Restriction map for
pFN21AE1914
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# NO CUT ENZYMES ( 118 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BamHI BanI BbeI BbrPI BbsI
BbvCI BclI BfrI BlnI BpuAI
BsaAI BsaBI BsaHI BseAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspLU11I
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I Cfr10I ClaI DraI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRV EcoT22I FbaI FseI
HaeII Hin1I HincII HindII HpaI
KasI KpnI MamI MfeI MluI
MroI MunI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
SalI SapI ScaI SexAI SfiI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI SspBI SspI StuI
SwaI Tth111I XhoI
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# ONE CUT ENZYMES ( 46 enzymes) #
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Alw44I 1183 GTGCAC G^TGCAC
ApaLI 1183 GTGCAC G^TGCAC
Asp700I 492 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 749 TGCGCA TGC^GCA
BalI 27 TGGCCA TGG^CCA
BcgI 38 GCAACCAGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1255 GTATCC GTATCC(6/5)
BglII 1127 AGATCT A^GATCT
BlpI 1080 GCTGAGC GC^T[ACGT]AGC
BpmI 130 CTGGAG CTGGAG(16/14)
Bpu1102I 1080 GCTGAGC GC^T[ACGT]AGC
BsaI 1140 GGTCTC GGTCTC(1/5)
BsiEI 105 CGACCG CG[GA][CT]^CG
BsmAI 1141 GTCTC GTCTC(1/5)
BspMI 746 ACCTGC ACCTGC(4/8)
BsrBI 699 GAGCGG CCGCTC(-3/-3)
BssSI 364 CACGAG CACGAG(-5/-1)
BstXI 968 CCAAGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1080 GCTGAGC GC^T[ACGT]AGC
CfoI 750 GCGC GCG^C
CpoI 479 CGGACCG CG^G[AT]CCG
DraIII 679 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 262 GACAGTTTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1306 GAAGAG CTCTTC(1/4)
EcoNI 161 CCTACGGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 492 GAATTC G^AATTC
FspI 749 TGCGCA TGC^GCA
HgaI 503 GCGTC GACGC(5/10)
HhaI 750 GCGC GCG^C
HinP1I 750 GCGC G^CGC
HindIII 1175 AAGCTT A^AGCTT
Ksp632I 1306 GAAGAG CTCTTC(1/4)
KspI 108 CCGCGG CCGC^GG
MaeII 1043 ACGT A^CGT
MluNI 27 TGGCCA TGG^CCA
MscI 27 TGGCCA TGG^CCA
MseI 1133 TTAA T^TAA
PstI 388 CTGCAG CTGCA^G
RsrII 479 CGGACCG CG^G[AT]CCG
SacII 108 CCGCGG CCGC^GG
SbfI 387 CCTGCAGG CCTGCA^GG
SfcI 388 CTGCAG C^T[GA][CT]AG
Sse8387I 387 CCTGCAGG CCTGCA^GG
Tru9I 1133 TTAA T^TAA
XbaI 1293 TCTAGA T^CTAGA
XmnI 492 GAATTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 25 enzymes) #
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AccII 109 CGCG CG^CG
552 CGCG
AcsI 492 GAATTC [GA]^AATT[CT]
1219 GAATTT
AlwNI 982 CAGCAACTG CAG[ACGT][ACGT][ACGT]^CTG
1011 CAGCAGCTG
ApoI 492 GAATTC [GA]^AATT[CT]
1219 GAATTT
BsaWI 482 ACCGGA [AT]^CCGG[AT]
719 TCCGGT
BseRI 469 GAGGAG GAGGAG(10/8)
1318 GAGGAG
BsgI 787 CTGCAC GTGCAG(16/14)
997 GTGCAG
BsmI 582 GAATGC GAATGC(1/-1)
224 GCATTC
BspHI 452 TCATGA T^CATGA
488 TCATGA
BstUI 109 CGCG CG^CG
552 CGCG
BstYI 669 GGATCT [GA]^GATC[CT]
1127 AGATCT
DraII 112 GGGGCCT [GA]G^G[ACGT]CC[CT]
614 AGGCCCT
EcoO109I 112 GGGGCCT [GA]G^G[ACGT]CC[CT]
614 AGGCCCT
HinfI 137 GAATC G^A[ACGT]TC
1032 GATTC
MflI 669 GGATCT [GA]^GATC[CT]
1127 AGATCT
MslI 364 CACGAGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
531 CACGGTGGTG
MvnI 109 CGCG CG^CG
552 CGCG
NcoI 273 CCATGG C^CATGG
896 CCATGG
RcaI 452 TCATGA T^CATGA
488 TCATGA
SacI 245 GAGCTC GAGCT^C
862 GAGCTC
SmaI 726 CCCGGG CCC^GGG
1073 CCCGGG
TfiI 137 GAATC G^A[AT]TC
1032 GATTC
XhoII 669 GGATCT [GA]^GATC[CT]
1127 AGATCT
XmaCI 726 CCCGGG C^CCGGG
1073 CCCGGG
XmaI 726 CCCGGG C^CCGGG
1073 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BanII BbvI BcnI
BfaI BglI BmyI BsaJI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DsaI EaeI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HpaII HphI ItaI
MaeI MaeIII MboI MboII MnlI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PflMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI