Restriction map for
pFN21AB7696
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI Bpu1102I BpuAI
BsaBI BsiWI Bsp1407I BspDI BspHI
BspLU11I BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraIII
Eam1105I Eco47III Eco81I EcoNI EcoO65I
EcoRI EcoRV FbaI FseI FspI
HpaI KpnI KspI MaeI MamI
MluI MslI NcoI NdeI NheI
NotI NruI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacI SacII SalI SbfI
SfuI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tsp45I Tth111I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AccIII 535 TCCGGA T^CCGGA
AflIII 338 ACACGT A^C[GA][CT]GT
AspHI 777 GTGCTC G[AT]GC[AT]^C
AvaI 195 CCCGGG C^[CT]CG[GA]G
BalI 174 TGGCCA TGG^CCA
BamHI 507 GGATCC G^GATCC
BpmI 744 CTGGAG CTGGAG(16/14)
BsaI 979 GGTCTC GGTCTC(1/5)
BseAI 535 TCCGGA T^CCGGA
BsiHKAI 777 GTGCTC G[AT]GC[AT]^C
BsmFI 362 GTCCC GGGAC(10/14)
BsmI 765 GAATGC GAATGC(1/-1)
BsoBI 195 CCCGGG C^[CT]CG[GA]G
BspEI 535 TCCGGA T^CCGGA
BspMI 704 ACCTGC ACCTGC(4/8)
BsrDI 4 GCAATG GCAATG(2/0)
BssHII 558 GCGCGC G^CGCGC
DraII 137 GGGGCCC [GA]G^G[ACGT]CC[CT]
DrdI 847 GACCGGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 480 CCACGG C^C[GA][CT]GG
EagI 167 CGGCCG C^GGCCG
EarI 658 CTCTTC CTCTTC(1/4)
EclXI 167 CGGCCG C^GGCCG
Eco52I 167 CGGCCG C^GGCCG
EcoO109I 137 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 132 CCAAGG C^C[AT][AT]GG
EcoT22I 597 ATGCAT ATGCA^T
HgaI 123 GACGC GACGC(5/10)
HincII 919 GTTGAC GT[CT]^[GA]AC
HindII 919 GTTGAC GT[CT]^[GA]AC
Ksp632I 658 CTCTTC CTCTTC(1/4)
MaeIII 1197 GTTAC ^GT[ACGT]AC
MfeI 1138 CAATTG C^AATTG
MluNI 174 TGGCCA TGG^CCA
MroI 535 TCCGGA T^CCGGA
MscI 174 TGGCCA TGG^CCA
MseI 1217 TTAA T^TAA
MspA1I 936 CAGCTG C[AC]G^C[GT]G
MunI 1138 CAATTG C^AATTG
NaeI 817 GCCGGC GCC^GGC
NgoMIV 817 GCCGGC G^CCGGC
NsiI 597 ATGCAT ATGCA^T
PleI 922 GACTC GAGTC(4/5)
PstI 706 CTGCAG CTGCA^G
PvuII 936 CAGCTG CAG^CTG
SapI 657 GCTCTTC GCTCTTC(1/4)
ScaI 395 AGTACT AGT^ACT
SexAI 719 ACCAGGT A^CC[AT]GGT
SfcI 706 CTGCAG C^T[GA][CT]AG
SmaI 195 CCCGGG CCC^GGG
StuI 65 AGGCCT AGG^CCT
StyI 132 CCAAGG C^C[AT][AT]GG
TfiI 1076 GATTC G^A[AT]TC
Tru9I 1217 TTAA T^TAA
XmaCI 195 CCCGGG C^CCGGG
XmaI 195 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
ApaI 138 GGGCCC GGGCC^C
677 GGGCCC
AvaII 717 GGACC G^G[AT]CC
867 GGACC
BbeI 75 GGCGCC GGCGC^C
103 GGCGCC
BsaWI 353 ACCGGA [AT]^CCGG[AT]
535 TCCGGA
BseRI 1119 CTCCTC GAGGAG(10/8)
1122 CTCCTC
BsgI 913 CTGCAC GTGCAG(16/14)
445 GTGCAG
BsiEI 167 CGGCCG CG[GA][CT]^CG
846 CGACCG
BsmBI 1116 CGTCTC CGTCTC(1/5)
432 GAGACG
BsrBI 802 CCGCTC CCGCTC(-3/-3)
258 GAGCGG
BsrFI 169 GCCGGT [GA]^CCGG[CT]
817 GCCGGC
BstAPI 383 GCAACCAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
590 GCAGCCAATGC
BstYI 507 GGATCC [GA]^GATC[CT]
878 GGATCT
Cfr10I 169 GCCGGT [GA]^CCGG[CT]
817 GCCGGC
HindIII 1005 AAGCTT A^AGCTT
1084 AAGCTT
HinfI 922 GACTC G^A[ACGT]TC
1076 GATTC
HphI 740 TCACC GGTGA(8/7)
962 TCACC
KasI 75 GGCGCC G^GCGCC
103 GGCGCC
MflI 507 GGATCC [GA]^GATC[CT]
878 GGATCT
NarI 75 GGCGCC GG^CGCC
103 GGCGCC
NspI 599 GCATGC [GA]CATG^[CT]
825 GCATGC
SfoI 75 GGCGCC GGC^GCC
103 GGCGCC
SmlI 904 CTCAAG C^T[CT][GA]AG
943 CTCAAG
SphI 599 GCATGC GCATG^C
825 GCATGC
Tsp509I 1139 AATT ^AATT
1236 AATT
XcmI 177 CCAGGGCGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
608 CCAACAACATCATGG
XhoII 507 GGATCC [GA]^GATC[CT]
878 GGATCT
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# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI BanI BanII BbrPI BbvI
BcnI BglI BmyI BsaAI BsaHI
BsaJI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HpaII ItaI MaeII MboI
MboII MnlI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PmaCI PmlI RsaI Sau3AI Sau96I
ScrFI SfaNI SfiI TaqI TseI
TspRI TthHB8I