Restriction map for
pFN21AB6416
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BfrI BglI BlnI BlpI
BpmI Bpu1102I BsaAI BsaHI BsaWI
BseAI BsiEI BsiHKAI BsiWI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrDI BsrFI BsrGI
BssHII Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraII
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HapII HgaI Hin1I HincII
HindII HpaI HpaII KasI KpnI
Ksp632I KspI MaeII MfeI MluI
MluNI MroI MscI MspA1I MspI
MunI MvnI NaeI NarI NciI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TspRI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 44 enzymes) #
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AlwI 723 GGATC GGATC(4/5)
AvaII 62 GGTCC G^G[AT]CC
BanI 838 GGTGCC G^G[CT][GA]CC
BanII 737 GAGCCC G[GA]GC[CT]^C
BbsI 596 GTCTTC GAAGAC(2/6)
BfaI 157 CTAG C^TAG
BglII 534 AGATCT A^GATCT
BmyI 737 GAGCCC G[AGT]GC[ACT]^C
BpuAI 596 GTCTTC GAAGAC(2/6)
BsaBI 718 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 921 GAGACC GGTCTC(1/5)
BseRI 662 GAGGAG GAGGAG(10/8)
BsgI 683 GTGCAG GTGCAG(16/14)
BsmBI 304 GAGACG CGTCTC(1/5)
BsmFI 272 GGGAC GGGAC(10/14)
Bsp1286I 737 GAGCCC G[AGT]GC[ACT]^C
BspMI 835 GCAGGT ACCTGC(4/8)
BsrI 321 ACTGG ACTGG(1/-1)
BssKI 70 CCTGG ^CC[ACGT]GG
BstNI 70 CCTGG CC^[AT]GG
CfoI 805 GCGC GCG^C
Cfr13I 62 GGTCC G^G[ACGT]CC
DraI 885 TTTAAA TTT^AAA
DsaI 195 CCATGG C^C[GA][CT]GG
EcoRII 70 CCTGG ^CC[AT]GG
HhaI 805 GCGC GCG^C
HinP1I 805 GCGC G^CGC
HindIII 974 AAGCTT A^AGCTT
HinfI 555 GATTC G^A[ACGT]TC
MaeI 157 CTAG C^TAG
MamI 718 GATTTGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvaI 70 CCTGG CC^[AT]GG
NcoI 195 CCATGG C^CATGG
PflMI 741 CCATCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 893 CTGCAG CTGCA^G
Sau96I 62 GGTCC G^G[ACGT]CC
ScrFI 70 CCTGG CC^[ACGT]GG
SfcI 893 CTGCAG C^T[GA][CT]AG
StuI 951 AGGCCT AGG^CCT
TaqI 26 TCGA T^CGA
TfiI 555 GATTC G^A[AT]TC
TthHB8I 26 TCGA T^CGA
Van91I 741 CCATCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 842 CCATGATGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AciI 4 GCGG CCGC(-3/-1)
818 GCGG
AfaI 231 GTAC GT^AC
583 GTAC
AluI 86 AGCT AG^CT
975 AGCT
BsaJI 195 CCATGG C^C[ACGT][ACGT]GG
937 CCAAGG
BssSI 918 CACGAG CACGAG(-5/-1)
125 CTCGTG
BstYI 534 AGATCT [GA]^GATC[CT]
723 GGATCT
Cac8I 382 GCCAGC GC[ACGT]^[ACGT]GC
891 GCCTGC
EcoT14I 195 CCATGG C^C[AT][AT]GG
937 CCAAGG
HaeIII 324 GGCC GG^CC
952 GGCC
MflI 534 AGATCT [GA]^GATC[CT]
723 GGATCT
MwoI 552 GCAGATTCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
561 GCGAATTATGC
RsaI 231 GTAC GT^AC
583 GTAC
SfaNI 386 GCATC GCATC(5/9)
946 GCATC
StyI 195 CCATGG C^C[AT][AT]GG
937 CCAAGG
Tsp45I 188 GTGAC ^GT[GC]AC
790 GTCAC
XhoII 534 AGATCT [GA]^GATC[CT]
723 GGATCT
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# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AcsI ApoI Asp700I BbvI BsiYI
BslI BsmAI BstF5I DdeI DpnII
Fnu4HI FokI HphI ItaI MaeIII
MboI MboII MnlI MseI MslI
NdeII NlaIII NlaIV Sau3AI Tru9I
TseI Tsp509I XmnI