Restriction map for
pFN21AE1844
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# NO CUT ENZYMES ( 133 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AflIII AgeI Alw44I AocI
Aor51HI ApaLI AscI AseI AsnI
AspI AviII AvrII BbrPI BbvCI
BclI BfrI BglI BglII BlnI
BpmI BsaAI BsaBI BseAI BseRI
BsgI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrFI BsrGI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I Cfr10I ClaI CpoI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI FspI HgaI
HincII HindII HindIII HpaI HphI
KspI MamI MfeI MluI MroI
MslI MspA1I MunI MvnI NaeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 58 enzymes) #
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Acc65I 393 GGTACC G^GTACC
AcsI 340 GAATTC [GA]^AATT[CT]
AcyI 417 GGCGCC G[GA]^CG[CT]C
AfaI 394 GTAC GT^AC
AhdI 381 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 871 GGGCCC GGGCC^C
ApoI 340 GAATTC [GA]^AATT[CT]
Asp700I 774 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 393 GGTACC G^GTACC
AspEI 381 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 677 GAGCAC G[AT]GC[AT]^C
BalI 584 TGGCCA TGG^CCA
BamHI 26 GGATCC G^GATCC
BanII 871 GGGCCC G[GA]GC[CT]^C
BbeI 417 GGCGCC GGCGC^C
BbsI 460 GAAGAC GAAGAC(2/6)
BcgI 606 GCAGAAGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3 GTATCC GTATCC(6/5)
BlpI 664 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 664 GCTAAGC GC^T[ACGT]AGC
BpuAI 460 GAAGAC GAAGAC(2/6)
BsaHI 417 GGCGCC G[GA]^CG[CT]C
BsaI 1038 GAGACC GGTCTC(1/5)
BsiHKAI 677 GAGCAC G[AT]GC[AT]^C
BsmAI 1038 GAGAC GTCTC(1/5)
BsmI 753 GAATGC GAATGC(1/-1)
BsrBI 178 CCGCTC CCGCTC(-3/-3)
BsrDI 327 GCAATG GCAATG(2/0)
BstAPI 508 GCAGTGCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 237 GGATG GGATG(2/0)
BstXI 900 CCAGAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 664 GCTAAGC GC^T[ACGT]AGC
DraII 870 GGGGCCC [GA]G^G[ACGT]CC[CT]
DsaI 644 CCATGG C^C[GA][CT]GG
EaeI 584 TGGCCA [CT]^GGCC[GA]
Eam1105I 381 GACACTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 723 GAAGAG CTCTTC(1/4)
EcoO109I 870 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoRI 340 GAATTC G^AATTC
EcoT14I 644 CCATGG C^C[AT][AT]GG
FokI 237 GGATG GGATG(9/13)
Hin1I 417 GGCGCC G[GA]^CG[CT]C
KasI 417 GGCGCC G^GCGCC
KpnI 393 GGTACC GGTAC^C
Ksp632I 723 GAAGAG CTCTTC(1/4)
MluNI 584 TGGCCA TGG^CCA
MscI 584 TGGCCA TGG^CCA
MseI 761 TTAA T^TAA
NarI 417 GGCGCC GG^CGCC
NcoI 644 CCATGG C^CATGG
NdeI 745 CATATG CA^TATG
PleI 193 GAGTC GAGTC(4/5)
RsaI 394 GTAC GT^AC
SfoI 417 GGCGCC GGC^GCC
StyI 644 CCATGG C^C[AT][AT]GG
Tru9I 761 TTAA T^TAA
Tsp509I 341 AATT ^AATT
XmnI 774 GAAAGGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AlwNI 135 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
619 CAGAAGCTG
AvaI 248 CCCGAG C^[CT]CG[GA]G
947 CTCGGG
AvaII 388 GGTCC G^G[AT]CC
438 GGACC
BanI 393 GGTACC G^G[CT][GA]CC
417 GGCGCC
BcnI 39 CCCGG CC^[GC]GG
978 CCGGG
BfaI 283 CTAG C^TAG
971 CTAG
BmyI 677 GAGCAC G[AGT]GC[ACT]^C
871 GGGCCC
BsoBI 248 CCCGAG C^[CT]CG[GA]G
947 CTCGGG
Bsp1286I 677 GAGCAC G[AGT]GC[ACT]^C
871 GGGCCC
CfoI 13 GCGC GCG^C
418 GCGC
HaeII 12 GGCGCT [GA]GCGC^[CT]
417 GGCGCC
HhaI 13 GCGC GCG^C
418 GCGC
HinP1I 13 GCGC G^CGC
418 GCGC
MaeI 283 CTAG C^TAG
971 CTAG
MaeII 150 ACGT A^CGT
311 ACGT
MaeIII 217 GTCAC ^GT[ACGT]AC
379 GTGAC
NciI 39 CCCGG CC^[GC]GG
978 CCGGG
NlaIII 645 CATG CATG^
994 CATG
PstI 598 CTGCAG CTGCA^G
625 CTGCAG
SfaNI 238 GATGC GCATC(5/9)
603 GATGC
SmlI 802 CTTGAG C^T[CT][GA]AG
1006 CTCAAG
TaqI 374 TCGA T^CGA
615 TCGA
Tsp45I 217 GTCAC ^GT[GC]AC
379 GTGAC
TthHB8I 374 TCGA T^CGA
615 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 43 enzymes) #
#########################################
AciI AluI AlwI BbvI BsaJI
BsaWI BsiYI BslI BsmFI BsrI
BssKI BstNI BstYI Cac8I Cfr13I
DdeI DpnII EcoRII FauI Fnu4HI
HaeIII HapII HinfI HpaII ItaI
MboI MboII MflI MnlI MspI
MvaI MwoI NdeII NlaIV Sau3AI
Sau96I ScrFI SfcI StuI TfiI
TseI TspRI XhoII