Restriction map for
pFN21AE1288
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflII AgeI Alw44I AocI Aor51HI
ApaLI AscI AseI AsnI Asp718I
AspHI AspI BalI BamHI BbeI
BbrPI BbvCI BcgI BclI BcnI
BfrI BglI BsaAI BsaHI BsiEI
BsiHKAI BsiWI BsmBI Bsp1407I BspDI
BsrBI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraIII DsaI EaeI EagI EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRI
EcoRV FauI FbaI FseI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MluI
MluNI MscI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI Tth111I
Van91I XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 2564 GTCTAC GT^[AC][GT]AC
AccIII 1045 TCCGGA T^CCGGA
AciI 2251 CCGC CCGC(-3/-1)
AhdI 418 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 904 GGGCCC GGGCC^C
AspEI 418 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 99 TGCGCA TGC^GCA
AvrII 247 CCTAGG C^CTAGG
BanII 904 GGGCCC G[GA]GC[CT]^C
BbsI 2175 GTCTTC GAAGAC(2/6)
BglII 370 AGATCT A^GATCT
BlnI 247 CCTAGG C^CTAGG
BlpI 1543 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1543 GCTCAGC GC^T[ACGT]AGC
BpuAI 2175 GTCTTC GAAGAC(2/6)
BsaI 156 GAGACC GGTCTC(1/5)
BsaWI 1045 TCCGGA [AT]^CCGG[AT]
BseAI 1045 TCCGGA T^CCGGA
BsgI 817 GTGCAG GTGCAG(16/14)
BsmI 2126 GCATTC GAATGC(1/-1)
BspEI 1045 TCCGGA T^CCGGA
BspHI 2234 TCATGA T^CATGA
BssSI 20 CTCGTG CACGAG(-5/-1)
BstXI 1713 CCATAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1543 GCTCAGC GC^T[ACGT]AGC
CfoI 100 GCGC GCG^C
DrdI 1805 GACCAAAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 418 GACAAGGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1159 CCTACCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 2321 ATGCAT ATGCA^T
FspI 99 TGCGCA TGC^GCA
HapII 1046 CCGG C^CGG
HhaI 100 GCGC GCG^C
HinP1I 100 GCGC G^CGC
HpaII 1046 CCGG C^CGG
MfeI 1681 CAATTG C^AATTG
MroI 1045 TCCGGA T^CCGGA
MslI 2229 CACTTTCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 1046 CCGG C^CGG
MunI 1681 CAATTG C^AATTG
MwoI 1983 GCTGGAACAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NsiI 2321 ATGCAT ATGCA^T
PaeR7I 1537 CTCGAG C^TCGAG
PshAI 1018 GACACAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 569 CTGCAG CTGCA^G
RcaI 2234 TCATGA T^CATGA
SspI 1384 AATATT AAT^ATT
Tsp45I 1325 GTGAC ^GT[GC]AC
XbaI 1071 TCTAGA T^CTAGA
XhoI 1537 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 32 enzymes) #
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AflIII 870 ACATGT A^C[GA][CT]GT
2448 ACATGT
AlwI 1450 GATCC GGATC(4/5)
1822 GATCC
Asp700I 2097 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2335 GAAAGTATTC
AvaI 227 CCCGAG C^[CT]CG[GA]G
1537 CTCGAG
BbvI 1153 GCAGC GCAGC(8/12)
4 GCTGC
BciVI 729 GGATAC GTATCC(6/5)
735 GGATAC
BmyI 904 GGGCCC G[AGT]GC[ACT]^C
2658 GGGCAC
BpmI 1633 CTCCAG CTGGAG(16/14)
2522 CTCCAG
BsaBI 137 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1444 GATGATGATC
BsoBI 227 CCCGAG C^[CT]CG[GA]G
1537 CTCGAG
Bsp1286I 904 GGGCCC G[AGT]GC[ACT]^C
2658 GGGCAC
BspLU11I 870 ACATGT A^CATGT
2448 ACATGT
BspMI 1797 ACCTGC ACCTGC(4/8)
819 GCAGGT
BsrDI 96 CATTGC GCAATG(2/0)
2506 CATTGC
BstYI 370 AGATCT [GA]^GATC[CT]
1821 AGATCC
DraII 903 AGGGCCC [GA]G^G[ACGT]CC[CT]
904 GGGCCCT
EcoO109I 903 AGGGCCC [GA]G^G[ACGT]CC[CT]
904 GGGCCCT
EcoT14I 247 CCTAGG C^C[AT][AT]GG
1719 CCTTGG
Fnu4HI 4 GCTGC GC^[ACGT]GC
1153 GCAGC
HaeIII 557 GGCC GG^CC
905 GGCC
HgaI 1916 GACGC GACGC(5/10)
2030 GACGC
ItaI 4 GCTGC GC^[ACGT]GC
1153 GCAGC
MamI 137 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1444 GATGATGATC
MflI 370 AGATCT [GA]^GATC[CT]
1821 AGATCC
MspA1I 629 CAGCTG C[AC]G^C[GT]G
880 CAGCTG
NspI 870 ACATGT [GA]CATG^[CT]
2448 ACATGT
PvuII 629 CAGCTG CAG^CTG
880 CAGCTG
StyI 247 CCTAGG C^C[AT][AT]GG
1719 CCTTGG
TseI 4 GCTGC G^C[AT]GC
1153 GCAGC
XcmI 1004 CCAATAACGAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1613 CCATTGGATTTGTGG
XhoII 370 AGATCT [GA]^GATC[CT]
1821 AGATCC
XmnI 2097 GAAGTATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2335 GAAAGTATTC
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# 3 OR MORE CUT ENZYMES ( 53 enzymes) #
#########################################
AcsI AfaI AluI AlwNI ApoI
AvaII BanI BfaI BsaJI BseRI
BsiYI BslI BsmAI BsmFI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraI EarI EcoRII
FokI HinfI HphI Ksp632I MaeI
MaeIII MboI MboII MnlI MseI
MvaI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI TaqI TfiI Tru9I
Tsp509I TspRI TthHB8I