##################################
# NO CUT ENZYMES (  207 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaI        
AvaII       AviII       AvrII       BalI        BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BciVI       BclI        BcnI        
BfaI        BfrI        BglII       BlnI        BlpI        
Bpu1102I    BpuAI       BsaAI       BsaBI       BsaHI       
BsaI        BsaWI       BseAI       BseRI       BsgI        
BsiEI       BsiHKAI     BsiWI       BsiYI       BslI        
BsmAI       BsmBI       BsmFI       BsmI        BsoBI       
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrFI       BsrGI       BsrI        
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      CelII       Cfr10I      ClaI        
CpoI        DpnII       DraI        DraII       DraIII      
DrdI        EagI        Eam1105I    EarI        EclXI       
Eco47III    Eco52I      Eco81I      EcoNI       EcoO109I    
EcoO65I     EcoRI       EcoRV       EcoT22I     FbaI        
FseI        FspI        HaeII       HapII       HgaI        
Hin1I       HincII      HindII      HindIII     HpaI        
HpaII       HphI        KasI        KpnI        Ksp632I     
KspI        MaeI        MaeII       MamI        MboI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MslI        MspI        MunI        MvnI        
NaeI        NarI        NciI        NdeI        NdeII       
NgoMIV      NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflFI       PflMI       
PinAI       PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
RcaI        RsaI        RsrII       SacI        SacII       
SalI        SapI        Sau3AI      SbfI        ScaI        
SexAI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SmlI        SnaBI       SpeI        
SphI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        Tsp509I     Tth111I     TthHB8I     
Van91I      XbaI        XhoI        XhoII       XmaCI       
XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   31 enzymes) #
###################################

BglI           19  GCCATCGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BmyI          335  GGGCAC               G[AGT]GC[ACT]^C
BpmI           81  CTCCAG               CTGGAG(16/14)
Bsp1286I      335  GGGCAC               G[AGT]GC[ACT]^C
BsrDI          75  CATTGC               GCAATG(2/0)
BssKI         126  CCTGG                ^CC[ACGT]GG
BstNI         126  CCTGG                CC^[AT]GG
CfoI          204  GCGC                 GCG^C
Cfr13I         45  GGCCC                G^G[ACGT]CC
DdeI           57  CTCAG                C^T[ACGT]AG
DsaI          312  CCATGG               C^C[GA][CT]GG
EaeI          363  CGGCCA               [CT]^GGCC[GA]
EcoRII        126  CCTGG                ^CC[AT]GG
FauI          225  CCCGC                CCCGC(4/6)
HhaI          204  GCGC                 GCG^C
HinP1I        204  GCGC                 G^CGC
MseI          242  TTAA                 T^TAA
MspA1I         84  CAGCTG               C[AC]G^C[GT]G
MvaI          126  CCTGG                CC^[AT]GG
NcoI          312  CCATGG               C^CATGG
NlaIV         122  GGCTCC               GG[ACGT]^[ACGT]CC
NspI           51  ACATGC               [GA]CATG^[CT]
PleI           14  GACTC                GAGTC(4/5)
PvuII          84  CAGCTG               CAG^CTG
Sau96I         45  GGCCC                G^G[ACGT]CC
ScrFI         126  CCTGG                CC^[ACGT]GG
SfaNI         149  GCATC                GCATC(5/9)
TfiI          212  GAATC                G^A[AT]TC
Tru9I         242  TTAA                 T^TAA
Tsp45I        173  GTGAC                ^GT[GC]AC
TspRI         247  CAGTG                CA[GC]TG[ACGT][ACGT]^


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AciI          226  CCGC                 CCGC(-3/-1)
               43  GCGG                
BsaJI          29  CCTTGG               C^C[ACGT][ACGT]GG
              312  CCATGG              
Cac8I         224  GCCCGC               GC[ACGT]^[ACGT]GC
              261  GCAAGC              
EcoT14I        29  CCTTGG               C^C[AT][AT]GG
              312  CCATGG              
HinfI          14  GACTC                G^A[ACGT]TC
              212  GAATC               
MaeIII        173  GTGAC                ^GT[ACGT]AC
              329  GTAAC               
MboII         209  GAAGA                GAAGA(8/7)
              303  GAAGA               
StyI           29  CCTTGG               C^C[AT][AT]GG
              312  CCATGG              


#########################################
# 3 OR MORE CUT ENZYMES (   12 enzymes) #
#########################################

AluI        BbvI        BstF5I      Fnu4HI      FokI        
HaeIII      ItaI        MnlI        MwoI        NlaIII      
TseI        XcmI