Restriction map for
pFN21AE2051
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# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Aor51HI ApaI AscI AseI
AsnI AspEI AspI AviII AvrII
BamHI BbeI BciVI BclI BfaI
BfrI BglII BlnI BsaBI BsaI
BseAI BsiWI BsmAI BsmBI BsmI
Bsp1407I BspEI BsrGI BssHII Bst1107I
BstBI BstZ17I CpoI DraI DraIII
Eam1105I Eco47III EcoNI EcoRI EcoT22I
FbaI FseI FspI HindIII HpaI
KasI MaeI MamI MfeI MluI
MroI MseI MunI NarI NdeI
NheI NotI NruI NsiI NspV
PacI PflFI PinAI PleI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacI SalI SbfI SfoI SfuI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tru9I Tth111I XbaI
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# ONE CUT ENZYMES ( 51 enzymes) #
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Acc65I 242 GGTACC G^GTACC
AcsI 371 AAATTC [GA]^AATT[CT]
AcyI 267 GACGCC G[GA]^CG[CT]C
Alw44I 1168 GTGCAC G^TGCAC
ApaLI 1168 GTGCAC G^TGCAC
ApoI 371 AAATTC [GA]^AATT[CT]
Asp718I 242 GGTACC G^GTACC
AspHI 1168 GTGCAC G[AT]GC[AT]^C
BanI 242 GGTACC G^G[CT][GA]CC
BbvCI 69 CCTCAGC CCTCAGC(-5/-2)
BcgI 1745 GCAAGAACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaHI 267 GACGCC G[GA]^CG[CT]C
BsaWI 1826 ACCGGA [AT]^CCGG[AT]
BseRI 1501 GAGGAG GAGGAG(10/8)
BsiEI 1457 CGGCCG CG[GA][CT]^CG
BsiHKAI 1168 GTGCAC G[AT]GC[AT]^C
BspHI 668 TCATGA T^CATGA
BspLU11I 587 ACATGT A^CATGT
BspMI 107 ACCTGC ACCTGC(4/8)
BssSI 76 CTCGTG CACGAG(-5/-1)
BstAPI 1302 GCAGATGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 191 AGATCC [GA]^GATC[CT]
DrdI 1075 GACTTCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 1457 CGGCCG C^GGCCG
EarI 1696 GAAGAG CTCTTC(1/4)
EclXI 1457 CGGCCG C^GGCCG
Eco52I 1457 CGGCCG C^GGCCG
EcoRV 499 GATATC GAT^ATC
HaeII 740 GGCGCT [GA]GCGC^[CT]
Hin1I 267 GACGCC G[GA]^CG[CT]C
HincII 1660 GTCAAC GT[CT]^[GA]AC
HindII 1660 GTCAAC GT[CT]^[GA]AC
KpnI 242 GGTACC GGTAC^C
Ksp632I 1696 GAAGAG CTCTTC(1/4)
KspI 1655 CCGCGG CCGC^GG
MflI 191 AGATCC [GA]^GATC[CT]
NaeI 46 GCCGGC GCC^GGC
NcoI 1730 CCATGG C^CATGG
NgoMIV 46 GCCGGC G^CCGGC
PaeR7I 1753 CTCGAG C^TCGAG
RcaI 668 TCATGA T^CATGA
SacII 1655 CCGCGG CCGC^GG
SapI 1696 GAAGAGC GCTCTTC(1/4)
ScaI 221 AGTACT AGT^ACT
SexAI 1490 ACCAGGT A^CC[AT]GGT
SfiI 1359 GGCCATCAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SgrAI 312 CACCGGCG C[GA]^CCGG[CT]G
SphI 1550 GCATGC GCATG^C
XcmI 705 CCAGTTCCTGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1753 CTCGAG C^TCGAG
XhoII 191 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 1468 GTAGAC GT^[AC][GT]AC
1537 GTCTAC
AlwNI 706 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
910 CAGTTCCTG
AocI 333 CCTGAGG CC^T[ACGT]AGG
1568 CCTCAGG
BanII 81 GGGCTC G[GA]GC[CT]^C
1423 GGGCTC
BbrPI 1198 CACGTG CAC^GTG
1297 CACGTG
BglI 74 GCCTCGTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1360 GCCATCAAGGC
BlpI 810 GCTCAGC GC^T[ACGT]AGC
1777 GCTGAGC
BpmI 907 CTCCAG CTGGAG(16/14)
799 CTGGAG
Bpu1102I 810 GCTCAGC GC^T[ACGT]AGC
1777 GCTGAGC
BsrFI 46 GCCGGC [GA]^CCGG[CT]
313 ACCGGC
BstEII 15 GGTCACC G^GT[ACGT]ACC
771 GGTGACC
BstPI 15 GGTCACC G^GT[ACGT]ACC
771 GGTGACC
BstXI 548 CCAAAGCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
776 CCACGGAGATGG
Bsu36I 333 CCTGAGG CC^T[ACGT]AGG
1568 CCTCAGG
CelII 810 GCTCAGC GC^T[ACGT]AGC
1777 GCTGAGC
Cfr10I 46 GCCGGC [GA]^CCGG[CT]
313 ACCGGC
DraII 1325 AGGACCT [GA]G^G[ACGT]CC[CT]
1505 AGGACCC
Eco81I 333 CCTGAGG CC^T[ACGT]AGG
1568 CCTCAGG
EcoO109I 1325 AGGACCT [GA]G^G[ACGT]CC[CT]
1505 AGGACCC
EcoO65I 15 GGTCACC G^GT[ACGT]ACC
771 GGTGACC
EcoT14I 1226 CCAAGG C^C[AT][AT]GG
1730 CCATGG
HgaI 267 GACGC GACGC(5/10)
344 GACGC
PmaCI 1198 CACGTG CAC^GTG
1297 CACGTG
PmlI 1198 CACGTG CAC^GTG
1297 CACGTG
PpuMI 1325 AGGACCT [GA]G^G[AT]CC[CT]
1505 AGGACCC
PstI 93 CTGCAG CTGCA^G
1516 CTGCAG
SfcI 93 CTGCAG C^T[GA][CT]AG
1516 CTGCAG
SmaI 993 CCCGGG CCC^GGG
1370 CCCGGG
StyI 1226 CCAAGG C^C[AT][AT]GG
1730 CCATGG
Tsp509I 372 AATT ^AATT
541 AATT
XmaCI 993 CCCGGG C^CCGGG
1370 CCCGGG
XmaI 993 CCCGGG C^CCGGG
1370 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI Asp700I AvaI AvaII BalI
BbsI BbvI BcnI BmyI BpuAI
BsaAI BsaJI BsgI BsiYI BslI
BsmFI BsoBI Bsp1286I BspDI BsrBI
BsrDI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I ClaI
DdeI DpnII DsaI EaeI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI TaqI TfiI TseI
Tsp45I TspRI TthHB8I Van91I XmnI