Restriction map for
pFN21AE1879
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# NO CUT ENZYMES ( 152 enzymes) #
##################################
AatII AccII AccIII AciI AclI
AcsI AcyI AgeI AhdI Alw44I
AlwNI Aor51HI ApaI ApaLI ApoI
AscI Asp700I AspEI AspI AvaI
AviII AvrII BamHI BanII BbeI
BbrPI BbvCI BcgI BciVI BclI
BcnI BglI BlnI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmAI BsmBI BsoBI
Bsp1407I BspDI BspEI BspMI BsrBI
BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstUI CfoI Cfr10I ClaI
CpoI DraII DraIII DrdI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO109I EcoRI EcoT14I EcoT22I
FauI FbaI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaI HpaII KasI KspI
MamI MfeI MluI MroI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI Psp1406I
PstI PvuI PvuII RsrII SacI
SacII SalI SbfI SexAI SfcI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SspI
StuI StyI SwaI TaqI Tth111I
TthHB8I Van91I XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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Acc65I 984 GGTACC G^GTACC
AccI 463 GTATAC GT^[AC][GT]AC
AflII 669 CTTAAG C^TTAAG
AflIII 347 ACATGT A^C[GA][CT]GT
AlwI 194 GGATC GGATC(4/5)
AocI 938 CCTCAGG CC^T[ACGT]AGG
AseI 289 ATTAAT AT^TAAT
AsnI 289 ATTAAT AT^TAAT
Asp718I 984 GGTACC G^GTACC
AspHI 1185 GAGCAC G[AT]GC[AT]^C
AvaII 425 GGTCC G^G[AT]CC
BalI 172 TGGCCA TGG^CCA
BanI 984 GGTACC G^G[CT][GA]CC
BfrI 669 CTTAAG C^TTAAG
BglII 837 AGATCT A^GATCT
BmyI 1185 GAGCAC G[AGT]GC[ACT]^C
BsaJI 107 CCCAGG C^C[ACGT][ACGT]GG
BseRI 130 GAGGAG GAGGAG(10/8)
BsiHKAI 1185 GAGCAC G[AT]GC[AT]^C
BsiYI 89 CCAAGAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 89 CCAAGAAGAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmI 399 GCATTC GAATGC(1/-1)
Bsp1286I 1185 GAGCAC G[AGT]GC[ACT]^C
BspHI 375 TCATGA T^CATGA
BspLU11I 347 ACATGT A^CATGT
Bst1107I 463 GTATAC GTA^TAC
BstEII 135 GGTCACC G^GT[ACGT]ACC
BstPI 135 GGTCACC G^GT[ACGT]ACC
BstXI 917 CCATTGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 837 AGATCT [GA]^GATC[CT]
BstZ17I 463 GTATAC GTA^TAC
Bsu36I 938 CCTCAGG CC^T[ACGT]AGG
Cac8I 649 GCTTGC GC[ACGT]^[ACGT]GC
Cfr13I 425 GGTCC G^G[ACGT]CC
EaeI 172 TGGCCA [CT]^GGCC[GA]
Eco81I 938 CCTCAGG CC^T[ACGT]AGG
EcoO65I 135 GGTCACC G^GT[ACGT]ACC
EcoRV 664 GATATC GAT^ATC
HincII 522 GTTGAC GT[CT]^[GA]AC
HindII 522 GTTGAC GT[CT]^[GA]AC
KpnI 984 GGTACC GGTAC^C
MaeII 635 ACGT A^CGT
MflI 837 AGATCT [GA]^GATC[CT]
MluNI 172 TGGCCA TGG^CCA
MscI 172 TGGCCA TGG^CCA
NspI 347 ACATGT [GA]CATG^[CT]
PshAI 1139 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 289 ATTAAT AT^TAAT
RcaI 375 TCATGA T^CATGA
SapI 1182 GAAGAGC GCTCTTC(1/4)
Sau96I 425 GGTCC G^G[ACGT]CC
ScaI 1009 AGTACT AGT^ACT
SpeI 1012 ACTAGT A^CTAGT
XbaI 435 TCTAGA T^CTAGA
XhoII 837 AGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
BbsI 1029 GAAGAC GAAGAC(2/6)
1054 GAAGAC
BlpI 28 GCTAAGC GC^T[ACGT]AGC
826 GCTCAGC
BpmI 252 CTCCAG CTGGAG(16/14)
1099 CTGGAG
Bpu1102I 28 GCTAAGC GC^T[ACGT]AGC
826 GCTCAGC
BpuAI 1029 GAAGAC GAAGAC(2/6)
1054 GAAGAC
BsmFI 953 GGGAC GGGAC(10/14)
1137 GGGAC
BsrDI 582 CATTGC GCAATG(2/0)
1157 GCAATG
CelII 28 GCTAAGC GC^T[ACGT]AGC
826 GCTCAGC
DpnII 195 GATC ^GATC
838 GATC
DraI 274 TTTAAA TTT^AAA
699 TTTAAA
HaeIII 87 GGCC GG^CC
173 GGCC
MboI 195 GATC ^GATC
838 GATC
MwoI 36 GCAGCCCCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1157 GCAATGGAGGC
NdeII 195 GATC ^GATC
838 GATC
NlaIII 348 CATG CATG^
376 CATG
NlaIV 411 GGAACC GG[ACGT]^[ACGT]CC
984 GGTACC
Sau3AI 195 GATC ^GATC
838 GATC
SfaNI 760 GATGC GCATC(5/9)
772 GATGC
SmlI 595 CTTGAG C^T[CT][GA]AG
669 CTTAAG
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AfaI AluI BbvI BfaI BsrI
BssKI BstF5I BstNI DdeI EarI
EcoRII Fnu4HI FokI HinfI HphI
ItaI Ksp632I MaeI MaeIII MboII
MnlI MseI MslI MvaI RsaI
ScrFI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI