Restriction map for
pFN21AE2028
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# NO CUT ENZYMES ( 130 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflIII AgeI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp718I AspI BalI
BamHI BanII BbeI BbrPI BbvCI
BcgI BlpI Bpu1102I BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsiEI
BsiWI BsmBI BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraIII DsaI EaeI
EagI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV FauI FseI
Hin1I HpaI KasI KpnI KspI
MaeII MamI MluI MluNI MroI
MscI MspA1I MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI StuI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 82 GTCGAC GT^[AC][GT]AC
AflII 1413 CTTAAG C^TTAAG
AhdI 206 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp700I 1275 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 206 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 1771 GAGCAC G[AT]GC[AT]^C
AvaI 4 CCCGAG C^[CT]CG[GA]G
AviII 149 TGCGCA TGC^GCA
AvrII 1212 CCTAGG C^CTAGG
BanI 1530 GGCACC G^G[CT][GA]CC
BbsI 547 GAAGAC GAAGAC(2/6)
BfrI 1413 CTTAAG C^TTAAG
BglI 31 GCCTCTCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 738 AGATCT A^GATCT
BlnI 1212 CCTAGG C^CTAGG
BpuAI 547 GAAGAC GAAGAC(2/6)
BsaI 322 GAGACC GGTCTC(1/5)
BsaJI 1212 CCTAGG C^C[ACGT][ACGT]GG
BsgI 64 CTGCAC GTGCAG(16/14)
BsiHKAI 1771 GAGCAC G[AT]GC[AT]^C
BsoBI 4 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1571 TGTACA T^GTACA
BsrGI 1571 TGTACA T^GTACA
BstXI 1091 CCAAACTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraII 173 AGGACCT [GA]G^G[ACGT]CC[CT]
DrdI 772 GACCTATTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 206 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 173 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 1053 GAATTC G^AATTC
EcoT14I 1212 CCTAGG C^C[AT][AT]GG
EcoT22I 1786 ATGCAT ATGCA^T
FspI 149 TGCGCA TGC^GCA
HaeII 1662 GGCGCT [GA]GCGC^[CT]
HincII 82 GTCGAC GT[CT]^[GA]AC
HindII 82 GTCGAC GT[CT]^[GA]AC
HindIII 1226 AAGCTT A^AGCTT
MfeI 1495 CAATTG C^AATTG
MslI 1018 CATTTTAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1495 CAATTG C^AATTG
NlaIV 1530 GGCACC GG[ACGT]^[ACGT]CC
NsiI 1786 ATGCAT ATGCA^T
NspI 1784 GCATGC [GA]CATG^[CT]
PleI 1389 GACTC GAGTC(4/5)
PpuMI 173 AGGACCT [GA]G^G[AT]CC[CT]
SalI 82 GTCGAC G^TCGAC
SfaNI 1788 GCATC GCATC(5/9)
SmlI 1413 CTTAAG C^T[CT][GA]AG
SphI 1784 GCATGC GCATG^C
SspBI 1571 TGTACA T^GTACA
StyI 1212 CCTAGG C^C[AT][AT]GG
SwaI 1448 ATTTAAAT ATTT^AAAT
TaqI 83 TCGA T^CGA
TthHB8I 83 TCGA T^CGA
XmnI 1275 GAAGGATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AciI 989 GCGG CCGC(-3/-1)
1620 GCGG
AfaI 969 GTAC GT^AC
1572 GTAC
BciVI 15 GGATAC GTATCC(6/5)
165 GGATAC
BclI 197 TGATCA T^GATCA
1746 TGATCA
BcnI 107 CCGGG CC^[GC]GG
1703 CCCGG
BfaI 916 CTAG C^TAG
1213 CTAG
BpmI 346 CTCCAG CTGGAG(16/14)
1522 CTCCAG
BsrDI 879 CATTGC GCAATG(2/0)
1801 GCAATG
DraI 1297 TTTAAA TTT^AAA
1449 TTTAAA
FbaI 197 TGATCA T^GATCA
1746 TGATCA
HphI 1003 GGTGA GGTGA(8/7)
1088 TCACC
MaeI 916 CTAG C^TAG
1213 CTAG
NciI 107 CCGGG CC^[GC]GG
1703 CCCGG
RsaI 969 GTAC GT^AC
1572 GTAC
#########################################
# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AcsI AluI AlwI ApoI AvaII
BbvI BmyI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
ItaI Ksp632I MaeIII MboI MboII
MflI MnlI MseI MspI MvaI
MwoI NdeII NlaIII PstI Sau3AI
Sau96I ScrFI SfcI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII