIII 1249 AAGCTT A^AGCTT
KasI 955 GGCGCC G^GCGCC
MaeI 1474 CTAG C^TAG
MamI 1587 GATTTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 955 GGCGCC GG^CGCC
PmaCI 2331 CACGTG CAC^GTG
PmlI 2331 CACGTG CAC^GTG
PstI 1667 CTGCAG CTGCA^G
SacI 847 GAGCTC GAGCT^C
SapI 486 GCTCTTC GCTCTTC(1/4)
SfoI 955 GGCGCC GGC^GCC
StuI 683 AGGCCT AGG^CCT
XmnI 1427 GAAAGTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AcsI 2041 AAATTC [GA]^AATT[CT]
2289 AAATTT
AcyI 955 GGCGCC G[GA]^CG[CT]C
1720 GACGCC
ApoI 2041 AAATTC [GA]^AATT[CT]
2289 AAATTT
AviII 707 TGCGCA TGC^GCA
827 TGCGCA
BalI 893 TGGCCA TGG^CCA
1130 TGGCCA
BanI 955 GGCGCC G^G[CT][GA]CC
2137 GGCACC
BbsI 184 GAAGAC GAAGAC(2/6)
1717 GAAGAC
BcgI 1530 CGACCCCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1834 CGAGAACTCTGC
BciVI 288 GGATAC GTATCC(6/5)
303 GGATAC
BcnI 1053 CCGGG CC^[GC]GG
1499 CCCGG
BpuAI 184 GAAGAC GAAGAC(2/6)
1717 GAAGAC
BsaHI 955 GGCGCC G[GA]^CG[CT]C
1720 GACGCC
BssHII 805 GCGCGC G^CGCGC
911 GCGCGC
BstAPI 710 GCAACATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1212 GCACAAAATGC
DraII 125 GGGGCCT [GA]G^G[ACGT]CC[CT]
2129 AGGCCCC
DsaI 599 CCATGG C^C[GA][CT]GG
1196 CCATGG
EaeI 893 TGGCCA [CT]^GGCC[GA]
1130 TGGCCA
EarI 487 CTCTTC CTCTTC(1/4)
820 CTCTTC
EcoO109I 125 GGGGCCT [GA]G^G[ACGT]CC[CT]
2129 AGGCCCC
EcoT22I 311 ATGCAT ATGCA^T
1773 ATGCAT
FspI 707 TGCGCA TGC^GCA
827 TGCGCA
HapII 1053 CCGG C^CGG
1500 CCGG
Hin1I 955 GGCGCC G[GA]^CG[CT]C
1720 GACGCC
HincII 9 GTTGAC GT[CT]^[GA]AC
1480 GTCAAC
HindII 9 GTTGAC GT[CT]^[GA]AC
1480 GTCAAC
HpaII 1053 CCGG C^CGG
1500 CCGG
Ksp632I 487 CTCTTC CTCTTC(1/4)
820 CTCTTC
MluNI 893 TGGCCA TGG^CCA
1130 TGGCCA
MscI 893 TGGCCA TGG^CCA
1130 TGGCCA
MseI 98 TTAA T^TAA
140 TTAA
MspI 1053 CCGG C^CGG
1500 CCGG
NciI 1053 CCGGG CC^[GC]GG
1499 CCCGG
NcoI 599 CCATGG C^CATGG
1196 CCATGG
NsiI 311 ATGCAT ATGCA^T
1773 ATGCAT
SfcI 1667 CTGCAG C^T[GA][CT]AG
2385 CTACAG
Tru9I 98 TTAA T^TAA
140 TTAA
XcmI 1413 CCAAAATGTAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1745 CCATTGCCTGTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AvaII BbvI BmyI BpmI
BsaAI BsaJI BsgI BsiYI BslI
BsmFI Bsp1286I Bsp1407I BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr13I
DdeI DpnII EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HgaI
HhaI HinP1I HinfI HphI ItaI
MaeII MaeIII MboI MboII MflI
MnlI MslI MvaI MvnI MwoI
NdeII NlaIII NlaIV NspI PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SspBI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII