Restriction map for
pFN21AE1882
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# NO CUT ENZYMES ( 147 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AflII AflIII AgeI AhdI
ApaI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BamHI BbrPI BbsI
BbvCI BcgI BfrI BglI BglII
BlnI BpuAI BsaAI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstYI BstZ17I Cfr10I ClaI
CpoI DraI DraIII DrdI DsaI
EagI Eam1105I EarI EclXI Eco52I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FseI FspI HgaI HincII
HindII HindIII HpaI KpnI Ksp632I
KspI MfeI MflI MluI MroI
MseI MunI MvnI NaeI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI Tru9I Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 752 GTCTAC GT^[AC][GT]AC
AcyI 511 GGCGCC G[GA]^CG[CT]C
Alw44I 925 GTGCAC G^TGCAC
AocI 76 CCTGAGG CC^T[ACGT]AGG
Aor51HI 950 AGCGCT AGC^GCT
ApaLI 925 GTGCAC G^TGCAC
AspHI 925 GTGCAC G[AT]GC[AT]^C
BalI 1145 TGGCCA TGG^CCA
BanI 511 GGCGCC G^G[CT][GA]CC
BanII 957 GAGCCC G[GA]GC[CT]^C
BbeI 511 GGCGCC GGCGC^C
BciVI 1059 GGATAC GTATCC(6/5)
BcnI 28 CCCGG CC^[GC]GG
BlpI 520 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 520 GCTAAGC GC^T[ACGT]AGC
BsaBI 239 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 511 GGCGCC G[GA]^CG[CT]C
BsiHKAI 925 GTGCAC G[AT]GC[AT]^C
BspMI 748 GCAGGT ACCTGC(4/8)
BsrI 586 ACTGG ACTGG(1/-1)
BssSI 1018 CACGAG CACGAG(-5/-1)
BstAPI 1023 GCAAGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 76 CCTGAGG CC^T[ACGT]AGG
CelII 520 GCTAAGC GC^T[ACGT]AGC
DraII 589 GGGACCC [GA]G^G[ACGT]CC[CT]
EaeI 1145 TGGCCA [CT]^GGCC[GA]
Eco47III 950 AGCGCT AGC^GCT
Eco81I 76 CCTGAGG CC^T[ACGT]AGG
EcoO109I 589 GGGACCC [GA]G^G[ACGT]CC[CT]
HapII 29 CCGG C^CGG
Hin1I 511 GGCGCC G[GA]^CG[CT]C
HpaII 29 CCGG C^CGG
KasI 511 GGCGCC G^GCGCC
MaeII 714 ACGT A^CGT
MamI 239 GATATGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1145 TGGCCA TGG^CCA
MscI 1145 TGGCCA TGG^CCA
MslI 120 CACAAGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 29 CCGG C^CGG
NarI 511 GGCGCC GG^CGCC
NciI 28 CCCGG CC^[GC]GG
PleI 376 GAGTC GAGTC(4/5)
PpuMI 589 GGGACCC [GA]G^G[AT]CC[CT]
SfoI 511 GGCGCC GGC^GCC
SmlI 656 CTCAAG C^T[CT][GA]AG
TaqI 98 TCGA T^CGA
Tsp509I 1084 AATT ^AATT
TthHB8I 98 TCGA T^CGA
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# TWO CUT ENZYMES ( 19 enzymes) #
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AfaI 306 GTAC GT^AC
712 GTAC
AlwI 568 GGATC GGATC(4/5)
828 GGATC
BclI 84 TGATCA T^GATCA
720 TGATCA
BfaI 65 CTAG C^TAG
811 CTAG
BmyI 925 GTGCAC G[AGT]GC[ACT]^C
957 GAGCCC
BseRI 106 GAGGAG GAGGAG(10/8)
388 GAGGAG
Bsp1286I 925 GTGCAC G[AGT]GC[ACT]^C
957 GAGCCC
BsrDI 33 CATTGC GCAATG(2/0)
963 CATTGC
EcoNI 72 CCTACCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
858 CCTGGGAAAGG
FbaI 84 TGATCA T^GATCA
720 TGATCA
HaeIII 635 GGCC GG^CC
1146 GGCC
MaeI 65 CTAG C^TAG
811 CTAG
MaeIII 613 GTCAC ^GT[ACGT]AC
1000 GTCAC
MboII 901 GAAGA GAAGA(8/7)
1188 GAAGA
PstI 535 CTGCAG CTGCA^G
746 CTGCAG
RsaI 306 GTAC GT^AC
712 GTAC
TfiI 244 GAATC G^A[AT]TC
735 GAATC
Tsp45I 613 GTCAC ^GT[GC]AC
1000 GTCAC
TspRI 368 CAGTG CA[GC]TG[ACGT][ACGT]^
585 CACTG
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# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AluI AlwNI AvaII BbvI
BpmI BsaJI BsgI BsiYI BslI
BsmAI BsmFI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
EcoRII Fnu4HI FokI HaeII HhaI
HinP1I HinfI HphI ItaI MboI
MnlI MspA1I MvaI MwoI NdeII
NlaIII NlaIV PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI TseI