Restriction map for
pFN21AE2238
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# NO CUT ENZYMES ( 164 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AlwNI AocI Aor51HI
ApaLI AscI Asp718I AspEI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbvCI BciVI BclI BcnI
BfrI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiWI
BsmBI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I ClaI CpoI DpnII DraI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HpaI HpaII KasI
KpnI KspI MamI MboI MfeI
MflI MluI MluNI MroI MscI
MspA1I MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NspI
NspV PacI PaeR7I PflMI PinAI
PmeI PshAI Psp1406I PvuI PvuII
RcaI RsrII SacII SalI SapI
Sau3AI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI SspBI SspI SwaI
TaqI TthHB8I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AccI 771 GTCTAC GT^[AC][GT]AC
AcsI 653 AAATTC [GA]^AATT[CT]
AfaI 196 GTAC GT^AC
ApaI 789 GGGCCC GGGCC^C
ApoI 653 AAATTC [GA]^AATT[CT]
AseI 934 ATTAAT AT^TAAT
AsnI 934 ATTAAT AT^TAAT
Asp700I 752 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 442 GAGCTC G[AT]GC[AT]^C
AvaII 549 GGACC G^G[AT]CC
BbrPI 226 CACGTG CAC^GTG
BbvI 68 GCAGC GCAGC(8/12)
BcgI 704 GCATAGTCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpmI 124 CTGGAG CTGGAG(16/14)
BsaAI 226 CACGTG [CT]AC^GT[GA]
BsiHKAI 442 GAGCTC G[AT]GC[AT]^C
BsmFI 548 GGGAC GGGAC(10/14)
BsmI 594 GAATGC GAATGC(1/-1)
BspMI 580 ACCTGC ACCTGC(4/8)
BstAPI 589 GCAGAGAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 468 GGATG GGATG(2/0)
EcoRV 330 GATATC GAT^ATC
EcoT14I 503 CCTTGG C^C[AT][AT]GG
EcoT22I 702 ATGCAT ATGCA^T
FauI 793 CCCGC CCCGC(4/6)
Fnu4HI 68 GCAGC GC^[ACGT]GC
FokI 468 GGATG GGATG(9/13)
HincII 148 GTCAAC GT[CT]^[GA]AC
HindII 148 GTCAAC GT[CT]^[GA]AC
HindIII 634 AAGCTT A^AGCTT
ItaI 68 GCAGC GC^[ACGT]GC
MaeII 227 ACGT A^CGT
MslI 488 CATTCAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NlaIII 202 CATG CATG^
NsiI 702 ATGCAT ATGCA^T
PmaCI 226 CACGTG CAC^GTG
PmlI 226 CACGTG CAC^GTG
PpuMI 548 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 934 ATTAAT AT^TAAT
PstI 134 CTGCAG CTGCA^G
RsaI 196 GTAC GT^AC
SacI 442 GAGCTC GAGCT^C
SbfI 133 CCTGCAGG CCTGCA^GG
SfaNI 469 GATGC GCATC(5/9)
SfcI 134 CTGCAG C^T[GA][CT]AG
Sse8387I 133 CCTGCAGG CCTGCA^GG
StuI 500 AGGCCT AGG^CCT
StyI 503 CCTTGG C^C[AT][AT]GG
TseI 68 GCAGC G^C[AT]GC
XmnI 752 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 12 enzymes) #
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AciI 794 CCGC CCGC(-3/-1)
803 CCGC
AspI 478 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
985 GACAATGTC
BfaI 814 CTAG C^TAG
899 CTAG
EarI 116 CTCTTC CTCTTC(1/4)
752 GAAGAG
HphI 87 GGTGA GGTGA(8/7)
263 TCACC
Ksp632I 116 CTCTTC CTCTTC(1/4)
752 GAAGAG
MaeI 814 CTAG C^TAG
899 CTAG
MwoI 589 GCAGAGAATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
796 GCAGAGTCCGC
PflFI 478 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
985 GACAATGTC
PleI 106 GAGTC GAGTC(4/5)
799 GAGTC
Tsp45I 229 GTGAC ^GT[GC]AC
862 GTCAC
Tth111I 478 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
985 GACAATGTC
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# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AluI BanII BbsI BmyI BpuAI
BsaJI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstNI Cfr13I DdeI
DraII EcoO109I EcoRII HaeIII HinfI
MaeIII MboII MnlI MseI MvaI
NlaIV Sau96I ScrFI TfiI Tru9I
Tsp509I TspRI