Restriction map for
pFN21AE2060
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# NO CUT ENZYMES ( 109 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BbeI BbvCI BcgI BfrI BlnI
BlpI Bpu1102I BsaBI BsaI BsaWI
BseAI BsiWI BsmAI BsmBI BspDI
BspEI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MroI MseI MunI NarI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspI SwaI Tru9I
Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccII 1456 CGCG CG^CG
AcsI 310 GAATTT [GA]^AATT[CT]
AcyI 1942 GACGCC G[GA]^CG[CT]C
AflIII 1907 ACATGT A^C[GA][CT]GT
AocI 1818 CCTCAGG CC^T[ACGT]AGG
ApaI 1466 GGGCCC GGGCC^C
ApoI 310 GAATTT [GA]^AATT[CT]
AvaI 297 CCCGGG C^[CT]CG[GA]G
BalI 2006 TGGCCA TGG^CCA
BanI 21 GGTGCC G^G[CT][GA]CC
BbrPI 1086 CACGTG CAC^GTG
BclI 140 TGATCA T^GATCA
BglI 527 GCCGTCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1942 GACGCC G[GA]^CG[CT]C
BsiEI 1479 CGACCG CG[GA][CT]^CG
BsoBI 297 CCCGGG C^[CT]CG[GA]G
BspLU11I 1907 ACATGT A^CATGT
BsrBI 679 GAGCGG CCGCTC(-3/-3)
BsrFI 24 GCCGGC [GA]^CCGG[CT]
BstAPI 2030 GCATCGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 1456 CGCG CG^CG
Bsu36I 1818 CCTCAGG CC^T[ACGT]AGG
Cfr10I 24 GCCGGC [GA]^CCGG[CT]
EaeI 2006 TGGCCA [CT]^GGCC[GA]
EarI 1579 GAAGAG CTCTTC(1/4)
Eco81I 1818 CCTCAGG CC^T[ACGT]AGG
EcoNI 199 CCTTCTGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 140 TGATCA T^GATCA
Hin1I 1942 GACGCC G[GA]^CG[CT]C
Ksp632I 1579 GAAGAG CTCTTC(1/4)
MluNI 2006 TGGCCA TGG^CCA
MscI 2006 TGGCCA TGG^CCA
MvnI 1456 CGCG CG^CG
NaeI 24 GCCGGC GCC^GGC
NgoMIV 24 GCCGGC G^CCGGC
NspI 1907 ACATGT [GA]CATG^[CT]
PmaCI 1086 CACGTG CAC^GTG
PmlI 1086 CACGTG CAC^GTG
PshAI 1633 GACTACCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 1582 GAGCTC GAGCT^C
SapI 1579 GAAGAGC GCTCTTC(1/4)
ScaI 1208 AGTACT AGT^ACT
SexAI 1043 ACCAGGT A^CC[AT]GGT
SmaI 297 CCCGGG CCC^GGG
SmlI 700 CTCAAG C^T[CT][GA]AG
StuI 1853 AGGCCT AGG^CCT
TaqI 827 TCGA T^CGA
TfiI 1329 GATTC G^A[AT]TC
TthHB8I 827 TCGA T^CGA
XcmI 1950 CCAGGGGGATAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 297 CCCGGG C^CCGGG
XmaI 297 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AspHI 1582 GAGCTC G[AT]GC[AT]^C
2068 GTGCTC
BamHI 465 GGATCC G^GATCC
1487 GGATCC
BanII 1466 GGGCCC G[GA]GC[CT]^C
1582 GAGCTC
BbsI 550 GAAGAC GAAGAC(2/6)
175 GTCTTC
BfaI 943 CTAG C^TAG
1594 CTAG
BglII 230 AGATCT A^GATCT
767 AGATCT
BpuAI 550 GAAGAC GAAGAC(2/6)
175 GTCTTC
BsaAI 1086 CACGTG [CT]AC^GT[GA]
2077 TACGTG
BsiHKAI 1582 GAGCTC G[AT]GC[AT]^C
2068 GTGCTC
BsmI 558 GCATTC GAATGC(1/-1)
1053 GCATTC
Bsp1407I 590 TGTACA T^GTACA
1277 TGTACA
BspHI 1586 TCATGA T^CATGA
1812 TCATGA
BsrDI 1002 CATTGC GCAATG(2/0)
839 GCAATG
BsrGI 590 TGTACA T^GTACA
1277 TGTACA
BstXI 1074 CCAGGATGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1169 CCACCACAATGG
EcoT14I 321 CCAAGG C^C[AT][AT]GG
1517 CCAAGG
HgaI 1942 GACGC GACGC(5/10)
1622 GCGTC
MaeI 943 CTAG C^TAG
1594 CTAG
PflMI 414 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1345 CCAGTGTGTGG
PpuMI 1707 GGGTCCC [GA]G^G[AT]CC[CT]
1982 GGGACCC
PstI 36 CTGCAG CTGCA^G
970 CTGCAG
PvuII 1064 CAGCTG CAG^CTG
1496 CAGCTG
RcaI 1586 TCATGA T^CATGA
1812 TCATGA
SspBI 590 TGTACA T^GTACA
1277 TGTACA
StyI 321 CCAAGG C^C[AT][AT]GG
1517 CCAAGG
Tsp509I 311 AATT ^AATT
1657 AATT
Van91I 414 CCAAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1345 CCAGTGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 70 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BbvI BciVI BcnI BmyI
BpmI BsaJI BseRI BsgI BsiYI
BslI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MaeII
MaeIII MboI MboII MflI MnlI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI TseI Tsp45I TspRI XhoII