Restriction map for
pFN21AE1721
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# NO CUT ENZYMES ( 152 enzymes) #
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Acc65I AccI AccII AclI AfaI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaI BsgI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrFI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstUI BstZ17I Bsu36I
Cac8I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI FbaI FseI HaeII HgaI
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MluI MluNI MscI MseI MslI
MspA1I MunI MvnI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuII RcaI
RsaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI Tru9I TspRI Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 53 enzymes) #
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AatII 543 GACGTC GACGT^C
AccIII 231 TCCGGA T^CCGGA
AcsI 456 GAATTC [GA]^AATT[CT]
AcyI 543 GACGTC G[GA]^CG[CT]C
AlwI 561 GGATC GGATC(4/5)
ApoI 456 GAATTC [GA]^AATT[CT]
AvaI 525 CTCGGG C^[CT]CG[GA]G
AvaII 144 GGTCC G^G[AT]CC
AviII 83 TGCGCA TGC^GCA
BanI 379 GGCACC G^G[CT][GA]CC
BcnI 36 CCGGG CC^[GC]GG
BsaHI 543 GACGTC G[GA]^CG[CT]C
BsaWI 231 TCCGGA [AT]^CCGG[AT]
BseAI 231 TCCGGA T^CCGGA
BseRI 30 GAGGAG GAGGAG(10/8)
BsiEI 71 CGATCG CG[GA][CT]^CG
BsmFI 145 GTCCC GGGAC(10/14)
BsoBI 525 CTCGGG C^[CT]CG[GA]G
BspEI 231 TCCGGA T^CCGGA
BspMI 8 GCAGGT ACCTGC(4/8)
BsrBI 344 CCGCTC CCGCTC(-3/-3)
BsrI 597 ACTGG ACTGG(1/-1)
BstXI 405 CCAGGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 561 GGATCT [GA]^GATC[CT]
CfoI 84 GCGC GCG^C
DraII 143 GGGTCCC [GA]G^G[ACGT]CC[CT]
EaeI 533 TGGCCG [CT]^GGCC[GA]
EarI 622 GAAGAG CTCTTC(1/4)
EcoO109I 143 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 456 GAATTC G^AATTC
EcoRV 49 GATATC GAT^ATC
EcoT22I 265 ATGCAT ATGCA^T
FauI 343 CCCGC CCCGC(4/6)
FspI 83 TGCGCA TGC^GCA
HhaI 84 GCGC GCG^C
Hin1I 543 GACGTC G[GA]^CG[CT]C
HinP1I 84 GCGC G^CGC
Ksp632I 622 GAAGAG CTCTTC(1/4)
MaeII 544 ACGT A^CGT
MflI 561 GGATCT [GA]^GATC[CT]
MroI 231 TCCGGA T^CCGGA
NciI 36 CCGGG CC^[GC]GG
NsiI 265 ATGCAT ATGCA^T
NspI 263 ACATGC [GA]CATG^[CT]
PleI 523 GACTC GAGTC(4/5)
PpuMI 143 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 523 GACTCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 71 CGATCG CGAT^CG
SacI 288 GAGCTC GAGCT^C
StuI 602 AGGCCT AGG^CCT
TfiI 131 GAATC G^A[AT]TC
Tsp509I 457 AATT ^AATT
XhoII 561 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
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AlwNI 428 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
559 CAGGATCTG
AspHI 288 GAGCTC G[AT]GC[AT]^C
421 GAGCAC
BbvI 329 GCAGC GCAGC(8/12)
510 GCAGC
BsiHKAI 288 GAGCTC G[AT]GC[AT]^C
421 GAGCAC
BsmBI 25 GAGACG CGTCTC(1/5)
541 GAGACG
BstEII 105 GGTGACC G^GT[ACGT]ACC
222 GGTGACC
BstPI 105 GGTGACC G^GT[ACGT]ACC
222 GGTGACC
EcoO65I 105 GGTGACC G^GT[ACGT]ACC
222 GGTGACC
EcoT14I 161 CCAAGG C^C[AT][AT]GG
389 CCAAGG
HapII 36 CCGG C^CGG
232 CCGG
HinfI 131 GAATC G^A[ACGT]TC
523 GACTC
HpaII 36 CCGG C^CGG
232 CCGG
MspI 36 CCGG C^CGG
232 CCGG
SfaNI 301 GATGC GCATC(5/9)
439 GATGC
StyI 161 CCAAGG C^C[AT][AT]GG
389 CCAAGG
TseI 329 GCAGC G^C[AT]GC
510 GCAGC
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AciI AluI BanII BmyI BpmI
BsaJI BsiYI BslI BsmAI Bsp1286I
BssKI BstF5I BstNI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HphI ItaI MaeIII MboI MboII
MnlI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI TaqI
Tsp45I TthHB8I