Restriction map for
pFN21AE1990
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# NO CUT ENZYMES ( 107 enzymes) #
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AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I AocI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AviII AvrII BbeI BbrPI
BbsI BbvCI BciVI BclI BfrI
BlnI BpuAI BsaAI BsaBI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BsrGI Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraI DraIII Eam1105I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KpnI MamI
MfeI MluI MroI MseI MslI
MunI NarI NcoI NdeI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI SfoI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tru9I Tth111I
XbaI XhoI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 1030 GTCTAC GT^[AC][GT]AC
AcsI 1588 GAATTT [GA]^AATT[CT]
AflIII 458 ACATGT A^C[GA][CT]GT
Aor51HI 302 AGCGCT AGC^GCT
ApoI 1588 GAATTT [GA]^AATT[CT]
Asp700I 258 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 643 CCCGGG C^[CT]CG[GA]G
BamHI 1669 GGATCC G^GATCC
BanI 1455 GGTGCC G^G[CT][GA]CC
BcgI 504 CGACAGCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaI 1627 GAGACC GGTCTC(1/5)
BsaWI 854 ACCGGA [AT]^CCGG[AT]
BsoBI 643 CCCGGG C^[CT]CG[GA]G
BspLU11I 458 ACATGT A^CATGT
BspMI 920 ACCTGC ACCTGC(4/8)
BsrBI 205 GAGCGG CCGCTC(-3/-3)
BsrDI 1043 GCAATG GCAATG(2/0)
BssHII 514 GCGCGC G^CGCGC
BssSI 618 CACGAG CACGAG(-5/-1)
DrdI 1021 GACATCAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 108 CCGCGG C^C[GA][CT]GG
EagI 451 CGGCCG C^GGCCG
EclXI 451 CGGCCG C^GGCCG
Eco47III 302 AGCGCT AGC^GCT
Eco52I 451 CGGCCG C^GGCCG
KspI 108 CCGCGG CCGC^GG
MaeII 278 ACGT A^CGT
NaeI 893 GCCGGC GCC^GGC
NgoMIV 893 GCCGGC G^CCGGC
PflMI 1311 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 1296 GAGCTC GAGCT^C
SacII 108 CCGCGG CCGC^GG
SapI 858 GAAGAGC GCTCTTC(1/4)
ScaI 905 AGTACT AGT^ACT
SfaNI 125 GCATC GCATC(5/9)
SfiI 183 GGCCAGCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 643 CCCGGG CCC^GGG
SmlI 1227 CTTGAG C^T[CT][GA]AG
TfiI 1163 GAATC G^A[AT]TC
Tsp509I 1589 AATT ^AATT
Van91I 1311 CCAGAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 32 CCAACGGGCGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 643 CCCGGG C^CCGGG
XmaI 643 CCCGGG C^CCGGG
XmnI 258 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AspHI 870 GAGCAC G[AT]GC[AT]^C
1296 GAGCTC
BalI 182 TGGCCA TGG^CCA
950 TGGCCA
BglI 184 GCCAGCTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
937 GCCTCCTTGGC
BglII 482 AGATCT A^GATCT
1346 AGATCT
BlpI 69 GCTGAGC GC^T[ACGT]AGC
390 GCTGAGC
Bpu1102I 69 GCTGAGC GC^T[ACGT]AGC
390 GCTGAGC
BsiEI 408 CGACCG CG[GA][CT]^CG
451 CGGCCG
BsiHKAI 870 GAGCAC G[AT]GC[AT]^C
1296 GAGCTC
BsrFI 893 GCCGGC [GA]^CCGG[CT]
1253 ACCGGC
BstXI 14 CCAAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1439 CCAGTTCTCTGG
CelII 69 GCTGAGC GC^T[ACGT]AGC
390 GCTGAGC
Cfr10I 893 GCCGGC [GA]^CCGG[CT]
1253 ACCGGC
EarI 1331 CTCTTC CTCTTC(1/4)
858 GAAGAG
EcoNI 252 CCTGAAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1065 CCTGGATCAGG
Ksp632I 1331 CTCTTC CTCTTC(1/4)
858 GAAGAG
MluNI 182 TGGCCA TGG^CCA
950 TGGCCA
MscI 182 TGGCCA TGG^CCA
950 TGGCCA
NheI 339 GCTAGC G^CTAGC
552 GCTAGC
NspI 458 ACATGT [GA]CATG^[CT]
977 ACATGC
PleI 1690 GACTC GAGTC(4/5)
775 GAGTC
SexAI 1034 ACCTGGT A^CC[AT]GGT
1574 ACCTGGT
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI ApaI AvaII BanII
BbvI BcnI BfaI BmyI BpmI
BsaHI BsaJI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfcI StuI StyI TaqI
TseI Tsp45I TspRI TthHB8I XhoII