Restriction map for
pFN21AE2030
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# NO CUT ENZYMES ( 139 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BcgI BciVI
BclI BcnI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaHI
BsaI BseAI BseRI BsgI BsiEI
BsiWI BsmBI BsmI BsoBI BspDI
BspEI BspHI BspLU11I BsrFI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I Bsu36I CelII Cfr10I
ClaI CpoI DraIII EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI FbaI FseI
FspI HaeII Hin1I HpaI KasI
KpnI KspI MaeII MluI MluNI
MroI MscI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspI SwaI Tth111I
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 481 GTCTAC GT^[AC][GT]AC
AcsI 1699 GAATTT [GA]^AATT[CT]
ApoI 1699 GAATTT [GA]^AATT[CT]
AvaII 1000 GGACC G^G[AT]CC
BanI 233 GGTGCC G^G[CT][GA]CC
BanII 289 GAGCCC G[GA]GC[CT]^C
BbvCI 1384 GCTGAGG CCTCAGC(-5/-2)
BsaBI 877 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 1194 ACCGGA [AT]^CCGG[AT]
BsmFI 817 GGGAC GGGAC(10/14)
BspMI 1002 ACCTGC ACCTGC(4/8)
BsrBI 10 GAGCGG CCGCTC(-3/-3)
BssSI 98 CTCGTG CACGAG(-5/-1)
CfoI 24 GCGC GCG^C
DraI 739 TTTAAA TTT^AAA
DraII 263 AGGCCCT [GA]G^G[ACGT]CC[CT]
DrdI 1836 GACATCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1684 CCGTGG C^C[GA][CT]GG
EarI 286 GAAGAG CTCTTC(1/4)
EcoO109I 263 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoRV 962 GATATC GAT^ATC
EcoT22I 1213 ATGCAT ATGCA^T
FauI 407 GCGGG CCCGC(4/6)
HhaI 24 GCGC GCG^C
HinP1I 24 GCGC G^CGC
HincII 1321 GTTGAC GT[CT]^[GA]AC
HindII 1321 GTTGAC GT[CT]^[GA]AC
Ksp632I 286 GAAGAG CTCTTC(1/4)
MamI 877 GATTTATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1301 CAATTG C^AATTG
MspA1I 1084 CAGCTG C[AC]G^C[GT]G
MunI 1301 CAATTG C^AATTG
NsiI 1213 ATGCAT ATGCA^T
PvuII 1084 CAGCTG CAG^CTG
SapI 286 GAAGAGC GCTCTTC(1/4)
StuI 1388 AGGCCT AGG^CCT
XbaI 426 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 25 CGCG CG^CG
406 CGCG
AlwI 346 GATCC GGATC(4/5)
781 GATCC
AlwNI 354 CAGCGACTG CAG[ACGT][ACGT][ACGT]^CTG
1408 CAGGCACTG
BpmI 351 CTCCAG CTGGAG(16/14)
1766 CTCCAG
BsmAI 717 GAGAC GTCTC(1/5)
1834 GAGAC
Bsp1407I 807 TGTACA T^GTACA
1574 TGTACA
BsrDI 1188 CATTGC GCAATG(2/0)
1561 CATTGC
BsrGI 807 TGTACA T^GTACA
1574 TGTACA
BssKI 111 CCAGG ^CC[ACGT]GG
441 CCTGG
BstNI 111 CCAGG CC^[AT]GG
441 CCTGG
BstUI 25 CGCG CG^CG
406 CGCG
BstYI 345 AGATCC [GA]^GATC[CT]
780 AGATCC
Cfr13I 264 GGCCC G^G[ACGT]CC
1000 GGACC
EcoRII 111 CCAGG ^CC[AT]GG
441 CCTGG
HaeIII 264 GGCC GG^CC
1389 GGCC
HapII 30 CCGG C^CGG
1195 CCGG
HgaI 819 GACGC GACGC(5/10)
26 GCGTC
HpaII 30 CCGG C^CGG
1195 CCGG
HphI 1582 GGTGA GGTGA(8/7)
1678 TCACC
MflI 345 AGATCC [GA]^GATC[CT]
780 AGATCC
MspI 30 CCGG C^CGG
1195 CCGG
MvaI 111 CCAGG CC^[AT]GG
441 CCTGG
MvnI 25 CGCG CG^CG
406 CGCG
NlaIV 233 GGTGCC GG[ACGT]^[ACGT]CC
1332 GGAACC
PflMI 353 CCAGCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1053 CCAAGCGATGG
PstI 1362 CTGCAG CTGCA^G
1721 CTGCAG
Sau96I 264 GGCCC G^G[ACGT]CC
1000 GGACC
ScrFI 111 CCAGG CC^[ACGT]GG
441 CCTGG
SmlI 985 CTTGAG C^T[CT][GA]AG
1755 CTCAAG
SspBI 807 TGTACA T^GTACA
1574 TGTACA
TspRI 911 CAGTG CA[GC]TG[ACGT][ACGT]^
1412 CACTG
Van91I 353 CCAGCGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1053 CCAAGCGATGG
XcmI 1506 CCACACAGCCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1775 CCAGACCTTCCTTGG
XhoII 345 AGATCC [GA]^GATC[CT]
780 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AciI AfaI AluI AspHI BbsI
BbvI BfaI BmyI BpuAI BsaJI
BsiHKAI BsiYI BslI Bsp1286I BsrI
BstF5I Cac8I DdeI DpnII EcoT14I
Fnu4HI FokI HindIII HinfI ItaI
MaeI MaeIII MboI MboII MnlI
MseI MslI MwoI NdeII NlaIII
NspI RsaI Sau3AI SfaNI SfcI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TthHB8I