##################################
# NO CUT ENZYMES (  214 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AciI        AclI        AcsI        AcyI        AflII       
AflIII      AgeI        AhdI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
ApoI        AscI        AseI        AsnI        Asp718I     
AspEI       AspHI       AspI        AvaI        AvaII       
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbvCI       BcgI        
BciVI       BclI        BcnI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        Bpu1102I    
BsaBI       BsaHI       BsaI        BsaWI       BseAI       
BsgI        BsiEI       BsiHKAI     BsiWI       BsiYI       
BslI        BsmAI       BsmBI       BsmFI       BsmI        
BsoBI       Bsp1286I    Bsp1407I    BspDI       BspEI       
BspHI       BspLU11I    BspMI       BsrBI       BsrDI       
BsrFI       BsrGI       BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      Cac8I       
CelII       CfoI        Cfr10I      Cfr13I      ClaI        
CpoI        DpnII       DraI        DraII       DraIII      
DrdI        DsaI        EaeI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      Eco81I      EcoNI       
EcoO109I    EcoO65I     EcoRI       EcoRV       EcoT22I     
FauI        FbaI        FseI        FspI        HaeII       
HaeIII      HapII       HgaI        HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
HpaII       KasI        KpnI        KspI        MamI        
MboI        MfeI        MflI        MluI        MluNI       
MroI        MscI        MspI        MunI        MvnI        
MwoI        NaeI        NarI        NciI        NcoI        
NdeI        NdeII       NgoMIV      NheI        NlaIV       
NotI        NruI        NsiI        NspI        NspV        
PacI        PaeR7I      PflFI       PflMI       PinAI       
PmaCI       PmeI        PmlI        PpuMI       PshAI       
PshBI       Psp1406I    PstI        PvuI        RcaI        
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      Sau96I      SbfI        ScaI        SexAI       
SfaNI       SfcI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
TaqI        TfiI        Tsp45I      Tsp509I     TspRI       
Tth111I     TthHB8I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   38 enzymes) #
###################################

AfaI          130  GTAC                 GT^AC
Asp700I       239  GAAGAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BbsI          379  GAAGAC               GAAGAC(2/6)
BbvI           88  GCTGC                GCAGC(8/12)
BfaI          331  CTAG                 C^TAG
BpmI          270  CTCCAG               CTGGAG(16/14)
BpuAI         379  GAAGAC               GAAGAC(2/6)
BsaAI         401  TACGTG               [CT]AC^GT[GA]
BsaJI         353  CCAAGG               C^C[ACGT][ACGT]GG
BseRI         139  GAGGAG               GAGGAG(10/8)
BssKI         123  CCTGG                ^CC[ACGT]GG
BstNI         123  CCTGG                CC^[AT]GG
DdeI           52  CTGAG                C^T[ACGT]AG
EarI          239  GAAGAG               CTCTTC(1/4)
EcoRII        123  CCTGG                ^CC[AT]GG
EcoT14I       353  CCAAGG               C^C[AT][AT]GG
Fnu4HI         88  GCTGC                GC^[ACGT]GC
HinfI           7  GAGTC                G^A[ACGT]TC
HphI          157  TCACC                GGTGA(8/7)
ItaI           88  GCTGC                GC^[ACGT]GC
Ksp632I       239  GAAGAG               CTCTTC(1/4)
MaeI          331  CTAG                 C^TAG
MaeII         402  ACGT                 A^CGT
MaeIII        399  GTTAC                ^GT[ACGT]AC
MseI          170  TTAA                 T^TAA
MslI          229  CATGATGATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I        341  CAGCTG               C[AC]G^C[GT]G
MvaI          123  CCTGG                CC^[AT]GG
NlaIII        229  CATG                 CATG^
PleI            7  GAGTC                GAGTC(4/5)
PvuII         341  CAGCTG               CAG^CTG
RsaI          130  GTAC                 GT^AC
ScrFI         123  CCTGG                CC^[ACGT]GG
SmlI          145  CTTGAG               C^T[CT][GA]AG
StyI          353  CCAAGG               C^C[AT][AT]GG
Tru9I         170  TTAA                 T^TAA
TseI           88  GCTGC                G^C[AT]GC
XmnI          239  GAAGAGTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (    3 enzymes) #
###################################

BsrI          132  ACTGG                ACTGG(1/-1)
              272  CCAGT               
BstF5I        135  GGATG                GGATG(2/0)
              283  GGATG               
FokI          135  GGATG                GGATG(9/13)
              283  GGATG               


#########################################
# 3 OR MORE CUT ENZYMES (    3 enzymes) #
#########################################

AluI        MboII       MnlI