Restriction map for
pFN21AE1880
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# NO CUT ENZYMES ( 155 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaII AvrII BalI
BbeI BbrPI BbvCI BciVI BcnI
BfaI BglI BglII BlnI BlpI
Bpu1102I BsaAI BsaHI BsaI BsaWI
BseAI BsiEI BsiWI BsmAI BsmBI
BsmI BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstPI BstUI BstXI
Bsu36I CelII Cfr10I Cfr13I ClaI
CpoI DraII DraIII DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRI EcoT14I
FseI HaeII HaeIII HgaI Hin1I
HincII HindII HpaI KasI KpnI
KspI MaeI MaeII MfeI MluI
MluNI MroI MscI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsrII SacI SacII SalI
Sau96I SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI StuI StyI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccI 193 GTATAC GT^[AC][GT]AC
AcsI 871 AAATTT [GA]^AATT[CT]
AflII 1122 CTTAAG C^TTAAG
ApoI 871 AAATTT [GA]^AATT[CT]
BamHI 482 GGATCC G^GATCC
BanI 562 GGTGCC G^G[CT][GA]CC
BclI 572 TGATCA T^GATCA
BfrI 1122 CTTAAG C^TTAAG
BpmI 1090 CTGGAG CTGGAG(16/14)
BsaBI 786 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1093 GAGGAG GAGGAG(10/8)
Bsp1407I 371 TGTACA T^GTACA
BspMI 1048 ACCTGC ACCTGC(4/8)
BsrGI 371 TGTACA T^GTACA
Bst1107I 193 GTATAC GTA^TAC
BstZ17I 193 GTATAC GTA^TAC
DraI 868 TTTAAA TTT^AAA
DsaI 596 CCACGG C^C[GA][CT]GG
EcoNI 1089 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 79 GATATC GAT^ATC
EcoT22I 924 ATGCAT ATGCA^T
FauI 1063 GCGGG CCCGC(4/6)
FbaI 572 TGATCA T^GATCA
HapII 463 CCGG C^CGG
HindIII 1125 AAGCTT A^AGCTT
HpaII 463 CCGG C^CGG
MamI 786 GATGCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MspA1I 478 CAGCGG C[AC]G^C[GT]G
MspI 463 CCGG C^CGG
NsiI 924 ATGCAT ATGCA^T
NspI 1025 GCATGT [GA]CATG^[CT]
PleI 58 GACTC GAGTC(4/5)
SapI 972 GAAGAGC GCTCTTC(1/4)
SexAI 1102 ACCTGGT A^CC[AT]GGT
SfcI 980 CTGTAG C^T[GA][CT]AG
SmlI 1122 CTTAAG C^T[CT][GA]AG
SspBI 371 TGTACA T^GTACA
TfiI 459 GAATC G^A[AT]TC
XcmI 729 CCAGGTAGATATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AciI 480 GCGG CCGC(-3/-1)
1063 GCGG
AfaI 372 GTAC GT^AC
1035 GTAC
AspHI 133 GAGCAC G[AT]GC[AT]^C
310 GAGCAC
AvaI 109 CCCGAG C^[CT]CG[GA]G
286 CCCGAG
AviII 173 TGCGCA TGC^GCA
350 TGCGCA
BanII 742 GGGCTC G[GA]GC[CT]^C
975 GAGCCC
BcgI 135 GCACTCGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
312 GCACTCGGTTCG
BsgI 475 GTGCAG GTGCAG(16/14)
988 GTGCAG
BsiHKAI 133 GAGCAC G[AT]GC[AT]^C
310 GAGCAC
BsmFI 39 GGGAC GGGAC(10/14)
952 GTCCC
BsoBI 109 CCCGAG C^[CT]CG[GA]G
286 CCCGAG
BstF5I 1182 CATCC GGATG(2/0)
501 GGATG
BstYI 482 GGATCC [GA]^GATC[CT]
1118 GGATCT
CfoI 174 GCGC GCG^C
351 GCGC
EarI 610 CTCTTC CTCTTC(1/4)
972 GAAGAG
FokI 1182 CATCC GGATG(9/13)
501 GGATG
FspI 173 TGCGCA TGC^GCA
350 TGCGCA
HhaI 174 GCGC GCG^C
351 GCGC
HinP1I 174 GCGC G^CGC
351 GCGC
HinfI 58 GACTC G^A[ACGT]TC
459 GAATC
HphI 1173 GGTGA GGTGA(8/7)
554 TCACC
Ksp632I 610 CTCTTC CTCTTC(1/4)
972 GAAGAG
MaeIII 73 GTCAC ^GT[ACGT]AC
1174 GTGAC
MflI 482 GGATCC [GA]^GATC[CT]
1118 GGATCT
NlaIV 482 GGATCC GG[ACGT]^[ACGT]CC
562 GGTGCC
RsaI 372 GTAC GT^AC
1035 GTAC
Tsp45I 73 GTCAC ^GT[GC]AC
1174 GTGAC
Tsp509I 865 AATT ^AATT
872 AATT
XhoII 482 GGATCC [GA]^GATC[CT]
1118 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AluI AlwI BbsI BbvI BmyI
BpuAI BsaJI BsiYI BslI Bsp1286I
BsrDI BsrI BssKI BstNI Cac8I
DdeI DpnII EcoRII Fnu4HI ItaI
MboI MboII MnlI MseI MslI
MvaI MwoI NdeII NlaIII Sau3AI
ScrFI SfaNI Tru9I TseI TspRI