Restriction map for
pFN21AE2210
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# NO CUT ENZYMES ( 134 enzymes) #
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AatII Acc65I AccI AclI AcyI
AflII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspHI AspI
AvaI AviII AvrII BamHI BanI
BbeI BbrPI BbvCI BcgI BciVI
BfrI BglI BlnI BsaAI BsaBI
BsaHI BsiEI BsiHKAI BsiWI BsmBI
BsoBI Bsp1407I BspDI BsrBI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstZ17I Bsu36I CfoI
Cfr10I ClaI CpoI DraI DraIII
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoT14I
FauI FseI FspI HaeII HhaI
Hin1I HinP1I HindIII HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MspA1I MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI StyI SwaI Tth111I Van91I
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 32 enzymes) #
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AccII 2317 CGCG CG^CG
AccIII 368 TCCGGA T^CCGGA
AflIII 1445 ACATGT A^C[GA][CT]GT
ApaI 209 GGGCCC GGGCC^C
BclI 1080 TGATCA T^GATCA
BcnI 1031 CCCGG CC^[GC]GG
BpmI 1519 CTGGAG CTGGAG(16/14)
BsaI 2042 GAGACC GGTCTC(1/5)
BsaWI 368 TCCGGA [AT]^CCGG[AT]
BseAI 368 TCCGGA T^CCGGA
BsmFI 2631 GTCCC GGGAC(10/14)
BspEI 368 TCCGGA T^CCGGA
BspHI 1077 TCATGA T^CATGA
BspLU11I 1445 ACATGT A^CATGT
BsrDI 2622 CATTGC GCAATG(2/0)
BstAPI 2496 GCACTCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 2317 CGCG CG^CG
DrdI 1037 GACTGGCCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 1080 TGATCA T^GATCA
HgaI 2034 GCGTC GACGC(5/10)
HincII 640 GTCAAC GT[CT]^[GA]AC
HindII 640 GTCAAC GT[CT]^[GA]AC
MroI 368 TCCGGA T^CCGGA
MvnI 2317 CGCG CG^CG
NciI 1031 CCCGG CC^[GC]GG
PpuMI 1613 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 1077 TCATGA T^CATGA
SapI 952 GCTCTTC GCTCTTC(1/4)
ScaI 1852 AGTACT AGT^ACT
SphI 15 GCATGC GCATG^C
SspI 1710 AATATT AAT^ATT
StuI 905 AGGCCT AGG^CCT
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# TWO CUT ENZYMES ( 30 enzymes) #
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AciI 1692 CCGC CCGC(-3/-1)
553 GCGG
Asp700I 415 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2146 GAAAACTTTC
BalI 845 TGGCCA TGG^CCA
1040 TGGCCA
BanII 209 GGGCCC G[GA]GC[CT]^C
1522 GAGCCC
BbsI 2118 GAAGAC GAAGAC(2/6)
1331 GTCTTC
BlpI 60 GCTGAGC GC^T[ACGT]AGC
2029 GCTGAGC
BmyI 209 GGGCCC G[AGT]GC[ACT]^C
1522 GAGCCC
Bpu1102I 60 GCTGAGC GC^T[ACGT]AGC
2029 GCTGAGC
BpuAI 2118 GAAGAC GAAGAC(2/6)
1331 GTCTTC
BseRI 2075 GAGGAG GAGGAG(10/8)
2338 GAGGAG
BsmI 324 GAATGC GAATGC(1/-1)
549 GAATGC
Bsp1286I 209 GGGCCC G[AGT]GC[ACT]^C
1522 GAGCCC
BspMI 144 GCAGGT ACCTGC(4/8)
588 GCAGGT
BstXI 1166 CCAAGCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2126 CCAGGGATGTGG
CelII 60 GCTGAGC GC^T[ACGT]AGC
2029 GCTGAGC
EaeI 845 TGGCCA [CT]^GGCC[GA]
1040 TGGCCA
EarI 953 CTCTTC CTCTTC(1/4)
2470 GAAGAG
EcoRI 1964 GAATTC G^AATTC
2584 GAATTC
EcoRV 362 GATATC GAT^ATC
508 GATATC
HapII 369 CCGG C^CGG
1032 CCGG
HpaII 369 CCGG C^CGG
1032 CCGG
Ksp632I 953 CTCTTC CTCTTC(1/4)
2470 GAAGAG
MluNI 845 TGGCCA TGG^CCA
1040 TGGCCA
MscI 845 TGGCCA TGG^CCA
1040 TGGCCA
MspI 369 CCGG C^CGG
1032 CCGG
NspI 15 GCATGC [GA]CATG^[CT]
1445 ACATGT
PleI 1092 GACTC GAGTC(4/5)
1803 GAGTC
PstI 586 CTGCAG CTGCA^G
934 CTGCAG
XbaI 2196 TCTAGA T^CTAGA
2427 TCTAGA
XmnI 415 GAATCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2146 GAAAACTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 62 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
AvaII BbvI BfaI BglII BsaJI
BsgI BsiYI BslI BsmAI BsrI
BssKI BstF5I BstNI BstYI Cac8I
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII EcoT22I Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MvaI MwoI NdeII NlaIII
NlaIV NsiI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII