Restriction map for
pFN21AB6995
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI Alw44I Aor51HI
ApaI ApaLI ApoI AscI Asp700I
Asp718I AspHI AspI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BciVI BclI BcnI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaBI BsaWI BseAI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrFI BsrGI BssSI Bst1107I
BstBI BstEII BstPI BstXI BstYI
BstZ17I CelII Cfr10I ClaI CpoI
DraI DraII DrdI EaeI EagI
EclXI Eco47III Eco52I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FbaI FseI FspI HaeII HpaI
KasI KpnI MaeI MamI MfeI
MflI MluI MluNI MroI MscI
MslI MunI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NlaIII
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
Psp1406I PvuI PvuII RcaI RsrII
SacI SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
StuI StyI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcyI 620 GGCGTC G[GA]^CG[CT]C
AflIII 262 ACGTGT A^C[GA][CT]GT
AhdI 348 GACGTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 485 GGATC GGATC(4/5)
AseI 421 ATTAAT AT^TAAT
AsnI 421 ATTAAT AT^TAAT
AspEI 348 GACGTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 572 GGTCC G^G[AT]CC
BanI 308 GGTGCC G^G[CT][GA]CC
BcgI 625 CGAGCCCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 160 CTGGAG CTGGAG(16/14)
BsaAI 261 TACGTG [CT]AC^GT[GA]
BsaHI 620 GGCGTC G[GA]^CG[CT]C
BsmFI 573 GTCCC GGGAC(10/14)
BspMI 316 ACCTGC ACCTGC(4/8)
BssHII 449 GCGCGC G^CGCGC
BstAPI 37 GCAGTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 449 GCGCGC GC[ACGT]^[ACGT]GC
DpnII 486 GATC ^GATC
DraIII 586 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 576 CCGCGG C^C[GA][CT]GG
Eam1105I 348 GACGTTTAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 575 CCCGC CCCGC(4/6)
HaeIII 594 GGCC GG^CC
HapII 375 CCGG C^CGG
Hin1I 620 GGCGTC G[GA]^CG[CT]C
HincII 523 GTTGAC GT[CT]^[GA]AC
HindII 523 GTTGAC GT[CT]^[GA]AC
HindIII 359 AAGCTT A^AGCTT
HpaII 375 CCGG C^CGG
KspI 576 CCGCGG CCGC^GG
MaeIII 584 GTCAC ^GT[ACGT]AC
MboI 486 GATC ^GATC
MseI 422 TTAA T^TAA
MspI 375 CCGG C^CGG
NdeII 486 GATC ^GATC
PleI 223 GAGTC GAGTC(4/5)
PshBI 421 ATTAAT AT^TAAT
PstI 504 CTGCAG CTGCA^G
SacII 576 CCGCGG CCGC^GG
Sau3AI 486 GATC ^GATC
SexAI 395 ACCTGGT A^CC[AT]GGT
SspI 418 AATATT AAT^ATT
TfiI 431 GAATC G^A[AT]TC
Tru9I 422 TTAA T^TAA
Tsp45I 584 GTCAC ^GT[GC]AC
Tsp509I 424 AATT ^AATT
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# TWO CUT ENZYMES ( 35 enzymes) #
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AccII 450 CGCG CG^CG
577 CGCG
AfaI 549 GTAC GT^AC
602 GTAC
AluI 158 AGCT AG^CT
360 AGCT
AlwNI 38 CAGTTTCTG CAG[ACGT][ACGT][ACGT]^CTG
531 CAGCCGCTG
AocI 106 CCTGAGG CC^T[ACGT]AGG
268 CCTGAGG
AvaI 495 CTCGGG C^[CT]CG[GA]G
605 CCCGAG
BanII 180 GGGCTC G[GA]GC[CT]^C
626 GAGCCC
BbsI 641 GAAGAC GAAGAC(2/6)
341 GTCTTC
BmyI 180 GGGCTC G[AGT]GC[ACT]^C
626 GAGCCC
BpuAI 641 GAAGAC GAAGAC(2/6)
341 GTCTTC
BsaI 79 GAGACC GGTCTC(1/5)
142 GAGACC
BsiYI 146 CCCCTTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
147 CCCTTGCAGGG
BslI 146 CCCCTTGCAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
147 CCCTTGCAGGG
BsoBI 495 CTCGGG C^[CT]CG[GA]G
605 CCCGAG
Bsp1286I 180 GGGCTC G[AGT]GC[ACT]^C
626 GAGCCC
BstUI 450 CGCG CG^CG
577 CGCG
Bsu36I 106 CCTGAGG CC^T[ACGT]AGG
268 CCTGAGG
CfoI 449 GCGC GCG^C
451 GCGC
Cfr13I 572 GGTCC G^G[ACGT]CC
594 GGCCC
Eco81I 106 CCTGAGG CC^T[ACGT]AGG
268 CCTGAGG
HhaI 449 GCGC GCG^C
451 GCGC
HinP1I 449 GCGC G^CGC
451 GCGC
HinfI 223 GAGTC G^A[ACGT]TC
431 GAATC
HphI 333 GGTGA GGTGA(8/7)
561 TCACC
MaeII 262 ACGT A^CGT
349 ACGT
MspA1I 534 CCGCTG C[AC]G^C[GT]G
576 CCGCGG
MvnI 450 CGCG CG^CG
577 CGCG
NlaIV 308 GGTGCC GG[ACGT]^[ACGT]CC
572 GGTCCC
RsaI 549 GTAC GT^AC
602 GTAC
SapI 22 GCTCTTC GCTCTTC(1/4)
46 GCTCTTC
Sau96I 572 GGTCC G^G[ACGT]CC
594 GGCCC
SfaNI 196 GATGC GCATC(5/9)
299 GCATC
SfcI 384 CTACAG C^T[GA][CT]AG
504 CTGCAG
TaqI 429 TCGA T^CGA
624 TCGA
TthHB8I 429 TCGA T^CGA
624 TCGA
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# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AciI BbvI BsaJI BseRI BsmAI
BsrI BssKI BstF5I BstNI DdeI
EarI EcoRII Fnu4HI FokI HgaI
ItaI Ksp632I MboII MnlI MvaI
MwoI ScrFI TseI TspRI