##################################
# NO CUT ENZYMES (  203 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AviII       AvrII       
BalI        BamHI       BanI        BanII       BbeI        
BbrPI       BbvCI       BcgI        BciVI       BcnI        
BfaI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaWI       BseAI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsmBI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
Cfr13I      ClaI        CpoI        DdeI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT14I     EcoT22I     FauI        FseI        FspI        
HaeII       HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HinfI       
HpaI        HpaII       KasI        KpnI        KspI        
MaeI        MaeII       MamI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MslI        
MspA1I      MspI        MunI        MvnI        NaeI        
NarI        NciI        NcoI        NdeI        NgoMIV      
NheI        NotI        NruI        NsiI        NspI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PleI        PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        Sau96I      SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
StyI        SwaI        TaqI        TfiI        Tth111I     
TthHB8I     Van91I      XbaI        XhoI        XhoII       
XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   25 enzymes) #
###################################

AfaI          244  GTAC                 GT^AC
AlwI          645  GGATC                GGATC(4/5)
BbsI          697  GAAGAC               GAAGAC(2/6)
BbvI          410  GCAGC                GCAGC(8/12)
BclI          443  TGATCA               T^GATCA
BpuAI         697  GAAGAC               GAAGAC(2/6)
BsaI          505  GAGACC               GGTCTC(1/5)
BseRI         716  CTCCTC               GAGGAG(10/8)
BsiYI         301  CCATTCTGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI          301  CCATTCTGGGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI         573  GGGAC                GGGAC(10/14)
BspMI         292  GCAGGT               ACCTGC(4/8)
BsrI           53  CCAGT                ACTGG(1/-1)
DraI          286  TTTAAA               TTT^AAA
EarI          459  GAAGAG               CTCTTC(1/4)
FbaI          443  TGATCA               T^GATCA
Fnu4HI        410  GCAGC                GC^[ACGT]GC
ItaI          410  GCAGC                GC^[ACGT]GC
Ksp632I       459  GAAGAG               CTCTTC(1/4)
NlaIII        146  CATG                 CATG^
RsaI          244  GTAC                 GT^AC
SapI          459  GAAGAGC              GCTCTTC(1/4)
SfaNI         552  GATGC                GCATC(5/9)
TseI          410  GCAGC                G^C[AT]GC
Tsp45I        591  GTCAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   14 enzymes) #
###################################

AcsI          194  AAATTC               [GA]^AATT[CT]
              496  AAATTT              
ApoI          194  AAATTC               [GA]^AATT[CT]
              496  AAATTT              
BstAPI         25  GCAAATCTTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
              397  GCAAACACTGC         
Cac8I          60  GCCTGC               GC[ACGT]^[ACGT]GC
              406  GCCAGC              
DpnII         444  GATC                 ^GATC
              646  GATC                
HaeIII        123  GGCC                 GG^CC
              468  GGCC                
HphI          378  GGTGA                GGTGA(8/7)
              563  TCACC               
MaeIII        187  GTAAC                ^GT[ACGT]AC
              591  GTCAC               
MboI          444  GATC                 ^GATC
              646  GATC                
MseI          287  TTAA                 T^TAA
              664  TTAA                
NdeII         444  GATC                 ^GATC
              646  GATC                
Sau3AI        444  GATC                 ^GATC
              646  GATC                
Tru9I         287  TTAA                 T^TAA
              664  TTAA                
XcmI          136  CCACTGCATACATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
              509  CCAAGTGCTTTATGG     


#########################################
# 3 OR MORE CUT ENZYMES (   16 enzymes) #
#########################################

AluI        BsaJI       BsmAI       BssKI       BstF5I      
BstNI       EcoRII      FokI        MboII       MnlI        
MvaI        MwoI        NlaIV       ScrFI       Tsp509I     
TspRI