##################################
# NO CUT ENZYMES (  220 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       ApoI        AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AspHI       AspI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbsI        BbvCI       BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BpuAI       BsaBI       BsaHI       BsaI        
BsaWI       BseAI       BseRI       BsgI        BsiEI       
BsiHKAI     BsiWI       BsmFI       Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BspMI       
BsrDI       BsrFI       BsrGI       BsrI        BssHII      
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
Cfr13I      ClaI        CpoI        DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EarI        EclXI       Eco47III    Eco52I      
Eco81I      EcoNI       EcoO109I    EcoO65I     EcoRI       
EcoRV       EcoT14I     EcoT22I     FbaI        FokI        
FseI        FspI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HincII      
HindII      HindIII     HinfI       HpaI        HpaII       
HphI        KasI        KpnI        Ksp632I     KspI        
MaeI        MaeIII      MamI        MboII       MfeI        
MflI        MluI        MluNI       MroI        MscI        
MseI        MslI        MspA1I      MspI        MunI        
MvnI        NaeI        NarI        NciI        NcoI        
NdeI        NgoMIV      NheI        NlaIII      NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PinAI       PleI        
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        PvuII       
RcaI        RsrII       SacI        SacII       SalI        
SapI        Sau96I      SbfI        ScaI        SexAI       
SfaNI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SmlI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        StyI        
SwaI        TaqI        TfiI        Tru9I       Tsp45I      
Tsp509I     Tth111I     TthHB8I     XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   27 enzymes) #
###################################

AciI            6  GCGG                 CCGC(-3/-1)
AluI          192  AGCT                 AG^CT
AlwI           15  GGATC                GGATC(4/5)
AvaI          123  CCCGAG               C^[CT]CG[GA]G
BsaAI          49  CACGTA               [CT]AC^GT[GA]
BsaJI         124  CCGAGG               C^C[ACGT][ACGT]GG
BsiYI         202  CCAAGAGGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          202  CCAAGAGGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmBI          30  GAGACG               CGTCTC(1/5)
BsmI          184  GCATTC               GAATGC(1/-1)
BsoBI         123  CCCGAG               C^[CT]CG[GA]G
BsrBI           4  GAGCGG               CCGCTC(-3/-3)
BssKI         165  CCAGG                ^CC[ACGT]GG
BstNI         165  CCAGG                CC^[AT]GG
DpnII          16  GATC                 ^GATC
EcoRII        165  CCAGG                ^CC[AT]GG
FauI            6  GCGGG                CCCGC(4/6)
MaeII          50  ACGT                 A^CGT
MboI           16  GATC                 ^GATC
MvaI          165  CCAGG                CC^[AT]GG
NdeII          16  GATC                 ^GATC
PflMI         202  CCAAGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Sau3AI         16  GATC                 ^GATC
ScrFI         165  CCAGG                CC^[ACGT]GG
SfcI           38  CTACAG               C^T[GA][CT]AG
TspRI          95  CACTG                CA[GC]TG[ACGT][ACGT]^
Van91I        202  CCAAGAGGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG


###################################
# TWO CUT ENZYMES (    7 enzymes) #
###################################

BbvI          138  GCAGC                GCAGC(8/12)
              141  GCAGC               
BsmAI          30  GAGAC                GTCTC(1/5)
              196  GAGAC               
Cac8I           6  GCGGGC               GC[ACGT]^[ACGT]GC
              180  GCAGGC              
Fnu4HI        138  GCAGC                GC^[ACGT]GC
              141  GCAGC               
ItaI          138  GCAGC                GC^[ACGT]GC
              141  GCAGC               
MwoI          129  GCGTTCTCAGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              184  GCATTCTTAGC         
TseI          138  GCAGC                G^C[AT]GC
              141  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (    4 enzymes) #
#########################################

AfaI        DdeI        MnlI        RsaI