Restriction map for
pFN21AB7558
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AvaII AviII
AvrII BalI BbrPI BbsI BbvCI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstZ17I
Bsu36I Cac8I CelII Cfr10I Cfr13I
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT14I EcoT22I
FauI FbaI FseI FspI HaeIII
HapII HgaI HincII HindII HindIII
HpaI HpaII KpnI KspI MamI
MluI MluNI MroI MscI MspI
MvnI MwoI NaeI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacII
SalI SapI Sau96I SbfI SfcI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI StyI SwaI TspRI Tth111I
Van91I XbaI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AciI 317 GCGG CCGC(-3/-1)
AcyI 179 GGCGCC G[GA]^CG[CT]C
AspHI 65 GAGCTC G[AT]GC[AT]^C
AvaI 680 CTCGAG C^[CT]CG[GA]G
BamHI 167 GGATCC G^GATCC
BanI 179 GGCGCC G^G[CT][GA]CC
BanII 65 GAGCTC G[GA]GC[CT]^C
BbeI 179 GGCGCC GGCGC^C
BpmI 257 CTGGAG CTGGAG(16/14)
BsaHI 179 GGCGCC G[GA]^CG[CT]C
BsiHKAI 65 GAGCTC G[AT]GC[AT]^C
BsiYI 40 CCTCAACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 40 CCTCAACCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI 242 GAGACG CGTCTC(1/5)
BsoBI 680 CTCGAG C^[CT]CG[GA]G
BsrDI 333 CATTGC GCAATG(2/0)
BstF5I 131 CATCC GGATG(2/0)
CfoI 180 GCGC GCG^C
DsaI 162 CCACGG C^C[GA][CT]GG
EarI 769 GAAGAG CTCTTC(1/4)
FokI 131 CATCC GGATG(9/13)
HaeII 179 GGCGCC [GA]GCGC^[CT]
HhaI 180 GCGC GCG^C
Hin1I 179 GGCGCC G[GA]^CG[CT]C
HinP1I 180 GCGC G^CGC
KasI 179 GGCGCC G^GCGCC
Ksp632I 769 GAAGAG CTCTTC(1/4)
MaeII 25 ACGT A^CGT
MfeI 637 CAATTG C^AATTG
MslI 276 CACTTCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 315 CAGCGG C[AC]G^C[GT]G
MunI 637 CAATTG C^AATTG
NarI 179 GGCGCC GG^CGCC
NlaIII 405 CATG CATG^
PaeR7I 680 CTCGAG C^TCGAG
SacI 65 GAGCTC GAGCT^C
ScaI 421 AGTACT AGT^ACT
SexAI 45 ACCTGGT A^CC[AT]GGT
SfaNI 514 GCATC GCATC(5/9)
SfoI 179 GGCGCC GGC^GCC
SmlI 680 CTCGAG C^T[CT][GA]AG
TaqI 681 TCGA T^CGA
TfiI 7 GATTC G^A[AT]TC
TthHB8I 681 TCGA T^CGA
XcmI 488 CCAGCAATCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 680 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 11 enzymes) #
###################################
BfaI 172 CTAG C^TAG
311 CTAG
BmyI 65 GAGCTC G[AGT]GC[ACT]^C
484 GTGCCC
BsaJI 122 CCCAGG C^C[ACGT][ACGT]GG
162 CCACGG
BseRI 147 CTCCTC GAGGAG(10/8)
115 GAGGAG
BsmAI 31 GAGAC GTCTC(1/5)
242 GAGAC
BsmFI 387 GGGAC GGGAC(10/14)
120 GTCCC
Bsp1286I 65 GAGCTC G[AGT]GC[ACT]^C
484 GTGCCC
HphI 582 GGTGA GGTGA(8/7)
605 TCACC
MaeI 172 CTAG C^TAG
311 CTAG
MaeIII 274 GTCAC ^GT[ACGT]AC
598 GTGAC
Tsp45I 274 GTCAC ^GT[GC]AC
598 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 31 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
BbvI BssKI BstNI BstYI DdeI
DpnII EcoRII Fnu4HI HinfI ItaI
MboI MboII MflI MnlI MseI
MvaI NdeII NlaIV PleI RsaI
Sau3AI ScrFI Tru9I TseI Tsp509I
XhoII