Restriction map for
pFN21AE1964
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# NO CUT ENZYMES ( 114 enzymes) #
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AatII Acc65I AccI AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AlwI ApaLI ApoI AseI AsnI
Asp700I Asp718I AspEI AspI AvrII
BamHI BbrPI BbsI BbvCI BciVI
BclI BfrI BglII BlnI BpuAI
BsaAI BsaBI BsaI BsiWI BsmI
Bsp1407I BspDI BspHI BspLU11I BsrDI
BsrGI Bst1107I BstAPI BstBI BstYI
BstZ17I ClaI CpoI DpnII DraI
EagI Eam1105I EclXI Eco52I EcoRI
EcoRV EcoT22I FbaI FseI HindIII
HinfI HpaI KpnI MamI MboI
MfeI MflI MluI MseI MslI
MunI NdeI NdeII NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI Sau3AI
SbfI ScaI SfuI SgrAI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI TfiI Tru9I Tsp509I Tth111I
XbaI XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccIII 644 TCCGGA T^CCGGA
AfaI 1080 GTAC GT^AC
AocI 1417 CCTGAGG CC^T[ACGT]AGG
AscI 86 GGCGCGCC GG^CGCGCC
AviII 626 TGCGCA TGC^GCA
BalI 296 TGGCCA TGG^CCA
BbeI 478 GGCGCC GGCGC^C
BcgI 735 CGAGGCGGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 1282 CTAG C^TAG
BseAI 644 TCCGGA T^CCGGA
BsiEI 573 CGACCG CG[GA][CT]^CG
BsmBI 895 GAGACG CGTCTC(1/5)
BspEI 644 TCCGGA T^CCGGA
BsrBI 61 GAGCGG CCGCTC(-3/-3)
BssSI 850 CTCGTG CACGAG(-5/-1)
BstEII 1470 GGTGACC G^GT[ACGT]ACC
BstPI 1470 GGTGACC G^GT[ACGT]ACC
Bsu36I 1417 CCTGAGG CC^T[ACGT]AGG
DraIII 451 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1285 GACCGGAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 202 CTCTTC CTCTTC(1/4)
Eco81I 1417 CCTGAGG CC^T[ACGT]AGG
EcoO65I 1470 GGTGACC G^GT[ACGT]ACC
FspI 626 TGCGCA TGC^GCA
HincII 496 GTCAAC GT[CT]^[GA]AC
HindII 496 GTCAAC GT[CT]^[GA]AC
KasI 478 GGCGCC G^GCGCC
Ksp632I 202 CTCTTC CTCTTC(1/4)
MaeI 1282 CTAG C^TAG
MaeII 710 ACGT A^CGT
MluNI 296 TGGCCA TGG^CCA
MroI 644 TCCGGA T^CCGGA
MscI 296 TGGCCA TGG^CCA
NarI 478 GGCGCC GG^CGCC
RsaI 1080 GTAC GT^AC
SacI 1180 GAGCTC GAGCT^C
SexAI 452 ACCTGGT A^CC[AT]GGT
SfiI 603 GGCCGCCCGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 478 GGCGCC GGC^GCC
SmaI 608 CCCGGG CCC^GGG
SmlI 1522 CTCAAG C^T[CT][GA]AG
TaqI 599 TCGA T^CGA
TthHB8I 599 TCGA T^CGA
XcmI 1479 CCAGCCTGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 608 CCCGGG C^CCGGG
XmaI 608 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AlwNI 364 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
541 CAGACGCTG
Aor51HI 775 AGCGCT AGC^GCT
992 AGCGCT
ApaI 110 GGGCCC GGGCC^C
1423 GGGCCC
BanI 478 GGCGCC G^G[CT][GA]CC
831 GGTGCC
BlpI 1305 GCTGAGC GC^T[ACGT]AGC
1444 GCTGAGC
Bpu1102I 1305 GCTGAGC GC^T[ACGT]AGC
1444 GCTGAGC
BsaWI 644 TCCGGA [AT]^CCGG[AT]
1286 ACCGGA
BsrFI 650 GCCGGC [GA]^CCGG[CT]
1024 GCCGGC
BssHII 87 GCGCGC G^CGCGC
975 GCGCGC
BstF5I 642 CATCC GGATG(2/0)
47 GGATG
CelII 1305 GCTGAGC GC^T[ACGT]AGC
1444 GCTGAGC
Cfr10I 650 GCCGGC [GA]^CCGG[CT]
1024 GCCGGC
Eco47III 775 AGCGCT AGC^GCT
992 AGCGCT
EcoNI 189 CCTGAAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
222 CCTTCTGGAGG
FokI 642 CATCC GGATG(9/13)
47 GGATG
KspI 812 CCGCGG CCGC^GG
1032 CCGCGG
NaeI 650 GCCGGC GCC^GGC
1024 GCCGGC
NcoI 35 CCATGG C^CATGG
566 CCATGG
NgoMIV 650 GCCGGC G^CCGGC
1024 GCCGGC
PstI 655 CTGCAG CTGCA^G
1402 CTGCAG
PvuII 1303 CAGCTG CAG^CTG
1442 CAGCTG
SacII 812 CCGCGG CCGC^GG
1032 CCGCGG
SfcI 655 CTGCAG C^T[GA][CT]AG
1402 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AcyI AluI AspHI
AvaI AvaII BanII BbvI BcnI
BglI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstNI BstUI BstXI
Cac8I CfoI Cfr13I DdeI DraII
DsaI EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HpaII
HphI ItaI MaeIII MboII MnlI
MspA1I MspI MvaI MvnI MwoI
NciI NlaIII NlaIV NspI PflMI
PpuMI Sau96I ScrFI SfaNI SphI
StyI TseI Tsp45I TspRI Van91I