##################################
# NO CUT ENZYMES (  176 enzymes) #
##################################

AatII       Acc65I      AccI        AclI        AcsI        
AcyI        AfaI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaLI       ApoI        AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspI        
AvaI        AviII       AvrII       BalI        BamHI       
BanI        BbeI        BbrPI       BbsI        BbvCI       
BcgI        BclI        BfrI        BglII       BlnI        
BlpI        Bpu1102I    BpuAI       BsaAI       BsaHI       
BsgI        BsiEI       BsiWI       BsmBI       BsmI        
BsoBI       Bsp1407I    BspDI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstYI       BstZ17I     Bsu36I      
CelII       Cfr10I      ClaI        CpoI        DpnII       
DraI        DraIII      DrdI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRI       EcoRV       EcoT14I     
EcoT22I     FbaI        FseI        FspI        HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
HphI        KasI        KpnI        KspI        MaeIII      
MboI        MfeI        MflI        MluI        MluNI       
MscI        MseI        MunI        NaeI        NarI        
NcoI        NdeI        NdeII       NgoMIV      NheI        
NlaIII      NotI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PvuI        RcaI        RsaI        
RsrII       SacI        SacII       SalI        Sau3AI      
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TfiI        Tru9I       
Tsp45I      Tsp509I     Tth111I     Van91I      XbaI        
XcmI        XhoI        XhoII       XmaCI       XmaI        
XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AccII          85  CGCG                 CG^CG
AccIII         52  TCCGGA               T^CCGGA
ApaI           59  GGGCCC               GGGCC^C
AspHI         149  GTGCTC               G[AT]GC[AT]^C
AvaII         104  GGACC                G^G[AT]CC
BciVI         286  GGATAC               GTATCC(6/5)
BfaI           46  CTAG                 C^TAG
BglI          172  GCCTTCCAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI         214  GATGAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI           18  GGTCTC               GGTCTC(1/5)
BsaWI          52  TCCGGA               [AT]^CCGG[AT]
BseAI          52  TCCGGA               T^CCGGA
BseRI          88  GAGGAG               GAGGAG(10/8)
BsiHKAI       149  GTGCTC               G[AT]GC[AT]^C
BsmAI          19  GTCTC                GTCTC(1/5)
BsmFI         103  GGGAC                GGGAC(10/14)
BspEI          52  TCCGGA               T^CCGGA
BsrI          292  ACTGG                ACTGG(1/-1)
BstUI          85  CGCG                 CG^CG
BstXI         263  CCACGCAGCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DdeI          194  CTGAG                C^T[ACGT]AG
DraII         251  GGGGCCT              [GA]G^G[ACGT]CC[CT]
DsaI           14  CCACGG               C^C[GA][CT]GG
EarI           67  CTCTTC               CTCTTC(1/4)
EcoO109I      251  GGGGCCT              [GA]G^G[ACGT]CC[CT]
FauI          249  GCGGG                CCCGC(4/6)
HinfI         279  GAGTC                G^A[ACGT]TC
Ksp632I        67  CTCTTC               CTCTTC(1/4)
MaeI           46  CTAG                 C^TAG
MaeII          74  ACGT                 A^CGT
MamI          214  GATGAGCATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI           52  TCCGGA               T^CCGGA
MslI          126  CATCCCGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI           85  CGCG                 CG^CG
NruI           84  TCGCGA               TCG^CGA
PleI          279  GAGTC                GAGTC(4/5)
PstI          154  CTGCAG               CTGCA^G
SapI           66  GCTCTTC              GCTCTTC(1/4)
SfaNI         219  GCATC                GCATC(5/9)
TaqI           71  TCGA                 T^CGA
TspRI         291  CACTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I        71  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AciI           94  GCGG                 CCGC(-3/-1)
              249  GCGG                
BanII          59  GGGCCC               G[GA]GC[CT]^C
              296  GGGCTC              
BsiYI          50  CCTCCGGACGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              107  CCATCCCAGGG         
BslI           50  CCTCCGGACGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              107  CCATCCCAGGG         
BstF5I        108  CATCC                GGATG(2/0)
              126  CATCC               
FokI          108  CATCC                GGATG(9/13)
              126  CATCC               
HaeII           9  AGCGCC               [GA]GCGC^[CT]
              117  GGCGCT              
SfcI          154  CTGCAG               C^T[GA][CT]AG
              318  CTATAG              


#########################################
# 3 OR MORE CUT ENZYMES (   32 enzymes) #
#########################################

AluI        BbvI        BcnI        BmyI        BpmI        
BsaJI       Bsp1286I    BssKI       BstNI       Cac8I       
CfoI        Cfr13I      EcoRII      Fnu4HI      HaeIII      
HapII       HhaI        HinP1I      HpaII       ItaI        
MboII       MnlI        MspA1I      MspI        MvaI        
MwoI        NciI        NlaIV       PvuII       Sau96I      
ScrFI       TseI