Restriction map for
pFN21AE1939
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# NO CUT ENZYMES ( 127 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI Alw44I
ApaI ApaLI ApoI AscI AseI
AsnI Asp718I AspI AvrII BbeI
BbrPI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BsgI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstUI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraIII EcoO65I EcoRI
EcoT22I FauI FbaI FseI HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MaeI MamI MfeI MluI
MroI MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
Tsp509I Tth111I TthHB8I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 829 GTCTAC GT^[AC][GT]AC
AfaI 574 GTAC GT^AC
AflIII 506 ACATGT A^C[GA][CT]GT
AhdI 965 GACCCTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 808 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1275 AGCGCT AGC^GCT
AspEI 965 GACCCTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1102 GTGCTC G[AT]GC[AT]^C
AvaI 707 CTCGGG C^[CT]CG[GA]G
AviII 353 TGCGCA TGC^GCA
BamHI 200 GGATCC G^GATCC
BbvCI 276 GCTGAGG CCTCAGC(-5/-2)
BcgI 479 GCAGTGACATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglI 703 GCCCCTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiEI 213 CGGCCG CG[GA][CT]^CG
BsiHKAI 1102 GTGCTC G[AT]GC[AT]^C
BsoBI 707 CTCGGG C^[CT]CG[GA]G
BspLU11I 506 ACATGT A^CATGT
BspMI 341 GCAGGT ACCTGC(4/8)
BsrBI 1039 CCGCTC CCGCTC(-3/-3)
BsrDI 41 CATTGC GCAATG(2/0)
BstAPI 584 GCAGGATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 29 CCATACTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 808 CCTGAGG CC^T[ACGT]AGG
DrdI 948 GACCACCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 213 CGGCCG C^GGCCG
Eam1105I 965 GACCCTTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 538 CTCTTC CTCTTC(1/4)
EclXI 213 CGGCCG C^GGCCG
Eco47III 1275 AGCGCT AGC^GCT
Eco52I 213 CGGCCG C^GGCCG
Eco81I 808 CCTGAGG CC^T[ACGT]AGG
EcoRV 890 GATATC GAT^ATC
FspI 353 TGCGCA TGC^GCA
HincII 418 GTCAAC GT[CT]^[GA]AC
HindII 418 GTCAAC GT[CT]^[GA]AC
Ksp632I 538 CTCTTC CTCTTC(1/4)
NspI 506 ACATGT [GA]CATG^[CT]
PflMI 720 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsaI 574 GTAC GT^AC
ScaI 573 AGTACT AGT^ACT
SmlI 195 CTTGAG C^T[CT][GA]AG
StuI 280 AGGCCT AGG^CCT
Tsp45I 482 GTGAC ^GT[GC]AC
Van91I 720 CCAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Asp700I 534 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
748 GAAATCTTTC
AvaII 333 GGTCC G^G[AT]CC
1074 GGACC
BanI 73 GGTGCC G^G[CT][GA]CC
1417 GGTGCC
BanII 17 GAGCCC G[GA]GC[CT]^C
378 GGGCTC
BbsI 853 GTCTTC GAAGAC(2/6)
1132 GTCTTC
BpuAI 853 GTCTTC GAAGAC(2/6)
1132 GTCTTC
BseRI 321 CTCCTC GAGGAG(10/8)
1279 CTCCTC
BsmAI 515 GAGAC GTCTC(1/5)
305 GTCTC
BspHI 797 TCATGA T^CATGA
879 TCATGA
BstYI 200 GGATCC [GA]^GATC[CT]
1391 AGATCC
Cac8I 36 GCTGGC GC[ACGT]^[ACGT]GC
865 GCCAGC
DraII 4 GGGGCCT [GA]G^G[ACGT]CC[CT]
296 AGGGCCT
DsaI 216 CCGTGG C^C[GA][CT]GG
976 CCACGG
EcoNI 443 CCTCCGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
804 CCTTCCTGAGG
EcoO109I 4 GGGGCCT [GA]G^G[ACGT]CC[CT]
296 AGGGCCT
EcoT14I 8 CCTTGG C^C[AT][AT]GG
1380 CCTTGG
HaeII 221 GGCGCT [GA]GCGC^[CT]
1275 AGCGCT
MflI 200 GGATCC [GA]^GATC[CT]
1391 AGATCC
MseI 740 TTAA T^TAA
1298 TTAA
MslI 250 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1152 CACTAATGTG
MspA1I 252 CAGCTG C[AC]G^C[GT]G
471 CAGCTG
PvuII 252 CAGCTG CAG^CTG
471 CAGCTG
RcaI 797 TCATGA T^CATGA
879 TCATGA
StyI 8 CCTTGG C^C[AT][AT]GG
1380 CCTTGG
TfiI 1062 GAATC G^A[AT]TC
1237 GAATC
Tru9I 740 TTAA T^TAA
1298 TTAA
XhoII 200 GGATCC [GA]^GATC[CT]
1391 AGATCC
XmnI 534 GAACCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
748 GAAATCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AluI AlwI AlwNI BalI
BbvI BcnI BmyI BpmI BsaJI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstF5I BstNI CfoI Cfr13I
DdeI DpnII EaeI EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MluNI MnlI
MscI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
Sau3AI Sau96I ScrFI SfaNI SfcI
TseI TspRI XcmI