Restriction map for
pFN21AE1799
##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI Alw44I ApaI
ApaLI AscI Asp700I Asp718I AvaI
AviII AvrII BamHI BbeI BbrPI
BcgI BclI BcnI BglI BlnI
BsaAI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspEI BspHI
BspLU11I BspMI BsrBI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I CpoI DraIII DrdI
DsaI EagI EclXI Eco52I EcoO65I
FbaI FseI FspI HgaI Hin1I
KasI KpnI KspI MfeI MluI
MroI MunI MvnI NarI NciI
NcoI NdeI NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI Psp1406I PvuI RcaI
RsrII SacI SacII SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccI 633 GTCGAC GT^[AC][GT]AC
AflII 2029 CTTAAG C^TTAAG
AhdI 1410 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1598 GATCC GGATC(4/5)
AocI 1016 CCTCAGG CC^T[ACGT]AGG
AspEI 1410 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2439 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 2320 GGTGCC G^G[CT][GA]CC
BbvCI 2300 CCTCAGC CCTCAGC(-5/-2)
BfrI 2029 CTTAAG C^TTAAG
BglII 676 AGATCT A^GATCT
BlpI 817 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 817 GCTGAGC GC^T[ACGT]AGC
BsaBI 677 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 372 GAGACC GGTCTC(1/5)
BsgI 1142 GTGCAG GTGCAG(16/14)
BsmAI 372 GAGAC GTCTC(1/5)
BspDI 388 ATCGAT AT^CGAT
BsrFI 1370 GCCGGC [GA]^CCGG[CT]
BstAPI 1625 GCACACAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1016 CCTCAGG CC^T[ACGT]AGG
CelII 817 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1370 GCCGGC [GA]^CCGG[CT]
ClaI 388 ATCGAT AT^CGAT
DraI 1077 TTTAAA TTT^AAA
Eam1105I 1410 GACCCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1336 GAAGAG CTCTTC(1/4)
Eco81I 1016 CCTCAGG CC^T[ACGT]AGG
EcoNI 223 CCTACAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 385 GATATC GAT^ATC
HapII 1371 CCGG C^CGG
HpaI 2082 GTTAAC GTT^AAC
HpaII 1371 CCGG C^CGG
Ksp632I 1336 GAAGAG CTCTTC(1/4)
MaeII 631 ACGT A^CGT
MamI 677 GATCTTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspI 1371 CCGG C^CGG
NaeI 1370 GCCGGC GCC^GGC
NgoMIV 1370 GCCGGC G^CCGGC
PflFI 2439 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 744 CCAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 1357 GACCCCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 2122 CTGCAG CTGCA^G
SalI 633 GTCGAC G^TCGAC
ScaI 607 AGTACT AGT^ACT
StuI 2506 AGGCCT AGG^CCT
Tth111I 2439 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 744 CCAACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 180 CCAGCCTTGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AlwNI 152 CAGTTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1220 CAGAATCTG
Aor51HI 662 AGCGCT AGC^GCT
2108 AGCGCT
AseI 787 ATTAAT AT^TAAT
882 ATTAAT
AsnI 787 ATTAAT AT^TAAT
882 ATTAAT
AspHI 1651 GAGCAC G[AT]GC[AT]^C
2183 GTGCTC
BalI 444 TGGCCA TGG^CCA
1829 TGGCCA
BanII 1328 GGGCTC G[GA]GC[CT]^C
1527 GAGCCC
BbsI 331 GAAGAC GAAGAC(2/6)
346 GAAGAC
BciVI 1804 GGATAC GTATCC(6/5)
2207 GGATAC
BfaI 653 CTAG C^TAG
1171 CTAG
BpuAI 331 GAAGAC GAAGAC(2/6)
346 GAAGAC
BsiHKAI 1651 GAGCAC G[AT]GC[AT]^C
2183 GTGCTC
Bsp1407I 1787 TGTACA T^GTACA
2064 TGTACA
BsrGI 1787 TGTACA T^GTACA
2064 TGTACA
BssKI 564 CCAGG ^CC[ACGT]GG
1214 CCTGG
BstNI 564 CCAGG CC^[AT]GG
1214 CCTGG
BstYI 676 AGATCT [GA]^GATC[CT]
1597 AGATCC
DpnII 677 GATC ^GATC
1598 GATC
DraII 1408 GGGACCC [GA]G^G[ACGT]CC[CT]
1998 AGGTCCT
EaeI 444 TGGCCA [CT]^GGCC[GA]
1829 TGGCCA
Eco47III 662 AGCGCT AGC^GCT
2108 AGCGCT
EcoO109I 1408 GGGACCC [GA]G^G[ACGT]CC[CT]
1998 AGGTCCT
EcoRI 1705 GAATTC G^AATTC
2127 GAATTC
EcoRII 564 CCAGG ^CC[AT]GG
1214 CCTGG
EcoT22I 1870 ATGCAT ATGCA^T
2233 ATGCAT
FauI 2057 CCCGC CCCGC(4/6)
2458 GCGGG
HindIII 12 AAGCTT A^AGCTT
2312 AAGCTT
MaeI 653 CTAG C^TAG
1171 CTAG
MboI 677 GATC ^GATC
1598 GATC
MflI 676 AGATCT [GA]^GATC[CT]
1597 AGATCC
MluNI 444 TGGCCA TGG^CCA
1829 TGGCCA
MscI 444 TGGCCA TGG^CCA
1829 TGGCCA
MvaI 564 CCAGG CC^[AT]GG
1214 CCTGG
NdeII 677 GATC ^GATC
1598 GATC
NsiI 1870 ATGCAT ATGCA^T
2233 ATGCAT
NspI 1985 GCATGT [GA]CATG^[CT]
2235 GCATGT
PpuMI 1408 GGGACCC [GA]G^G[AT]CC[CT]
1998 AGGTCCT
PshBI 787 ATTAAT AT^TAAT
882 ATTAAT
PvuII 2283 CAGCTG CAG^CTG
2303 CAGCTG
Sau3AI 677 GATC ^GATC
1598 GATC
ScrFI 564 CCAGG CC^[ACGT]GG
1214 CCTGG
SspBI 1787 TGTACA T^GTACA
2064 TGTACA
XhoII 676 AGATCT [GA]^GATC[CT]
1597 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbvI BmyI BpmI BsaJI
BseRI BsiYI BslI BsmFI BsmI
Bsp1286I BsrDI BsrI BstF5I Cac8I
CfoI Cfr13I DdeI EcoT14I Fnu4HI
FokI HaeII HaeIII HhaI HinP1I
HincII HindII HinfI HphI ItaI
MaeIII MboII MnlI MseI MslI
MspA1I MwoI NlaIII NlaIV PleI
RsaI Sau96I SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I