Restriction map for
pFN21AE2080
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AflII AgeI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BbeI BbvCI BclI BfrI BglII
BlnI BlpI Bpu1102I BsaWI BseAI
BsiWI BspDI BspEI BspHI BsrBI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I CelII ClaI
CpoI DraI DraIII DsaI EagI
EclXI Eco52I EcoNI EcoO65I EcoRV
EcoT14I EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KasI
KpnI KspI MluI MroI MvnI
NarI NcoI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI PvuI PvuII
RcaI RsrII SacI SacII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmlI SnaBI SpeI SphI
Sse8387I StyI SwaI Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AclI 326 AACGTT AA^CGTT
AcyI 932 GGCGTC G[GA]^CG[CT]C
AhdI 1654 GACTTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1906 CCTAAGG CC^T[ACGT]AGG
Aor51HI 1099 AGCGCT AGC^GCT
AspEI 1654 GACTTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 118 CCCGGG C^[CT]CG[GA]G
BamHI 1872 GGATCC G^GATCC
BanI 1138 GGCACC G^G[CT][GA]CC
BbrPI 778 CACGTG CAC^GTG
BbsI 2149 GAAGAC GAAGAC(2/6)
BcgI 29 GCACTGCTTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1623 GGATAC GTATCC(6/5)
BpuAI 2149 GAAGAC GAAGAC(2/6)
BsaAI 778 CACGTG [CT]AC^GT[GA]
BsaBI 274 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 932 GGCGTC G[GA]^CG[CT]C
BsiEI 2214 CGACCG CG[GA][CT]^CG
BsmI 716 GAATGC GAATGC(1/-1)
BsoBI 118 CCCGGG C^[CT]CG[GA]G
BspLU11I 1718 ACATGT A^CATGT
BspMI 1106 ACCTGC ACCTGC(4/8)
BsrDI 384 GCAATG GCAATG(2/0)
BstXI 1337 CCAAAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1906 CCTAAGG CC^T[ACGT]AGG
DrdI 1558 GACCTTCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1654 GACTTCATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1099 AGCGCT AGC^GCT
Eco81I 1906 CCTAAGG CC^T[ACGT]AGG
EcoRI 1730 GAATTC G^AATTC
HaeII 1099 AGCGCT [GA]GCGC^[CT]
Hin1I 932 GGCGTC G[GA]^CG[CT]C
MamI 274 GATGATGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 842 GCCGGC GCC^GGC
NdeI 1798 CATATG CA^TATG
NgoMIV 842 GCCGGC G^CCGGC
PflMI 1364 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 778 CACGTG CAC^GTG
PmlI 778 CACGTG CAC^GTG
Psp1406I 326 AACGTT AA^CGTT
PstI 22 CTGCAG CTGCA^G
SapI 8 GAAGAGC GCTCTTC(1/4)
SexAI 1226 ACCTGGT A^CC[AT]GGT
SmaI 118 CCCGGG CCC^GGG
SspI 1541 AATATT AAT^ATT
StuI 1011 AGGCCT AGG^CCT
Van91I 1364 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 118 CCCGGG C^CCGGG
XmaI 118 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AcsI 1463 AAATTC [GA]^AATT[CT]
1730 GAATTC
Alw44I 27 GTGCAC G^TGCAC
59 GTGCAC
AlwNI 1222 CAGCACCTG CAG[ACGT][ACGT][ACGT]^CTG
1365 CAGGACCTG
ApaI 123 GGGCCC GGGCC^C
804 GGGCCC
ApaLI 27 GTGCAC G^TGCAC
59 GTGCAC
ApoI 1463 AAATTC [GA]^AATT[CT]
1730 GAATTC
BalI 1052 TGGCCA TGG^CCA
1259 TGGCCA
BglI 837 GCCTGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
850 GCCCAGAAGGC
BsaI 1556 GAGACC GGTCTC(1/5)
233 GGTCTC
BsiYI 1364 CCAGGACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1986 CCCTTCCCAGG
BslI 1364 CCAGGACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1986 CCCTTCCCAGG
BsmBI 1126 GAGACG CGTCTC(1/5)
2210 GAGACG
Bsp1407I 1518 TGTACA T^GTACA
1817 TGTACA
BsrFI 842 GCCGGC [GA]^CCGG[CT]
2216 ACCGGC
BsrGI 1518 TGTACA T^GTACA
1817 TGTACA
BssSI 1095 CACGAG CACGAG(-5/-1)
1811 CTCGTG
BstYI 113 AGATCC [GA]^GATC[CT]
1872 GGATCC
CfoI 50 GCGC GCG^C
1100 GCGC
Cfr10I 842 GCCGGC [GA]^CCGG[CT]
2216 ACCGGC
FauI 1254 CCCGC CCCGC(4/6)
674 GCGGG
HgaI 1128 GACGC GACGC(5/10)
933 GCGTC
HhaI 50 GCGC GCG^C
1100 GCGC
HinP1I 50 GCGC G^CGC
1100 GCGC
MfeI 758 CAATTG C^AATTG
1535 CAATTG
MflI 113 AGATCC [GA]^GATC[CT]
1872 GGATCC
MluNI 1052 TGGCCA TGG^CCA
1259 TGGCCA
MscI 1052 TGGCCA TGG^CCA
1259 TGGCCA
MslI 711 CATAAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1917 CACCCTGGTG
MunI 758 CAATTG C^AATTG
1535 CAATTG
NheI 553 GCTAGC G^CTAGC
846 GCTAGC
NspI 1515 GCATGT [GA]CATG^[CT]
1718 ACATGT
PpuMI 1366 AGGACCT [GA]G^G[AT]CC[CT]
1777 GGGACCT
SfcI 22 CTGCAG C^T[GA][CT]AG
1688 CTATAG
SspBI 1518 TGTACA T^GTACA
1817 TGTACA
TaqI 237 TCGA T^CGA
1311 TCGA
TthHB8I 237 TCGA T^CGA
1311 TCGA
XcmI 1198 CCAAATCTCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1337 CCAAAGCTCTGGTGG
XhoII 113 AGATCC [GA]^GATC[CT]
1872 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AspHI AvaII BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsgI BsiHKAI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI