Restriction map for
pFN21AE2115
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# NO CUT ENZYMES ( 145 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AvrII BamHI BanI
BbeI BbrPI BbsI BbvCI BcgI
BciVI BfaI BfrI BglI BglII
BlnI BlpI BpmI Bpu1102I BpuAI
BsaAI BsaBI BsaHI BsaI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspMI BsrBI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstUI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoRI EcoRV
EcoT22I FauI FseI HaeII Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeI MamI MfeI
MflI MluI MroI MspA1I MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflMI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
PvuII RcaI RsrII SacII SalI
SapI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
SspBI SspI StuI SwaI Tsp509I
Van91I XbaI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AfaI 471 GTAC GT^AC
AgeI 712 ACCGGT A^CCGGT
AlwI 640 GATCC GGATC(4/5)
AspI 435 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 633 CTCGAG C^[CT]CG[GA]G
AviII 521 TGCGCA TGC^GCA
BalI 58 TGGCCA TGG^CCA
BclI 362 TGATCA T^GATCA
BsaWI 712 ACCGGT [AT]^CCGG[AT]
BseRI 778 CTCCTC GAGGAG(10/8)
BsgI 178 CTGCAC GTGCAG(16/14)
BsmAI 127 GAGAC GTCTC(1/5)
BsoBI 633 CTCGAG C^[CT]CG[GA]G
BsrDI 141 GCAATG GCAATG(2/0)
BsrFI 712 ACCGGT [GA]^CCGG[CT]
BsrI 341 CCAGT ACTGG(1/-1)
Cac8I 843 GCCTGC GC[ACGT]^[ACGT]GC
CfoI 522 GCGC GCG^C
Cfr10I 712 ACCGGT [GA]^CCGG[CT]
DraIII 109 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 58 TGGCCA [CT]^GGCC[GA]
EcoT14I 242 CCAAGG C^C[AT][AT]GG
FbaI 362 TGATCA T^GATCA
FspI 521 TGCGCA TGC^GCA
HgaI 610 GACGC GACGC(5/10)
HhaI 522 GCGC GCG^C
HinP1I 522 GCGC G^CGC
MaeII 504 ACGT A^CGT
MluNI 58 TGGCCA TGG^CCA
MscI 58 TGGCCA TGG^CCA
MseI 197 TTAA T^TAA
MwoI 449 GCTCCCGGAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PaeR7I 633 CTCGAG C^TCGAG
PflFI 435 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 712 ACCGGT A^CCGGT
PleI 71 GAGTC GAGTC(4/5)
RsaI 471 GTAC GT^AC
SacI 456 GAGCTC GAGCT^C
SbfI 844 CCTGCAGG CCTGCA^GG
SexAI 110 ACCTGGT A^CC[AT]GGT
SmlI 633 CTCGAG C^T[CT][GA]AG
Sse8387I 844 CCTGCAGG CCTGCA^GG
StyI 242 CCAAGG C^C[AT][AT]GG
TaqI 634 TCGA T^CGA
TfiI 874 GATTC G^A[AT]TC
Tru9I 197 TTAA T^TAA
Tth111I 435 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 634 TCGA T^CGA
XcmI 341 CCAGTGACCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 633 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflIII 467 ACATGT A^C[GA][CT]GT
732 ACATGT
ApaI 405 GGGCCC GGGCC^C
561 GGGCCC
Asp700I 80 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
665 GAAACCCTTC
BcnI 403 CCGGG CC^[GC]GG
452 CCCGG
BspLU11I 467 ACATGT A^CATGT
732 ACATGT
BstEII 106 GGTCACC G^GT[ACGT]ACC
715 GGTAACC
BstPI 106 GGTCACC G^GT[ACGT]ACC
715 GGTAACC
DdeI 393 CTCAG C^T[ACGT]AG
750 CTCAG
DpnII 363 GATC ^GATC
640 GATC
DsaI 410 CCACGG C^C[GA][CT]GG
573 CCACGG
EarI 781 CTCTTC CTCTTC(1/4)
124 GAAGAG
EcoO65I 106 GGTCACC G^GT[ACGT]ACC
715 GGTAACC
HinfI 71 GAGTC G^A[ACGT]TC
874 GATTC
Ksp632I 781 CTCTTC CTCTTC(1/4)
124 GAAGAG
MboI 363 GATC ^GATC
640 GATC
NciI 403 CCGGG CC^[GC]GG
452 CCCGG
NdeII 363 GATC ^GATC
640 GATC
NspI 467 ACATGT [GA]CATG^[CT]
732 ACATGT
PpuMI 580 GGGACCT [GA]G^G[AT]CC[CT]
745 GGGACCT
Sau3AI 363 GATC ^GATC
640 GATC
XmnI 80 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
665 GAAACCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AluI AspHI AvaII BanII BbvI
BmyI BsaJI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BssKI BstF5I BstNI
Cfr13I DraII EcoO109I EcoRII Fnu4HI
FokI HaeIII HapII HpaII HphI
ItaI MaeIII MboII MnlI MslI
MspI MvaI NlaIII NlaIV PstI
Sau96I ScrFI SfaNI SfcI TseI
Tsp45I TspRI