##################################
# NO CUT ENZYMES (  197 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AfaI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AspHI       AspI        AvaII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BfrI        BglI        BglII       BlnI        
BlpI        BmyI        BpmI        Bpu1102I    BsaAI       
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BseRI       BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmFI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BsrDI       BsrFI       
BsrGI       BssHII      BssSI       Bst1107I    BstAPI      
BstBI       BstEII      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      CelII       Cfr10I      
Cfr13I      ClaI        CpoI        DdeI        DpnII       
DraI        DraII       DraIII      DrdI        EaeI        
EagI        Eam1105I    EclXI       Eco47III    Eco52I      
Eco81I      EcoO109I    EcoO65I     EcoRI       EcoRV       
EcoT22I     FbaI        FseI        HaeII       HaeIII      
HgaI        Hin1I       HincII      HindII      HindIII     
HinfI       HpaI        KasI        KpnI        KspI        
MaeII       MamI        MboI        MfeI        MflI        
MluI        MluNI       MroI        MscI        MseI        
MslI        MspA1I      MunI        MvnI        NaeI        
NarI        NdeI        NdeII       NgoMIV      NheI        
NlaIV       NotI        NruI        NsiI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PleI        PmaCI       PmeI        PmlI        PpuMI       
PshAI       PshBI       Psp1406I    PstI        PvuI        
PvuII       RcaI        RsaI        RsrII       SacI        
SacII       SalI        SapI        Sau3AI      Sau96I      
SbfI        ScaI        SexAI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmlI        SnaBI       
SpeI        Sse8387I    SspBI       SspI        StuI        
SwaI        TaqI        TfiI        Tru9I       Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AvaI          191  CCCGGG               C^[CT]CG[GA]G
AviII         222  TGCGCA               TGC^GCA
BbsI            3  GTCTTC               GAAGAC(2/6)
BfaI           25  CTAG                 C^TAG
BpuAI           3  GTCTTC               GAAGAC(2/6)
BsgI           79  GTGCAG               GTGCAG(16/14)
BsmI           68  GAATGC               GAATGC(1/-1)
BsoBI         191  CCCGGG               C^[CT]CG[GA]G
BspMI         225  GCAGGT               ACCTGC(4/8)
BsrI          149  CCAGT                ACTGG(1/-1)
BstNI         132  CCAGG                CC^[AT]GG
CfoI          223  GCGC                 GCG^C
DsaI          254  CCATGG               C^C[GA][CT]GG
EarI          112  GAAGAG               CTCTTC(1/4)
EcoNI          10  CCTCCCGAAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRII        132  CCAGG                ^CC[AT]GG
EcoT14I       254  CCATGG               C^C[AT][AT]GG
FspI          222  TGCGCA               TGC^GCA
HhaI          223  GCGC                 GCG^C
HinP1I        223  GCGC                 G^CGC
Ksp632I       112  GAAGAG               CTCTTC(1/4)
MaeI           25  CTAG                 C^TAG
MaeIII        197  GTGAC                ^GT[ACGT]AC
MvaI          132  CCAGG                CC^[AT]GG
NcoI          254  CCATGG               C^CATGG
NspI          246  GCATGC               [GA]CATG^[CT]
SfaNI         264  GCATC                GCATC(5/9)
SmaI          191  CCCGGG               CCC^GGG
SphI          246  GCATGC               GCATG^C
StyI          254  CCATGG               C^C[AT][AT]GG
Tsp45I        197  GTGAC                ^GT[GC]AC
Tsp509I        75  AATT                 ^AATT
TspRI         165  CACTG                CA[GC]TG[ACGT][ACGT]^
XmaCI         191  CCCGGG               C^CCGGG
XmaI          191  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AluI           36  AGCT                 AG^CT
               60  AGCT                
BbvI          294  GCAGC                GCAGC(8/12)
              220  GCTGC               
BsmAI          28  GAGAC                GTCTC(1/5)
              101  GAGAC               
Cac8I          48  GCCCGC               GC[ACGT]^[ACGT]GC
              246  GCATGC              
FauI           45  CCCGC                CCCGC(4/6)
               49  CCCGC               
HapII         192  CCGG                 C^CGG
              214  CCGG                
HpaII         192  CCGG                 C^CGG
              214  CCGG                
HphI          196  GGTGA                GGTGA(8/7)
              234  TCACC               
MboII         112  GAAGA                GAAGA(8/7)
                4  TCTTC               
MnlI           10  CCTC                 CCTC(7/6)
              251  CCTC                
MspI          192  CCGG                 C^CGG
              214  CCGG                
NlaIII        247  CATG                 CATG^
              255  CATG                
TseI          220  GCTGC                G^C[AT]GC
              294  GCAGC               


#########################################
# 3 OR MORE CUT ENZYMES (   13 enzymes) #
#########################################

AciI        BcnI        BsaJI       BsiYI       BslI        
BssKI       BstF5I      Fnu4HI      FokI        ItaI        
MwoI        NciI        ScrFI