Restriction map for
pFN21AE1906
##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AccIII AclI AcsI AflII AflIII
AgeI AhdI Alw44I AocI ApaI
ApaLI ApoI AscI AseI AsnI
AspEI AspI AvrII BalI BamHI
BbeI BbrPI BbvCI BfrI BglI
BglII BlnI BsaAI BsaI BsaWI
BseAI BsiWI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Bsu36I ClaI CpoI DraIII
DrdI Eam1105I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT22I FseI HgaI
HincII HindII HpaI KasI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PshBI Psp1406I PvuI
PvuII RsrII SacI SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 481 GACGTC GACGT^C
Acc65I 422 GGTACC G^GTACC
AccI 63 GTCTAC GT^[AC][GT]AC
AcyI 481 GACGTC G[GA]^CG[CT]C
AlwNI 127 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 709 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 422 GGTACC G^GTACC
AvaI 178 CCCGAG C^[CT]CG[GA]G
AviII 98 TGCGCA TGC^GCA
BanII 175 GAGCCC G[GA]GC[CT]^C
BciVI 1085 GGATAC GTATCC(6/5)
BclI 870 TGATCA T^GATCA
BcnI 192 CCCGG CC^[GC]GG
BsaBI 201 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 481 GACGTC G[GA]^CG[CT]C
BseRI 276 GAGGAG GAGGAG(10/8)
BsmAI 484 GTCTC GTCTC(1/5)
BsmBI 483 CGTCTC CGTCTC(1/5)
BsmI 501 GAATGC GAATGC(1/-1)
BsoBI 178 CCCGAG C^[CT]CG[GA]G
BspHI 705 TCATGA T^CATGA
BsrDI 490 GCAATG GCAATG(2/0)
BsrFI 974 ACCGGC [GA]^CCGG[CT]
Cfr10I 974 ACCGGC [GA]^CCGG[CT]
DraI 742 TTTAAA TTT^AAA
DraII 1169 GGGTCCT [GA]G^G[ACGT]CC[CT]
DsaI 120 CCGCGG C^C[GA][CT]GG
EaeI 257 CGGCCG [CT]^GGCC[GA]
EagI 257 CGGCCG C^GGCCG
EarI 286 GAAGAG CTCTTC(1/4)
EclXI 257 CGGCCG C^GGCCG
Eco52I 257 CGGCCG C^GGCCG
EcoO109I 1169 GGGTCCT [GA]G^G[ACGT]CC[CT]
EcoT14I 299 CCAAGG C^C[AT][AT]GG
FbaI 870 TGATCA T^GATCA
FspI 98 TGCGCA TGC^GCA
Hin1I 481 GACGTC G[GA]^CG[CT]C
HindIII 750 AAGCTT A^AGCTT
KpnI 422 GGTACC GGTAC^C
Ksp632I 286 GAAGAG CTCTTC(1/4)
KspI 120 CCGCGG CCGC^GG
MamI 201 GATTTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 4 CACTACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 120 CCGCGG C[AC]G^C[GT]G
NciI 192 CCCGG CC^[GC]GG
NotI 256 GCGGCCGC GC^GGCCGC
PpuMI 1169 GGGTCCT [GA]G^G[AT]CC[CT]
PshAI 953 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 69 CTGCAG CTGCA^G
RcaI 705 TCATGA T^CATGA
SacII 120 CCGCGG CCGC^GG
ScaI 290 AGTACT AGT^ACT
SphI 1129 GCATGC GCATG^C
StyI 299 CCAAGG C^C[AT][AT]GG
TfiI 594 GATTC G^A[AT]TC
XcmI 408 CCAGGTGCTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 709 GAATGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
Aor51HI 19 AGCGCT AGC^GCT
629 AGCGCT
AspHI 393 GTGCTC G[AT]GC[AT]^C
412 GTGCTC
AvaII 87 GGACC G^G[AT]CC
1170 GGTCC
BanI 422 GGTACC G^G[CT][GA]CC
847 GGCACC
BbsI 163 GAAGAC GAAGAC(2/6)
912 GAAGAC
BcgI 71 GCAGCACACTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
101 GCAAGAGGATCG
BlpI 15 GCTGAGC GC^T[ACGT]AGC
54 GCTCAGC
Bpu1102I 15 GCTGAGC GC^T[ACGT]AGC
54 GCTCAGC
BpuAI 163 GAAGAC GAAGAC(2/6)
912 GAAGAC
BsgI 768 CTGCAC GTGCAG(16/14)
1021 GTGCAG
BsiEI 47 CGGTCG CG[GA][CT]^CG
257 CGGCCG
BsiHKAI 393 GTGCTC G[AT]GC[AT]^C
412 GTGCTC
BspMI 67 ACCTGC ACCTGC(4/8)
835 GCAGGT
BstAPI 386 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
826 GCAAGATTTGC
BstYI 128 AGATCC [GA]^GATC[CT]
1068 GGATCT
CelII 15 GCTGAGC GC^T[ACGT]AGC
54 GCTCAGC
Cfr13I 87 GGACC G^G[ACGT]CC
1170 GGTCC
Eco47III 19 AGCGCT AGC^GCT
629 AGCGCT
FauI 438 CCCGC CCCGC(4/6)
122 GCGGG
HaeII 19 AGCGCT [GA]GCGC^[CT]
629 AGCGCT
HaeIII 258 GGCC GG^CC
1002 GGCC
MaeII 482 ACGT A^CGT
1041 ACGT
MflI 128 AGATCC [GA]^GATC[CT]
1068 GGATCT
MseI 613 TTAA T^TAA
743 TTAA
NspI 320 ACATGC [GA]CATG^[CT]
1129 GCATGC
PleI 533 GAGTC GAGTC(4/5)
958 GAGTC
Sau96I 87 GGACC G^G[ACGT]CC
1170 GGTCC
SmlI 598 CTCAAG C^T[CT][GA]AG
955 CTTGAG
Tru9I 613 TTAA T^TAA
743 TTAA
Tsp45I 43 GTCAC ^GT[GC]AC
960 GTCAC
XhoII 128 AGATCC [GA]^GATC[CT]
1068 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
BbvI BfaI BmyI BpmI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI DdeI DpnII EcoRII Fnu4HI
FokI HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MnlI MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI ScrFI SfaNI SfcI
TaqI TseI Tsp509I TspRI TthHB8I