Restriction map for
pFN21AB5739
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# NO CUT ENZYMES ( 91 enzymes) #
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AatII Acc65I AccI AccIII AflII
AgeI Alw44I Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AviII
AvrII BamHI BcgI BclI BfrI
BglII BlnI BlpI Bpu1102I BsaI
BsaWI BseAI BsiWI BspDI BspEI
BspLU11I BsrDI BssHII Bst1107I BstBI
BstEII BstPI BstYI BstZ17I CelII
ClaI DraI DraIII DrdI Eco47III
EcoO65I EcoRV FauI FbaI FspI
HindIII HpaI KpnI MfeI MflI
MluI MroI MunI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmeI PpuMI PshAI
PshBI PvuI SacI SalI SbfI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspI SwaI
Van91I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccII 95 CGCG CG^CG
AclI 1753 AACGTT AA^CGTT
AflIII 1079 ACACGT A^C[GA][CT]GT
AhdI 2290 GACCGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1456 CCTGAGG CC^T[ACGT]AGG
Asp700I 603 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2290 GACCGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2265 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 1583 CTCGGG C^[CT]CG[GA]G
BalI 1022 TGGCCA TGG^CCA
BanII 2329 GAGCCC G[GA]GC[CT]^C
BbeI 9 GGCGCC GGCGC^C
BbrPI 2005 CACGTG CAC^GTG
BbsI 603 GAAGAC GAAGAC(2/6)
BbvCI 223 CCTCAGC CCTCAGC(-5/-2)
BglI 58 GCCACAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 603 GAAGAC GAAGAC(2/6)
BsaAI 2005 CACGTG [CT]AC^GT[GA]
BsaBI 444 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmBI 45 GAGACG CGTCTC(1/5)
BsoBI 1583 CTCGGG C^[CT]CG[GA]G
BspHI 1538 TCATGA T^CATGA
BssSI 1381 CACGAG CACGAG(-5/-1)
BstAPI 2190 GCACTACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 95 CGCG CG^CG
BstXI 1847 CCAACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1456 CCTGAGG CC^T[ACGT]AGG
CpoI 2288 CGGACCG CG^G[AT]CCG
Eam1105I 2290 GACCGGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1456 CCTGAGG CC^T[ACGT]AGG
EcoNI 2196 CCTGCACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 218 GAATTC G^AATTC
FseI 88 GGCCGGCC GGCCGG^CC
HaeII 9 GGCGCC [GA]GCGC^[CT]
HincII 933 GTCAAC GT[CT]^[GA]AC
HindII 933 GTCAAC GT[CT]^[GA]AC
KasI 9 GGCGCC G^GCGCC
KspI 94 CCGCGG CCGC^GG
MamI 444 GATCTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1022 TGGCCA TGG^CCA
MscI 1022 TGGCCA TGG^CCA
MvnI 95 CGCG CG^CG
NarI 9 GGCGCC GG^CGCC
NspI 1850 ACATGC [GA]CATG^[CT]
PflFI 2265 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2005 CACGTG CAC^GTG
PmlI 2005 CACGTG CAC^GTG
Psp1406I 1753 AACGTT AA^CGTT
RcaI 1538 TCATGA T^CATGA
RsrII 2288 CGGACCG CG^G[AT]CCG
SacII 94 CCGCGG CCGC^GG
SapI 822 GAAGAGC GCTCTTC(1/4)
ScaI 1391 AGTACT AGT^ACT
SfoI 9 GGCGCC GGC^GCC
Tth111I 2265 GACAGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 603 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcyI 9 GGCGCC G[GA]^CG[CT]C
1178 GGCGTC
BanI 9 GGCGCC G^G[CT][GA]CC
1959 GGTGCC
BpmI 1711 CTCCAG CTGGAG(16/14)
29 CTGGAG
BsaHI 9 GGCGCC G[GA]^CG[CT]C
1178 GGCGTC
Bsp1407I 526 TGTACA T^GTACA
1133 TGTACA
BsrBI 895 GAGCGG CCGCTC(-3/-3)
2083 GAGCGG
BsrGI 526 TGTACA T^GTACA
1133 TGTACA
DraII 146 GGGGCCT [GA]G^G[ACGT]CC[CT]
837 GGGGCCT
EcoO109I 146 GGGGCCT [GA]G^G[ACGT]CC[CT]
837 GGGGCCT
EcoT22I 1120 ATGCAT ATGCA^T
1760 ATGCAT
HgaI 2013 GACGC GACGC(5/10)
1179 GCGTC
Hin1I 9 GGCGCC G[GA]^CG[CT]C
1178 GGCGTC
NaeI 34 GCCGGC GCC^GGC
89 GCCGGC
NcoI 317 CCATGG C^CATGG
1293 CCATGG
NgoMIV 34 GCCGGC G^CCGGC
89 GCCGGC
NsiI 1120 ATGCAT ATGCA^T
1760 ATGCAT
SexAI 1190 ACCAGGT A^CC[AT]GGT
1895 ACCAGGT
SspBI 526 TGTACA T^GTACA
1133 TGTACA
StuI 436 AGGCCT AGG^CCT
1650 AGGCCT
TaqI 710 TCGA T^CGA
918 TCGA
TfiI 741 GATTC G^A[AT]TC
1772 GATTC
TthHB8I 710 TCGA T^CGA
918 TCGA
XcmI 317 CCATGGAGGCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
755 CCATTTCAGAGATGG
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# 3 OR MORE CUT ENZYMES ( 88 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BbvI
BciVI BcnI BfaI BmyI BsaJI
BseRI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DsaI EaeI EagI
EarI EclXI Eco52I EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI Tru9I TseI
Tsp45I Tsp509I TspRI