Restriction map for
pFN21AE1306
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# NO CUT ENZYMES ( 127 enzymes) #
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AccI AccII AccIII AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I AspEI AspHI AvrII
BamHI BbeI BcgI BclI BfaI
BfrI BglII BlnI BsaBI BsaWI
BseAI BsiEI BsiHKAI BsiWI BspDI
BspEI BspLU11I BsrDI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstYI BstZ17I ClaI CpoI
DraI DraIII EagI Eam1105I EarI
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI HaeII
HincII HindII HindIII HpaI KasI
Ksp632I KspI MaeI MamI MfeI
MflI MluI MroI MseI MslI
MunI MvnI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmeI
PshAI PshBI Psp1406I PvuI RsrII
SacI SacII SalI SapI ScaI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SspI SwaI TfiI Tru9I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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Acc65I 485 GGTACC G^GTACC
AflIII 641 ACGTGT A^C[GA][CT]GT
AlwNI 124 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 485 GGTACC G^GTACC
AvaI 1087 CCCGAG C^[CT]CG[GA]G
AviII 724 TGCGCA TGC^GCA
BanI 485 GGTACC G^G[CT][GA]CC
BbrPI 640 CACGTG CAC^GTG
BbsI 1045 GTCTTC GAAGAC(2/6)
BciVI 960 GTATCC GTATCC(6/5)
BglI 407 GCCTGATCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 976 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 976 GCTGAGC GC^T[ACGT]AGC
BpuAI 1045 GTCTTC GAAGAC(2/6)
BsaAI 640 CACGTG [CT]AC^GT[GA]
BsaI 340 GAGACC GGTCTC(1/5)
BsmBI 951 CGTCTC CGTCTC(1/5)
BsmI 371 GCATTC GAATGC(1/-1)
BsoBI 1087 CCCGAG C^[CT]CG[GA]G
Bsp1407I 248 TGTACA T^GTACA
BspHI 77 TCATGA T^CATGA
BspMI 1034 ACCTGC ACCTGC(4/8)
BsrBI 6 GAGCGG CCGCTC(-3/-3)
BsrGI 248 TGTACA T^GTACA
CelII 976 GCTGAGC GC^T[ACGT]AGC
DrdI 85 GACTCCCCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 893 CCGTGG C^C[GA][CT]GG
EcoNI 1074 CCTCACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 724 TGCGCA TGC^GCA
HinfI 85 GACTC G^A[ACGT]TC
KpnI 485 GGTACC GGTAC^C
PleI 85 GACTC GAGTC(4/5)
PmaCI 640 CACGTG CAC^GTG
PmlI 640 CACGTG CAC^GTG
RcaI 77 TCATGA T^CATGA
SbfI 1127 CCTGCAGG CCTGCA^GG
SphI 479 GCATGC GCATG^C
Sse8387I 1127 CCTGCAGG CCTGCA^GG
SspBI 248 TGTACA T^GTACA
StuI 380 AGGCCT AGG^CCT
Tsp509I 808 AATT ^AATT
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AatII 751 GACGTC GACGT^C
949 GACGTC
AlwI 74 GGATC GGATC(4/5)
861 GGATC
AocI 756 CCTGAGG CC^T[ACGT]AGG
934 CCTGAGG
AspI 307 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
748 GACGACGTC
BalI 401 TGGCCA TGG^CCA
650 TGGCCA
BbvCI 210 GCTGAGG CCTCAGC(-5/-2)
1038 GCTGAGG
BpmI 295 CTGGAG CTGGAG(16/14)
943 CTGGAG
BsgI 39 CTGCAC GTGCAG(16/14)
819 CTGCAC
Bsu36I 756 CCTGAGG CC^T[ACGT]AGG
934 CCTGAGG
Eco81I 756 CCTGAGG CC^T[ACGT]AGG
934 CCTGAGG
FauI 1104 CCCGC CCCGC(4/6)
459 GCGGG
MaeIII 355 GTCAC ^GT[ACGT]AC
576 GTCAC
MboII 695 TCTTC GAAGA(8/7)
1046 TCTTC
MluNI 401 TGGCCA TGG^CCA
650 TGGCCA
MscI 401 TGGCCA TGG^CCA
650 TGGCCA
NspI 320 GCATGT [GA]CATG^[CT]
479 GCATGC
PflFI 307 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
748 GACGACGTC
TaqI 629 TCGA T^CGA
1049 TCGA
Tsp45I 355 GTCAC ^GT[GC]AC
576 GTCAC
Tth111I 307 GACGTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
748 GACGACGTC
TthHB8I 629 TCGA T^CGA
1049 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcyI AfaI AluI AvaII
BanII BbvI BcnI BmyI BsaHI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrFI BsrI BssKI
BstF5I BstNI BstXI Cac8I CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII HphI ItaI
MaeII MboI MnlI MspA1I MspI
MvaI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfcI StyI TseI TspRI