Restriction map for
pFN21AE1818
##################################
# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AflII AflIII AgeI AhdI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BalI
BamHI BbrPI BbsI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI BsmFI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstXI BstZ17I CelII ClaI CpoI
DraI DraII DraIII DsaI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoNI EcoO109I EcoO65I EcoT22I FbaI
FseI FspI HincII HindII HpaI
KpnI Ksp632I KspI MaeII MamI
MboII MluI MluNI MroI MscI
MseI MslI MspA1I MvnI NaeI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tsp45I XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 268 GTCTAC GT^[AC][GT]AC
AciI 717 GCGG CCGC(-3/-1)
Alw44I 488 GTGCAC G^TGCAC
AlwI 339 GATCC GGATC(4/5)
AlwNI 185 CAGGCACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 488 GTGCAC G^TGCAC
AspI 96 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 287 CTCGAG C^[CT]CG[GA]G
AvaII 646 GGACC G^G[AT]CC
BanII 401 GAGCTC G[GA]GC[CT]^C
BbeI 809 GGCGCC GGCGC^C
BfaI 406 CTAG C^TAG
BglII 591 AGATCT A^GATCT
BpmI 371 CTGGAG CTGGAG(16/14)
BsmAI 676 GTCTC GTCTC(1/5)
BsmI 435 GAATGC GAATGC(1/-1)
BsoBI 287 CTCGAG C^[CT]CG[GA]G
BspMI 547 ACCTGC ACCTGC(4/8)
Cac8I 352 GCAGGC GC[ACGT]^[ACGT]GC
CfoI 810 GCGC GCG^C
DrdI 647 GACCTGGCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 651 TGGCCG [CT]^GGCC[GA]
EcoRI 530 GAATTC G^AATTC
EcoRV 557 GATATC GAT^ATC
EcoT14I 208 CCTTGG C^C[AT][AT]GG
FauI 717 GCGGG CCCGC(4/6)
HaeII 809 GGCGCC [GA]GCGC^[CT]
HgaI 569 GCGTC GACGC(5/10)
HhaI 810 GCGC GCG^C
HinP1I 810 GCGC G^CGC
HindIII 387 AAGCTT A^AGCTT
KasI 809 GGCGCC G^GCGCC
MaeI 406 CTAG C^TAG
MfeI 743 CAATTG C^AATTG
MunI 743 CAATTG C^AATTG
NarI 809 GGCGCC GG^CGCC
NspI 57 ACATGC [GA]CATG^[CT]
PaeR7I 287 CTCGAG C^TCGAG
PflFI 96 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 457 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 658 CTGCAG CTGCA^G
SacI 401 GAGCTC GAGCT^C
SfaNI 714 GATGC GCATC(5/9)
SfcI 658 CTGCAG C^T[GA][CT]AG
SfoI 809 GGCGCC GGC^GCC
StuI 617 AGGCCT AGG^CCT
StyI 208 CCTTGG C^C[AT][AT]GG
TaqI 288 TCGA T^CGA
Tth111I 96 GACCTCGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 288 TCGA T^CGA
Van91I 457 CCAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 691 CCAAAGCCCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 287 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AcsI 481 AAATTC [GA]^AATT[CT]
530 GAATTC
AcyI 568 GGCGTC G[GA]^CG[CT]C
809 GGCGCC
AfaI 116 GTAC GT^AC
295 GTAC
AocI 393 CCTGAGG CC^T[ACGT]AGG
613 CCTGAGG
ApoI 481 AAATTC [GA]^AATT[CT]
530 GAATTC
AspHI 401 GAGCTC G[AT]GC[AT]^C
488 GTGCAC
BanI 376 GGTGCC G^G[CT][GA]CC
809 GGCGCC
BsaHI 568 GGCGTC G[GA]^CG[CT]C
809 GGCGCC
BseRI 535 CTCCTC GAGGAG(10/8)
253 GAGGAG
BsiHKAI 401 GAGCTC G[AT]GC[AT]^C
488 GTGCAC
BsrFI 307 GCCGGT [GA]^CCGG[CT]
812 GCCGGT
BsrI 629 CCAGT ACTGG(1/-1)
638 CCAGT
BstF5I 150 GGATG GGATG(2/0)
713 GGATG
BstYI 338 AGATCC [GA]^GATC[CT]
591 AGATCT
Bsu36I 393 CCTGAGG CC^T[ACGT]AGG
613 CCTGAGG
Cfr10I 307 GCCGGT [GA]^CCGG[CT]
812 GCCGGT
DpnII 339 GATC ^GATC
592 GATC
Eco81I 393 CCTGAGG CC^T[ACGT]AGG
613 CCTGAGG
FokI 150 GGATG GGATG(9/13)
713 GGATG
HapII 308 CCGG C^CGG
813 CCGG
Hin1I 568 GGCGTC G[GA]^CG[CT]C
809 GGCGCC
HpaII 308 CCGG C^CGG
813 CCGG
HphI 310 GGTGA GGTGA(8/7)
705 GGTGA
MaeIII 47 GTAAC ^GT[ACGT]AC
221 GTAAC
MboI 339 GATC ^GATC
592 GATC
MflI 338 AGATCC [GA]^GATC[CT]
591 AGATCT
MspI 308 CCGG C^CGG
813 CCGG
NdeII 339 GATC ^GATC
592 GATC
PleI 181 GACTC GAGTC(4/5)
266 GAGTC
RsaI 116 GTAC GT^AC
295 GTAC
Sau3AI 339 GATC ^GATC
592 GATC
SmlI 131 CTCAAG C^T[CT][GA]AG
287 CTCGAG
XhoII 338 AGATCC [GA]^GATC[CT]
591 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 27 enzymes) #
#########################################
AluI BbvI BmyI BsaJI BsiYI
BslI Bsp1286I BssKI BstNI Cfr13I
DdeI EcoRII Fnu4HI HaeIII HinfI
ItaI MnlI MvaI MwoI NlaIII
NlaIV Sau96I ScrFI TfiI TseI
Tsp509I TspRI