Restriction map for
pFN21AE1873
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BanI
BanII BbeI BbvCI BciVI BclI
BfrI BglI BglII BlnI BsaBI
BsaHI BsgI BsiWI BsmBI Bsp1407I
BspDI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstZ17I Cfr10I ClaI CpoI DraI
DraII DraIII DrdI DsaI Eam1105I
Eco47III EcoNI EcoO109I EcoRI EcoRV
EcoT22I FbaI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PinAI
PmeI PpuMI PshAI PshBI Psp1406I
PvuI RcaI RsrII SacI SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI StuI
SwaI TfiI XbaI XcmI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AccI 1120 GTAGAC GT^[AC][GT]AC
AccII 57 CGCG CG^CG
AccIII 1043 TCCGGA T^CCGGA
AcsI 940 GAATTT [GA]^AATT[CT]
AflIII 323 ACGTGT A^C[GA][CT]GT
AlwNI 977 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 33 CCTGAGG CC^T[ACGT]AGG
ApoI 940 GAATTT [GA]^AATT[CT]
AspHI 66 GAGCAC G[AT]GC[AT]^C
AspI 904 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 213 CTCGAG C^[CT]CG[GA]G
AvaII 1067 GGACC G^G[AT]CC
BalI 758 TGGCCA TGG^CCA
BbrPI 322 CACGTG CAC^GTG
BcgI 231 CGACTCCTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 775 CTAG C^TAG
BlpI 118 GCTAAGC GC^T[ACGT]AGC
BmyI 66 GAGCAC G[AGT]GC[ACT]^C
Bpu1102I 118 GCTAAGC GC^T[ACGT]AGC
BsaI 1103 GAGACC GGTCTC(1/5)
BseAI 1043 TCCGGA T^CCGGA
BsiEI 59 CGGCCG CG[GA][CT]^CG
BsiHKAI 66 GAGCAC G[AT]GC[AT]^C
BsmAI 1103 GAGAC GTCTC(1/5)
BsoBI 213 CTCGAG C^[CT]CG[GA]G
Bsp1286I 66 GAGCAC G[AGT]GC[ACT]^C
BspEI 1043 TCCGGA T^CCGGA
BspMI 742 ACCTGC ACCTGC(4/8)
BsrDI 129 GCAATG GCAATG(2/0)
BssSI 256 CACGAG CACGAG(-5/-1)
BstUI 57 CGCG CG^CG
BstXI 464 CCAACCACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 33 CCTGAGG CC^T[ACGT]AGG
CelII 118 GCTAAGC GC^T[ACGT]AGC
CfoI 897 GCGC GCG^C
EagI 59 CGGCCG C^GGCCG
EclXI 59 CGGCCG C^GGCCG
Eco52I 59 CGGCCG C^GGCCG
Eco81I 33 CCTGAGG CC^T[ACGT]AGG
EcoT14I 389 CCAAGG C^C[AT][AT]GG
FauI 20 CCCGC CCCGC(4/6)
HgaI 134 GCGTC GACGC(5/10)
HhaI 897 GCGC GCG^C
HinP1I 897 GCGC G^CGC
MaeI 775 CTAG C^TAG
MluNI 758 TGGCCA TGG^CCA
MroI 1043 TCCGGA T^CCGGA
MscI 758 TGGCCA TGG^CCA
MvnI 57 CGCG CG^CG
PaeR7I 213 CTCGAG C^TCGAG
PflFI 904 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 912 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 322 CACGTG CAC^GTG
PmlI 322 CACGTG CAC^GTG
PstI 886 CTGCAG CTGCA^G
SapI 856 GAAGAGC GCTCTTC(1/4)
SfcI 886 CTGCAG C^T[GA][CT]AG
SmlI 213 CTCGAG C^T[CT][GA]AG
SspI 934 AATATT AAT^ATT
StyI 389 CCAAGG C^C[AT][AT]GG
Tsp509I 941 AATT ^AATT
Tth111I 904 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 912 CCACGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 213 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 20 enzymes) #
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BcnI 651 CCGGG CC^[GC]GG
750 CCGGG
BpmI 426 CTCCAG CTGGAG(16/14)
629 CTGGAG
BsaAI 322 CACGTG [CT]AC^GT[GA]
664 TACGTG
BsaWI 296 ACCGGA [AT]^CCGG[AT]
1043 TCCGGA
BsmI 421 GAATGC GAATGC(1/-1)
877 GCATTC
BstEII 804 GGTGACC G^GT[ACGT]ACC
921 GGTCACC
BstPI 804 GGTGACC G^GT[ACGT]ACC
921 GGTCACC
Cac8I 26 GCCTGC GC[ACGT]^[ACGT]GC
884 GCCTGC
Cfr13I 398 GGGCC G^G[ACGT]CC
1067 GGACC
EaeI 59 CGGCCG [CT]^GGCC[GA]
758 TGGCCA
EarI 508 GAAGAG CTCTTC(1/4)
856 GAAGAG
EcoO65I 804 GGTGACC G^GT[ACGT]ACC
921 GGTCACC
Ksp632I 508 GAAGAG CTCTTC(1/4)
856 GAAGAG
MaeIII 805 GTGAC ^GT[ACGT]AC
922 GTCAC
MslI 69 CACCGAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1071 CATATCCATG
NciI 651 CCGGG CC^[GC]GG
750 CCGGG
PvuII 159 CAGCTG CAG^CTG
1141 CAGCTG
Sau96I 398 GGGCC G^G[ACGT]CC
1067 GGACC
SfaNI 985 GATGC GCATC(5/9)
746 GCATC
Tsp45I 805 GTGAC ^GT[GC]AC
922 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AciI AfaI AluI AlwI BamHI
BbsI BbvI BpuAI BsaJI BseRI
BsiYI BslI BsmFI BsrI BssKI
BstF5I BstNI BstYI DdeI DpnII
EcoRII Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
ScrFI TaqI Tru9I TseI TspRI
TthHB8I XhoII