##################################
# NO CUT ENZYMES (  220 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        AluI        Alw44I      AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BcgI        BciVI       BclI        BfaI        BfrI        
BglI        BglII       BlnI        BlpI        BmyI        
BpmI        Bpu1102I    BpuAI       BsaAI       BsaBI       
BsaHI       BsaI        BsaWI       BseAI       BseRI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsmBI       
BsmI        BsoBI       Bsp1286I    Bsp1407I    BspDI       
BspEI       BspHI       BspLU11I    BspMI       BsrBI       
BsrDI       BsrFI       BsrGI       BsrI        BssHII      
Bst1107I    BstAPI      BstBI       BstEII      BstF5I      
BstNI       BstPI       BstUI       BstXI       BstYI       
BstZ17I     Bsu36I      Cac8I       CelII       Cfr10I      
Cfr13I      ClaI        CpoI        DdeI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRII      EcoRV       EcoT14I     EcoT22I     
FauI        FbaI        FokI        FseI        FspI        
HaeII       HaeIII      HgaI        Hin1I       HincII      
HindII      HindIII     HpaI        HphI        KasI        
KpnI        Ksp632I     KspI        MaeI        MaeII       
MaeIII      MamI        MboII       MfeI        MflI        
MluI        MluNI       MnlI        MroI        MscI        
MseI        MslI        MunI        MvaI        MvnI        
MwoI        NaeI        NarI        NcoI        NdeI        
NgoMIV      NheI        NlaIII      NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PinAI       PmaCI       PmeI        PmlI        
PpuMI       PshAI       PshBI       Psp1406I    PstI        
PvuI        PvuII       RcaI        RsrII       SacI        
SacII       SalI        SapI        Sau96I      SbfI        
ScaI        SexAI       SfcI        SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TfiI        Tru9I       
Tsp45I      Tsp509I     TspRI       Tth111I     XbaI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AfaI           84  GTAC                 GT^AC
AlwI            3  GATCC                GGATC(4/5)
BbvI            8  GCTGC                GCAGC(8/12)
BcnI           99  CCGGG                CC^[GC]GG
BsaJI          99  CCGGGG               C^C[ACGT][ACGT]GG
BsmAI          56  GTCTC                GTCTC(1/5)
BsmFI          23  GTCCC                GGGAC(10/14)
BssKI          99  CCGGG                ^CC[ACGT]GG
BssSI          45  CTCGTG               CACGAG(-5/-1)
CfoI           75  GCGC                 GCG^C
DpnII           3  GATC                 ^GATC
Fnu4HI          8  GCTGC                GC^[ACGT]GC
HapII          99  CCGG                 C^CGG
HhaI           75  GCGC                 GCG^C
HinP1I         75  GCGC                 G^CGC
HinfI          54  GAGTC                G^A[ACGT]TC
HpaII          99  CCGG                 C^CGG
ItaI            8  GCTGC                GC^[ACGT]GC
MboI            3  GATC                 ^GATC
MspA1I          6  CCGCTG               C[AC]G^C[GT]G
MspI           99  CCGG                 C^CGG
NciI           99  CCGGG                CC^[GC]GG
NdeII           3  GATC                 ^GATC
NlaIV          35  GGTTCC               GG[ACGT]^[ACGT]CC
PflMI          26  CCATCGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI           54  GAGTC                GAGTC(4/5)
RsaI           84  GTAC                 GT^AC
Sau3AI          3  GATC                 ^GATC
ScrFI          99  CCGGG                CC^[ACGT]GG
SfaNI          95  GATGC                GCATC(5/9)
TaqI           29  TCGA                 T^CGA
TseI            8  GCTGC                G^C[AT]GC
TthHB8I        29  TCGA                 T^CGA
Van91I         26  CCATCGAGTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI           70  CCAAAGCGCAAGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (    3 enzymes) #
###################################

AciI            6  CCGC                 CCGC(-3/-1)
               39  CCGC                
BsiYI          26  CCATCGAGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               92  CCAGATGCCGG         
BslI           26  CCATCGAGTGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
               92  CCAGATGCCGG         


#########################################
# 3 OR MORE CUT ENZYMES (    0 enzymes) #
#########################################