Restriction map for
pFN21AE1232
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BanI BbeI BbrPI BbvCI BcgI
BciVI BcnI BglI BglII BlnI
BpmI BsaAI BsaBI BsaHI BsaWI
BseAI BseRI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspHI BspMI
BsrBI BsrI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI CpoI DraII DraIII DrdI
Eam1105I EarI Eco47III Eco81I EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FseI
FspI Hin1I HincII HindII HpaI
KasI KpnI Ksp632I KspI MaeII
MamI MfeI MluNI MroI MscI
MunI NarI NciI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SapI SbfI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspI SwaI
TfiI Tth111I Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AcsI 328 AAATTC [GA]^AATT[CT]
AflII 694 CTTAAG C^TTAAG
AlwNI 439 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 328 AAATTC [GA]^AATT[CT]
AspHI 75 GAGCTC G[AT]GC[AT]^C
BamHI 3 GGATCC G^GATCC
BanII 75 GAGCTC G[GA]GC[CT]^C
BclI 321 TGATCA T^GATCA
BfaI 884 CTAG C^TAG
BfrI 694 CTTAAG C^TTAAG
BlpI 77 GCTCAGC GC^T[ACGT]AGC
BmyI 75 GAGCTC G[AGT]GC[ACT]^C
Bpu1102I 77 GCTCAGC GC^T[ACGT]AGC
BsaI 201 GAGACC GGTCTC(1/5)
BsgI 305 GTGCAG GTGCAG(16/14)
BsiEI 11 CGGCCG CG[GA][CT]^CG
BsiHKAI 75 GAGCTC G[AT]GC[AT]^C
BsmFI 248 GGGAC GGGAC(10/14)
Bsp1286I 75 GAGCTC G[AGT]GC[ACT]^C
Bsp1407I 897 TGTACA T^GTACA
BspLU11I 621 ACATGT A^CATGT
BsrGI 897 TGTACA T^GTACA
BstXI 1043 CCAACTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 3 GGATCC [GA]^GATC[CT]
CelII 77 GCTCAGC GC^T[ACGT]AGC
Cfr13I 552 GGCCC G^G[ACGT]CC
DraI 676 TTTAAA TTT^AAA
DsaI 14 CCGTGG C^C[GA][CT]GG
EaeI 11 CGGCCG [CT]^GGCC[GA]
EagI 11 CGGCCG C^GGCCG
EclXI 11 CGGCCG C^GGCCG
Eco52I 11 CGGCCG C^GGCCG
EcoNI 239 CCTTCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 321 TGATCA T^GATCA
HaeII 142 AGCGCC [GA]GCGC^[CT]
HindIII 166 AAGCTT A^AGCTT
MaeI 884 CTAG C^TAG
MflI 3 GGATCC [GA]^GATC[CT]
MluI 53 ACGCGT A^CGCGT
MspA1I 442 CAGCTG C[AC]G^C[GT]G
NlaIV 3 GGATCC GG[ACGT]^[ACGT]CC
PstI 138 CTGCAG CTGCA^G
PvuII 442 CAGCTG CAG^CTG
SacI 75 GAGCTC GAGCT^C
Sau96I 552 GGCCC G^G[ACGT]CC
ScaI 1080 AGTACT AGT^ACT
SfcI 138 CTGCAG C^T[GA][CT]AG
SmlI 694 CTTAAG C^T[CT][GA]AG
SphI 888 GCATGC GCATG^C
SspBI 897 TGTACA T^GTACA
StuI 236 AGGCCT AGG^CCT
TaqI 1069 TCGA T^CGA
TthHB8I 1069 TCGA T^CGA
XcmI 495 CCAGGAAGGATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 3 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AflIII 53 ACGCGT A^C[GA][CT]GT
621 ACATGT
BsmAI 201 GAGAC GTCTC(1/5)
878 GTCTC
BsrDI 60 CATTGC GCAATG(2/0)
865 GCAATG
BsrFI 9 GCCGGC [GA]^CCGG[CT]
67 GCCGGC
BssSI 556 CACGAG CACGAG(-5/-1)
944 CTCGTG
Cfr10I 9 GCCGGC [GA]^CCGG[CT]
67 GCCGGC
EcoT14I 548 CCAAGG C^C[AT][AT]GG
1089 CCTTGG
FauI 90 CCCGC CCCGC(4/6)
100 CCCGC
HgaI 52 GACGC GACGC(5/10)
172 GACGC
HphI 523 TCACC GGTGA(8/7)
1039 TCACC
MaeIII 215 GTGAC ^GT[ACGT]AC
1038 GTCAC
MslI 466 CATACCAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
970 CATCTCAGTG
NaeI 9 GCCGGC GCC^GGC
67 GCCGGC
NgoMIV 9 GCCGGC G^CCGGC
67 GCCGGC
SfaNI 1062 GATGC GCATC(5/9)
520 GCATC
StyI 548 CCAAGG C^C[AT][AT]GG
1089 CCTTGG
Tsp45I 215 GTGAC ^GT[GC]AC
1038 GTCAC
Tsp509I 148 AATT ^AATT
329 AATT
TspRI 457 CAGTG CA[GC]TG[ACGT][ACGT]^
975 CAGTG
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# 3 OR MORE CUT ENZYMES ( 46 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
BbsI BbvI BpuAI BsaJI BsiYI
BslI BssKI BstF5I BstNI BstUI
Cac8I CfoI DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII ItaI MboI
MboII MnlI MseI MspI MvaI
MvnI MwoI NdeII NlaIII NspI
PleI RsaI Sau3AI ScrFI Tru9I
TseI