Restriction map for
pFN21AE1274
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# NO CUT ENZYMES ( 108 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp718I
AspEI AspHI AspI AviII AvrII
BbrPI BbvCI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaWI
BseAI BseRI BsiEI BsiHKAI BsiWI
BspDI BspEI BspHI BsrDI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I CelII
ClaI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRV FseI FspI HgaI HpaI
KpnI KspI MamI MfeI MluI
MroI MunI MvnI NaeI NdeI
NgoMIV NheI NotI NruI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RcaI
SacI SacII SalI SapI SbfI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI Sse8387I StuI SwaI
Tth111I XbaI XcmI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 636 GTCTAC GT^[AC][GT]AC
AcyI 664 GGCGCC G[GA]^CG[CT]C
AseI 2098 ATTAAT AT^TAAT
AsnI 2098 ATTAAT AT^TAAT
BamHI 1432 GGATCC G^GATCC
BanII 38 GAGCCC G[GA]GC[CT]^C
BbeI 664 GGCGCC GGCGC^C
BcgI 147 CGAGCGGAATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 2108 AGATCT A^GATCT
BmyI 38 GAGCCC G[AGT]GC[ACT]^C
BsaHI 664 GGCGCC G[GA]^CG[CT]C
BsaI 2049 GGTCTC GGTCTC(1/5)
BsmBI 1745 GAGACG CGTCTC(1/5)
Bsp1286I 38 GAGCCC G[AGT]GC[ACT]^C
Bsp1407I 137 TGTACA T^GTACA
BspMI 570 ACCTGC ACCTGC(4/8)
BsrFI 219 GCCGGT [GA]^CCGG[CT]
BsrGI 137 TGTACA T^GTACA
BstXI 938 CCACCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 219 GCCGGT [GA]^CCGG[CT]
CpoI 163 CGGACCG CG^G[AT]CCG
DraI 1240 TTTAAA TTT^AAA
EcoNI 447 CCTTGGGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1282 GAATTC G^AATTC
EcoT22I 962 ATGCAT ATGCA^T
Hin1I 664 GGCGCC G[GA]^CG[CT]C
HincII 976 GTCAAC GT[CT]^[GA]AC
HindII 976 GTCAAC GT[CT]^[GA]AC
KasI 664 GGCGCC G^GCGCC
NarI 664 GGCGCC GG^CGCC
NcoI 2036 CCATGG C^CATGG
NsiI 962 ATGCAT ATGCA^T
PaeR7I 1994 CTCGAG C^TCGAG
PleI 2124 GACTC GAGTC(4/5)
PshBI 2098 ATTAAT AT^TAAT
PstI 873 CTGCAG CTGCA^G
RsrII 163 CGGACCG CG^G[AT]CCG
ScaI 590 AGTACT AGT^ACT
SfcI 873 CTGCAG C^T[GA][CT]AG
SfoI 664 GGCGCC GGC^GCC
SmaI 41 CCCGGG CCC^GGG
SspBI 137 TGTACA T^GTACA
SspI 1885 AATATT AAT^ATT
XhoI 1994 CTCGAG C^TCGAG
XmaCI 41 CCCGGG C^CCGGG
XmaI 41 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 17 enzymes) #
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AlwNI 2088 CAGGTTCTG CAG[ACGT][ACGT][ACGT]^CTG
2122 CAGACTCTG
BanI 51 GGTGCC G^G[CT][GA]CC
664 GGCGCC
BciVI 672 GGATAC GTATCC(6/5)
1329 GGATAC
BclI 86 TGATCA T^GATCA
1973 TGATCA
BsmAI 1745 GAGAC GTCTC(1/5)
2050 GTCTC
BsmFI 14 GGGAC GGGAC(10/14)
1465 GGGAC
BspLU11I 194 ACATGT A^CATGT
1537 ACATGT
BsrBI 148 GAGCGG CCGCTC(-3/-3)
160 GAGCGG
DraII 1005 AGGTCCT [GA]G^G[ACGT]CC[CT]
1465 GGGACCC
DsaI 1795 CCACGG C^C[GA][CT]GG
2036 CCATGG
EcoO109I 1005 AGGTCCT [GA]G^G[ACGT]CC[CT]
1465 GGGACCC
FauI 95 CCCGC CCCGC(4/6)
2054 CCCGC
FbaI 86 TGATCA T^GATCA
1973 TGATCA
HindIII 1236 AAGCTT A^AGCTT
1720 AAGCTT
PpuMI 1005 AGGTCCT [GA]G^G[AT]CC[CT]
1465 GGGACCC
PvuII 605 CAGCTG CAG^CTG
1495 CAGCTG
SmlI 1914 CTTGAG C^T[CT][GA]AG
1994 CTCGAG
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# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI Asp700I AvaI AvaII
BalI BbsI BbvI BcnI BfaI
BpmI BpuAI BsaJI BsgI BsiYI
BslI BsmI BsoBI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EaeI EarI
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
RsaI Sau3AI Sau96I ScrFI SfaNI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XhoII XmnI