Restriction map for
pFN21AE1954
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# NO CUT ENZYMES ( 123 enzymes) #
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AatII Acc65I AccI AclI AflII
AflIII AgeI AhdI Alw44I AlwNI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BbrPI
BbsI BbvCI BcgI BciVI BfrI
BglI BlnI BlpI Bpu1102I BpuAI
BsaAI BsaBI BsaI BsiWI BsmBI
BsmI BsoBI Bsp1407I BspDI BspLU11I
BspMI BsrFI BsrGI Bst1107I BstBI
BstEII BstPI BstZ17I CelII Cfr10I
ClaI CpoI DraII DraIII DrdI
Eam1105I EarI Eco47III EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HincII HindII HpaI KpnI Ksp632I
KspI MamI MluI MluNI MscI
NaeI NdeI NgoMIV NheI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccIII 149 TCCGGA T^CCGGA
AcyI 205 GGCGCC G[GA]^CG[CT]C
AocI 1507 CCTAAGG CC^T[ACGT]AGG
Asp700I 1215 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbeI 205 GGCGCC GGCGC^C
BclI 399 TGATCA T^GATCA
BcnI 76 CCCGG CC^[GC]GG
BfaI 854 CTAG C^TAG
BglII 220 AGATCT A^GATCT
BsaHI 205 GGCGCC G[GA]^CG[CT]C
BsaWI 149 TCCGGA [AT]^CCGG[AT]
BseAI 149 TCCGGA T^CCGGA
BseRI 19 CTCCTC GAGGAG(10/8)
BsgI 893 CTGCAC GTGCAG(16/14)
BsmAI 1353 GTCTC GTCTC(1/5)
BsmFI 35 GTCCC GGGAC(10/14)
BspEI 149 TCCGGA T^CCGGA
BsrBI 302 GAGCGG CCGCTC(-3/-3)
BssHII 25 GCGCGC G^CGCGC
BstAPI 1405 GCAAAGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 220 AGATCT [GA]^GATC[CT]
Bsu36I 1507 CCTAAGG CC^T[ACGT]AGG
DraI 846 TTTAAA TTT^AAA
DsaI 1142 CCATGG C^C[GA][CT]GG
Eco81I 1507 CCTAAGG CC^T[ACGT]AGG
EcoT14I 1142 CCATGG C^C[AT][AT]GG
FauI 45 GCGGG CCCGC(4/6)
FbaI 399 TGATCA T^GATCA
HaeII 205 GGCGCC [GA]GCGC^[CT]
Hin1I 205 GGCGCC G[GA]^CG[CT]C
HindIII 281 AAGCTT A^AGCTT
KasI 205 GGCGCC G^GCGCC
MaeI 854 CTAG C^TAG
MaeII 696 ACGT A^CGT
MboII 1008 GAAGA GAAGA(8/7)
MfeI 482 CAATTG C^AATTG
MflI 220 AGATCT [GA]^GATC[CT]
MroI 149 TCCGGA T^CCGGA
MunI 482 CAATTG C^AATTG
NarI 205 GGCGCC GG^CGCC
NciI 76 CCCGG CC^[GC]GG
NcoI 1142 CCATGG C^CATGG
NotI 304 GCGGCCGC GC^GGCCGC
PflMI 1155 CCAAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 1351 GAGTC GAGTC(4/5)
PstI 996 CTGCAG CTGCA^G
PvuII 818 CAGCTG CAG^CTG
ScaI 615 AGTACT AGT^ACT
SfoI 205 GGCGCC GGC^GCC
SphI 260 GCATGC GCATG^C
StyI 1142 CCATGG C^C[AT][AT]GG
Van91I 1155 CCAAGTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 220 AGATCT [GA]^GATC[CT]
XmnI 1215 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AvaII 1042 GGTCC G^G[AT]CC
1522 GGTCC
BanI 183 GGTGCC G^G[CT][GA]CC
205 GGCGCC
BpmI 50 CTCCAG CTGGAG(16/14)
821 CTGGAG
BsaJI 1045 CCTCGG C^C[ACGT][ACGT]GG
1142 CCATGG
BsiEI 92 CGGCCG CG[GA][CT]^CG
305 CGGCCG
BspHI 609 TCATGA T^CATGA
951 TCATGA
BsrI 427 ACTGG ACTGG(1/-1)
271 CCAGT
BssSI 40 CTCGTG CACGAG(-5/-1)
412 CTCGTG
BstXI 321 CCAGGATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1259 CCAGGCTGCTGG
Cfr13I 1042 GGTCC G^G[ACGT]CC
1522 GGTCC
EaeI 92 CGGCCG [CT]^GGCC[GA]
305 CGGCCG
EagI 92 CGGCCG C^GGCCG
305 CGGCCG
EclXI 92 CGGCCG C^GGCCG
305 CGGCCG
Eco52I 92 CGGCCG C^GGCCG
305 CGGCCG
EcoNI 1045 CCTCGGTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1381 CCTCATATAGG
HapII 77 CCGG C^CGG
150 CCGG
HgaI 955 GACGC GACGC(5/10)
33 GCGTC
HpaII 77 CCGG C^CGG
150 CCGG
MslI 576 CACAGCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1426 CACAAAAATG
MspA1I 308 CCGCTG C[AC]G^C[GT]G
818 CAGCTG
MspI 77 CCGG C^CGG
150 CCGG
NspI 260 GCATGC [GA]CATG^[CT]
1514 ACATGC
RcaI 609 TCATGA T^CATGA
951 TCATGA
Sau96I 1042 GGTCC G^G[ACGT]CC
1522 GGTCC
SfcI 631 CTACAG C^T[GA][CT]AG
996 CTGCAG
TfiI 803 GAATC G^A[AT]TC
1310 GAATC
#########################################
# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AspHI BanII BbvI
BmyI BsiHKAI BsiYI BslI Bsp1286I
BsrDI BssKI BstF5I BstNI BstUI
Cac8I CfoI DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI MaeIII MboI
MnlI MseI MvaI MvnI MwoI
NdeII NlaIII NlaIV RsaI SacI
Sau3AI ScrFI SfaNI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I