Restriction map for
pFN21AE1263
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII AccI AccIII AclI AflII
AflIII AgeI AocI Aor51HI ApaI
AscI AseI AsnI AspI AvaI
AvrII BbrPI BcgI BfrI BlnI
BlpI Bpu1102I BseAI BsiWI BsmI
BsoBI Bsp1407I BspEI BspHI BspLU11I
BsrBI BsrGI BssHII Bst1107I BstBI
BstZ17I Bsu36I CelII CpoI DraI
DraII DraIII DsaI EagI EclXI
Eco47III Eco52I Eco81I EcoO109I EcoRI
EcoRV EcoT22I FseI HpaI KspI
MfeI MluI MroI MslI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfiI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI SwaI Tth111I XbaI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
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Acc65I 927 GGTACC G^GTACC
AcyI 65 GGCGCC G[GA]^CG[CT]C
AhdI 854 GACGCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1580 GTGCAC G^TGCAC
ApaLI 1580 GTGCAC G^TGCAC
Asp700I 1705 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 927 GGTACC G^GTACC
AspEI 854 GACGCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 443 TGCGCA TGC^GCA
BalI 1010 TGGCCA TGG^CCA
BamHI 1345 GGATCC G^GATCC
BbeI 65 GGCGCC GGCGC^C
BbvCI 1660 GCTGAGG CCTCAGC(-5/-2)
BciVI 894 GGATAC GTATCC(6/5)
BclI 744 TGATCA T^GATCA
BfaI 436 CTAG C^TAG
BglI 1628 GCCCTGATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 138 AGATCT A^GATCT
BsaAI 1642 TACGTG [CT]AC^GT[GA]
BsaHI 65 GGCGCC G[GA]^CG[CT]C
BsaI 757 GAGACC GGTCTC(1/5)
BsaWI 1286 ACCGGA [AT]^CCGG[AT]
BseRI 97 GAGGAG GAGGAG(10/8)
BsiEI 1284 CGACCG CG[GA][CT]^CG
BspDI 1193 ATCGAT AT^CGAT
BspMI 440 ACCTGC ACCTGC(4/8)
BsrFI 74 ACCGGC [GA]^CCGG[CT]
BssSI 199 CACGAG CACGAG(-5/-1)
BstEII 570 GGTCACC G^GT[ACGT]ACC
BstPI 570 GGTCACC G^GT[ACGT]ACC
BstXI 575 CCAGATGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 74 ACCGGC [GA]^CCGG[CT]
ClaI 1193 ATCGAT AT^CGAT
DrdI 715 GACATTGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1010 TGGCCA [CT]^GGCC[GA]
Eam1105I 854 GACGCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 811 CCTGCTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 570 GGTCACC G^GT[ACGT]ACC
EcoT14I 524 CCTTGG C^C[AT][AT]GG
FauI 32 CCCGC CCCGC(4/6)
FbaI 744 TGATCA T^GATCA
FspI 443 TGCGCA TGC^GCA
HgaI 854 GACGC GACGC(5/10)
Hin1I 65 GGCGCC G[GA]^CG[CT]C
HincII 973 GTCAAC GT[CT]^[GA]AC
HindII 973 GTCAAC GT[CT]^[GA]AC
KasI 65 GGCGCC G^GCGCC
KpnI 927 GGTACC GGTAC^C
MaeI 436 CTAG C^TAG
MluNI 1010 TGGCCA TGG^CCA
MscI 1010 TGGCCA TGG^CCA
NarI 65 GGCGCC GG^CGCC
PflMI 561 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 1504 GAGCTC GAGCT^C
SfoI 65 GGCGCC GGC^GCC
StuI 819 AGGCCT AGG^CCT
StyI 524 CCTTGG C^C[AT][AT]GG
Van91I 561 CCAGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 1705 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AccII 16 CGCG CG^CG
1262 CGCG
AcsI 1117 GAATTT [GA]^AATT[CT]
1409 AAATTC
ApoI 1117 GAATTT [GA]^AATT[CT]
1409 AAATTC
BanI 65 GGCGCC G^G[CT][GA]CC
927 GGTACC
BanII 1504 GAGCTC G[GA]GC[CT]^C
1665 GGGCTC
BbsI 949 GTCTTC GAAGAC(2/6)
1621 GTCTTC
BpuAI 949 GTCTTC GAAGAC(2/6)
1621 GTCTTC
BsaBI 895 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1186 GATGATTATC
BsgI 1368 CTGCAC GTGCAG(16/14)
982 GTGCAG
BsmBI 424 GAGACG CGTCTC(1/5)
852 GAGACG
BsrDI 1355 GCAATG GCAATG(2/0)
1377 GCAATG
BstUI 16 CGCG CG^CG
1262 CGCG
HaeII 41 AGCGCC [GA]GCGC^[CT]
65 GGCGCC
HindIII 451 AAGCTT A^AGCTT
1039 AAGCTT
HphI 327 GGTGA GGTGA(8/7)
572 TCACC
MamI 895 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1186 GATGATTATC
MvnI 16 CGCG CG^CG
1262 CGCG
TfiI 829 GATTC G^A[AT]TC
1172 GAATC
XcmI 1013 CCACTGGAGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1736 CCATCAATGCGGTGG
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# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BbvI BcnI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EarI
EcoRII Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
Ksp632I MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII