Restriction map for
pFN21AE1199
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII AgeI AhdI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BalI BamHI BbrPI BbsI BbvCI
BclI BfaI BfrI BlnI BpuAI
BsaAI BsaBI BsaWI BseAI BsgI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BstBI BstEII BstPI
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HindIII
HpaI KpnI MaeI MamI MfeI
MluI MluNI MroI MscI MseI
MunI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PflFI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tsp509I Tth111I XbaI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AccI 427 GTATAC GT^[AC][GT]AC
AccII 919 CGCG CG^CG
AcyI 922 GGCGCC G[GA]^CG[CT]C
Alw44I 547 GTGCAC G^TGCAC
AlwI 364 GATCC GGATC(4/5)
AlwNI 398 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 316 CCTGAGG CC^T[ACGT]AGG
ApaLI 547 GTGCAC G^TGCAC
BanI 922 GGCGCC G^G[CT][GA]CC
BbeI 922 GGCGCC GGCGC^C
BcgI 275 GCATTGGCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 390 GTATCC GTATCC(6/5)
BglI 1047 GCCTCTGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 20 AGATCT A^GATCT
BlpI 102 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 102 GCTGAGC GC^T[ACGT]AGC
BsaHI 922 GGCGCC G[GA]^CG[CT]C
BsaI 782 GAGACC GGTCTC(1/5)
BsmBI 256 GAGACG CGTCTC(1/5)
BsrFI 1073 GCCGGC [GA]^CCGG[CT]
BssSI 226 CACGAG CACGAG(-5/-1)
Bst1107I 427 GTATAC GTA^TAC
BstAPI 397 GCAGCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 919 CGCG CG^CG
BstXI 873 CCAGCCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 20 AGATCT [GA]^GATC[CT]
BstZ17I 427 GTATAC GTA^TAC
Bsu36I 316 CCTGAGG CC^T[ACGT]AGG
CelII 102 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1073 GCCGGC [GA]^CCGG[CT]
DsaI 918 CCGCGG C^C[GA][CT]GG
EaeI 1085 TGGCCG [CT]^GGCC[GA]
Eco81I 316 CCTGAGG CC^T[ACGT]AGG
EcoNI 894 CCTGGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1082 CCTTGG C^C[AT][AT]GG
HgaI 258 GACGC GACGC(5/10)
Hin1I 922 GGCGCC G[GA]^CG[CT]C
HinfI 45 GAATC G^A[ACGT]TC
KasI 922 GGCGCC G^GCGCC
KspI 918 CCGCGG CCGC^GG
MaeII 58 ACGT A^CGT
MflI 20 AGATCT [GA]^GATC[CT]
MslI 177 CACGGACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 918 CCGCGG C[AC]G^C[GT]G
MvnI 919 CGCG CG^CG
NaeI 1073 GCCGGC GCC^GGC
NarI 922 GGCGCC GG^CGCC
NgoMIV 1073 GCCGGC G^CCGGC
NlaIII 183 CATG CATG^
PflMI 766 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 906 GGGTCCC [GA]G^G[AT]CC[CT]
SacII 918 CCGCGG CCGC^GG
SfoI 922 GGCGCC GGC^GCC
SmaI 979 CCCGGG CCC^GGG
StyI 1082 CCTTGG C^C[AT][AT]GG
TfiI 45 GAATC G^A[AT]TC
Van91I 766 CCAGAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 20 AGATCT [GA]^GATC[CT]
XmaCI 979 CCCGGG C^CCGGG
XmaI 979 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AspHI 109 GTGCTC G[AT]GC[AT]^C
547 GTGCAC
BpmI 672 CTCCAG CTGGAG(16/14)
298 CTGGAG
BsiHKAI 109 GTGCTC G[AT]GC[AT]^C
547 GTGCAC
BsmAI 256 GAGAC GTCTC(1/5)
782 GAGAC
DpnII 21 GATC ^GATC
364 GATC
EarI 160 CTCTTC CTCTTC(1/4)
556 CTCTTC
Ksp632I 160 CTCTTC CTCTTC(1/4)
556 CTCTTC
MboI 21 GATC ^GATC
364 GATC
NdeII 21 GATC ^GATC
364 GATC
PaeR7I 253 CTCGAG C^TCGAG
840 CTCGAG
PstI 395 CTGCAG CTGCA^G
694 CTGCAG
Sau3AI 21 GATC ^GATC
364 GATC
SfaNI 29 GCATC GCATC(5/9)
950 GCATC
SmlI 253 CTCGAG C^T[CT][GA]AG
840 CTCGAG
StuI 92 AGGCCT AGG^CCT
540 AGGCCT
XcmI 173 CCAGCACGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
413 CCAGCCCAGATGTGG
XhoI 253 CTCGAG C^TCGAG
840 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AluI ApaI AvaI
AvaII BanII BbvI BcnI BmyI
BsaJI BseRI BsiYI BslI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DraII EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI HinP1I HpaII HphI ItaI
MaeIII MboII MnlI MspI MvaI
MwoI NciI NlaIV RsaI Sau96I
ScrFI SfcI TaqI TseI Tsp45I
TspRI TthHB8I