Restriction map for
pFN21AE1932
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII Acc65I AccI AccII AciI
AclI AcyI AfaI AflII AflIII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AspEI
AspI AvaI AviII AvrII BamHI
BanI BbeI BbrPI BbvCI BciVI
BclI BfrI BlnI BlpI Bpu1102I
BsaHI BseRI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspHI
BspLU11I BspMI BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CelII
CfoI Cfr10I ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRV EcoT22I
FauI FbaI FseI FspI HaeII
HgaI HhaI Hin1I HinP1I HpaI
KasI KpnI KspI MfeI MluI
MunI MvnI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacII SalI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccIII 401 TCCGGA T^CCGGA
AlwNI 775 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 668 CCTTAGG CC^T[ACGT]AGG
ApaI 316 GGGCCC GGGCC^C
Asp700I 405 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI 1294 GTCTTC GAAGAC(2/6)
BcgI 261 GCACGCACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 227 CCGGG CC^[GC]GG
BglI 309 GCCAGGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 890 AGATCT A^GATCT
BpuAI 1294 GTCTTC GAAGAC(2/6)
BsaAI 764 TACGTG [CT]AC^GT[GA]
BsaBI 286 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 401 TCCGGA [AT]^CCGG[AT]
BseAI 401 TCCGGA T^CCGGA
BsmI 770 GCATTC GAATGC(1/-1)
BspEI 401 TCCGGA T^CCGGA
BssSI 652 CACGAG CACGAG(-5/-1)
BstXI 546 CCATGCTTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 668 CCTTAGG CC^T[ACGT]AGG
DraI 1039 TTTAAA TTT^AAA
EarI 47 CTCTTC CTCTTC(1/4)
Eco81I 668 CCTTAGG CC^T[ACGT]AGG
EcoRI 601 GAATTC G^AATTC
HindIII 407 AAGCTT A^AGCTT
Ksp632I 47 CTCTTC CTCTTC(1/4)
MamI 286 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 401 TCCGGA T^CCGGA
MspA1I 1145 CAGCTG C[AC]G^C[GT]G
NciI 227 CCGGG CC^[GC]GG
PleI 278 GAGTC GAGTC(4/5)
PpuMI 1171 AGGACCT [GA]G^G[AT]CC[CT]
PstI 25 CTGCAG CTGCA^G
PvuII 1145 CAGCTG CAG^CTG
SapI 46 GCTCTTC GCTCTTC(1/4)
SbfI 24 CCTGCAGG CCTGCA^GG
SmlI 127 CTTGAG C^T[CT][GA]AG
Sse8387I 24 CCTGCAGG CCTGCA^GG
XcmI 513 CCATGCTTTTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 405 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AspHI 44 GAGCTC G[AT]GC[AT]^C
497 GAGCTC
BfaI 541 CTAG C^TAG
686 CTAG
BpmI 41 CTGGAG CTGGAG(16/14)
250 CTGGAG
BsaI 788 GAGACC GGTCTC(1/5)
936 GAGACC
BsiHKAI 44 GAGCTC G[AT]GC[AT]^C
497 GAGCTC
BsmFI 280 GTCCC GGGAC(10/14)
777 GTCCC
BstF5I 384 GGATG GGATG(2/0)
579 GGATG
DraII 315 GGGGCCC [GA]G^G[ACGT]CC[CT]
1171 AGGACCT
DsaI 492 CCGTGG C^C[GA][CT]GG
878 CCGTGG
EcoO109I 315 GGGGCCC [GA]G^G[ACGT]CC[CT]
1171 AGGACCT
EcoT14I 1266 CCAAGG C^C[AT][AT]GG
1329 CCAAGG
FokI 384 GGATG GGATG(9/13)
579 GGATG
HapII 227 CCGG C^CGG
402 CCGG
HincII 388 GTTGAC GT[CT]^[GA]AC
727 GTTGAC
HindII 388 GTTGAC GT[CT]^[GA]AC
727 GTTGAC
HpaII 227 CCGG C^CGG
402 CCGG
MaeI 541 CTAG C^TAG
686 CTAG
MspI 227 CCGG C^CGG
402 CCGG
SacI 44 GAGCTC GAGCT^C
497 GAGCTC
SfcI 25 CTGCAG C^T[GA][CT]AG
345 CTACAG
StyI 1266 CCAAGG C^C[AT][AT]GG
1329 CCAAGG
TaqI 270 TCGA T^CGA
590 TCGA
TthHB8I 270 TCGA T^CGA
590 TCGA
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AcsI AluI AlwI ApoI AvaII
BalI BanII BbvI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstNI BstYI Cac8I Cfr13I
DdeI DpnII EaeI EcoRII Fnu4HI
HaeIII HinfI HphI ItaI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MvaI
MwoI NdeII NlaIII NlaIV Sau3AI
Sau96I ScrFI SfaNI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII