Restriction map for
pFN21AE1876
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# NO CUT ENZYMES ( 143 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AfaI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AviII AvrII BalI BanII
BbrPI BbvCI BcgI BciVI BfrI
BglI BglII BlnI BlpI BmyI
Bpu1102I BsaAI BsaWI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsmI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoNI EcoO65I EcoRI EcoT22I FseI
FspI HgaI HincII HindII HpaI
KpnI Ksp632I KspI MaeII MluI
MluNI MroI MscI MspA1I NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TaqI Tsp45I TthHB8I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccII 1155 CGCG CG^CG
AcyI 910 GGCGCC G[GA]^CG[CT]C
AspI 936 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 850 CCCGAG C^[CT]CG[GA]G
BanI 910 GGCGCC G^G[CT][GA]CC
BbeI 910 GGCGCC GGCGC^C
BbsI 273 GAAGAC GAAGAC(2/6)
BclI 534 TGATCA T^GATCA
BpmI 1171 CTGGAG CTGGAG(16/14)
BpuAI 273 GAAGAC GAAGAC(2/6)
BsaBI 469 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 910 GGCGCC G[GA]^CG[CT]C
BsaI 941 GGTCTC GGTCTC(1/5)
BseRI 251 CTCCTC GAGGAG(10/8)
BsmFI 73 GGGAC GGGAC(10/14)
BsoBI 850 CCCGAG C^[CT]CG[GA]G
BspMI 267 GCAGGT ACCTGC(4/8)
BsrBI 1174 GAGCGG CCGCTC(-3/-3)
BsrDI 93 CATTGC GCAATG(2/0)
BssSI 187 CACGAG CACGAG(-5/-1)
BstUI 1155 CGCG CG^CG
DraII 73 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoO109I 73 GGGACCC [GA]G^G[ACGT]CC[CT]
EcoRV 368 GATATC GAT^ATC
EcoT14I 240 CCATGG C^C[AT][AT]GG
FauI 146 CCCGC CCCGC(4/6)
FbaI 534 TGATCA T^GATCA
Hin1I 910 GGCGCC G[GA]^CG[CT]C
HindIII 287 AAGCTT A^AGCTT
KasI 910 GGCGCC G^GCGCC
MaeIII 1151 GTAAC ^GT[ACGT]AC
MamI 469 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1089 CAATTG C^AATTG
MseI 950 TTAA T^TAA
MslI 427 CATTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1089 CAATTG C^AATTG
MvnI 1155 CGCG CG^CG
NarI 910 GGCGCC GG^CGCC
NcoI 240 CCATGG C^CATGG
PflFI 936 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 73 GGGACCC [GA]G^G[AT]CC[CT]
PstI 505 CTGCAG CTGCA^G
SexAI 113 ACCAGGT A^CC[AT]GGT
SfoI 910 GGCGCC GGC^GCC
SmlI 876 CTTGAG C^T[CT][GA]AG
StyI 240 CCATGG C^C[AT][AT]GG
TfiI 791 GAATC G^A[AT]TC
Tru9I 950 TTAA T^TAA
Tth111I 936 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AciI 147 CCGC CCGC(-3/-1)
1176 GCGG
AcsI 567 GAATTT [GA]^AATT[CT]
1054 GAATTT
Aor51HI 527 AGCGCT AGC^GCT
587 AGCGCT
ApoI 567 GAATTT [GA]^AATT[CT]
1054 GAATTT
Asp700I 273 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
564 GAAGAATTTC
AvaII 48 GGACC G^G[AT]CC
74 GGACC
BamHI 35 GGATCC G^GATCC
1011 GGATCC
BcnI 165 CCCGG CC^[GC]GG
956 CCCGG
BsmAI 226 GAGAC GTCTC(1/5)
942 GTCTC
BstF5I 1026 CATCC GGATG(2/0)
786 GGATG
Cac8I 82 GCTCGC GC[ACGT]^[ACGT]GC
289 GCTTGC
Cfr13I 48 GGACC G^G[ACGT]CC
74 GGACC
DsaI 240 CCATGG C^C[GA][CT]GG
782 CCGTGG
EaeI 167 CGGCCA [CT]^GGCC[GA]
779 TGGCCG
Eco47III 527 AGCGCT AGC^GCT
587 AGCGCT
FokI 1026 CATCC GGATG(9/13)
786 GGATG
HapII 166 CCGG C^CGG
957 CCGG
HpaII 166 CCGG C^CGG
957 CCGG
MspI 166 CCGG C^CGG
957 CCGG
NciI 165 CCCGG CC^[GC]GG
956 CCCGG
PflMI 39 CCATAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
216 CCAGGATTTGG
PleI 129 GAGTC GAGTC(4/5)
657 GAGTC
Sau96I 48 GGACC G^G[ACGT]CC
74 GGACC
SfcI 505 CTGCAG C^T[GA][CT]AG
1115 CTACAG
Van91I 39 CCATAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
216 CCAGGATTTGG
XmnI 273 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
564 GAAGAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AluI AlwI BbvI BfaI BsaJI
BsiYI BslI BsrI BssKI BstNI
BstYI CfoI DdeI DpnII EcoRII
Fnu4HI HaeII HaeIII HhaI HinP1I
HinfI HphI ItaI MaeI MboI
MboII MflI MnlI MvaI MwoI
NdeII NlaIII NlaIV Sau3AI ScrFI
SfaNI TseI Tsp509I TspRI XhoII