##################################
# NO CUT ENZYMES (  182 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AcyI        AflII       AflIII      AgeI        AhdI        
Alw44I      AlwNI       AocI        Aor51HI     ApaI        
ApaLI       AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BcgI        
BciVI       BclI        BcnI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmBI       BsoBI       Bsp1286I    BspEI       BspLU11I    
BspMI       BsrBI       BsrFI       BssHII      BssSI       
Bst1107I    BstAPI      BstBI       BstEII      BstPI       
BstUI       BstXI       BstZ17I     Bsu36I      CelII       
CfoI        Cfr10I      CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
FauI        FbaI        FseI        FspI        HaeII       
HapII       HgaI        HhaI        Hin1I       HinP1I      
HincII      HindII      HindIII     HpaI        HpaII       
HphI        KasI        KpnI        KspI        MaeI        
MaeIII      MamI        MfeI        MluI        MluNI       
MroI        MscI        MslI        MspA1I      MspI        
MunI        MvnI        NaeI        NarI        NciI        
NcoI        NgoMIV      NheI        NotI        NruI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       PstI        PvuI        PvuII       
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SwaI        Tsp45I      
Tth111I     Van91I      XbaI        XcmI        XhoI        
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AclI          452  AACGTT               AA^CGTT
AluI          569  AGCT                 AG^CT
AlwI          388  GATCC                GGATC(4/5)
BbvI           51  GCTGC                GCAGC(8/12)
BpmI          656  CTCCAG               CTGGAG(16/14)
BsmFI         638  GTCCC                GGGAC(10/14)
Bsp1407I      310  TGTACA               T^GTACA
BspDI         275  ATCGAT               AT^CGAT
BspHI         197  TCATGA               T^CATGA
BsrDI         347  CATTGC               GCAATG(2/0)
BsrGI         310  TGTACA               T^GTACA
BsrI          501  ACTGG                ACTGG(1/-1)
BstYI         387  AGATCC               [GA]^GATC[CT]
Cac8I          47  GCCTGC               GC[ACGT]^[ACGT]GC
ClaI          275  ATCGAT               AT^CGAT
DpnII         388  GATC                 ^GATC
EarI          292  GAAGAG               CTCTTC(1/4)
EcoT14I       608  CCAAGG               C^C[AT][AT]GG
EcoT22I       148  ATGCAT               ATGCA^T
Ksp632I       292  GAAGAG               CTCTTC(1/4)
MaeII         453  ACGT                 A^CGT
MboI          388  GATC                 ^GATC
MflI          387  AGATCC               [GA]^GATC[CT]
NdeI          884  CATATG               CA^TATG
NdeII         388  GATC                 ^GATC
NsiI          148  ATGCAT               ATGCA^T
PleI          594  GACTC                GAGTC(4/5)
Psp1406I      452  AACGTT               AA^CGTT
RcaI          197  TCATGA               T^CATGA
Sau3AI        388  GATC                 ^GATC
SexAI         415  ACCAGGT              A^CC[AT]GGT
SfaNI         470  GATGC                GCATC(5/9)
SspBI         310  TGTACA               T^GTACA
SspI          217  AATATT               AAT^ATT
StuI          834  AGGCCT               AGG^CCT
StyI          608  CCAAGG               C^C[AT][AT]GG
TseI           51  GCTGC                G^C[AT]GC
XhoII         387  AGATCC               [GA]^GATC[CT]


###################################
# TWO CUT ENZYMES (   11 enzymes) #
###################################

Asp700I       537  GAATGGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              690  GAACAGATTC          
AvaII         532  GGTCC                G^G[AT]CC
              637  GGTCC               
BsaJI         474  CCTGGG               C^C[ACGT][ACGT]GG
              608  CCAAGG              
BsmAI         496  GAGAC                GTCTC(1/5)
              650  GTCTC               
DdeI           89  CTGAG                C^T[ACGT]AG
              790  CTGAG               
Fnu4HI         51  GCTGC                GC^[ACGT]GC
              725  GCCGC               
HaeIII        181  GGCC                 GG^CC
              835  GGCC                
ItaI           51  GCTGC                GC^[ACGT]GC
              725  GCCGC               
MwoI           28  GCACTTTGGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              163  GCATTCAAGGC         
NspI           24  ACATGC               [GA]CATG^[CT]
              796  GCATGT              
XmnI          537  GAATGGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              690  GAACAGATTC          


#########################################
# 3 OR MORE CUT ENZYMES (   27 enzymes) #
#########################################

AciI        AcsI        AfaI        ApoI        BsmI        
BssKI       BstF5I      BstNI       Cfr13I      EcoRII      
FokI        HinfI       MboII       MnlI        MseI        
MvaI        NlaIII      NlaIV       RsaI        Sau96I      
ScrFI       TaqI        TfiI        Tru9I       Tsp509I     
TspRI       TthHB8I