Restriction map for
pFN21AB5889
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# NO CUT ENZYMES ( 102 enzymes) #
##################################
Acc65I AclI AcsI AflII AgeI
AhdI AlwNI ApoI AscI AseI
AsnI Asp718I AspEI AspI AvrII
BalI BamHI BbvCI BciVI BclI
BfaI BfrI BglII BlnI BsaBI
BsaI BsiWI BsmI Bsp1407I BspDI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstBI BstXI BstYI BstZ17I ClaI
CpoI DraI DrdI Eam1105I EarI
EcoNI EcoRI EcoRV EcoT22I FbaI
HincII HindII HindIII HpaI KpnI
Ksp632I MaeI MamI MfeI MflI
MluI MluNI MscI MseI MunI
NdeI NheI NruI NsiI NspV
PacI PaeR7I PflFI PflMI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RsrII SalI SapI ScaI SexAI
SfuI SmlI SnaBI SpeI SphI
SspBI SspI SwaI TfiI Tru9I
Tsp509I Tth111I Van91I XbaI XcmI
XhoI XhoII
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 868 GACGTC GACGT^C
AccI 871 GTCTAC GT^[AC][GT]AC
AfaI 1156 GTAC GT^AC
AflIII 1184 ACGTGT A^C[GA][CT]GT
AocI 100 CCTCAGG CC^T[ACGT]AGG
Aor51HI 941 AGCGCT AGC^GCT
ApaI 562 GGGCCC GGGCC^C
Asp700I 84 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 1301 TGCGCA TGC^GCA
BbsI 546 GAAGAC GAAGAC(2/6)
BcgI 683 CGACCATGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 546 GAAGAC GAAGAC(2/6)
BseRI 1165 GAGGAG GAGGAG(10/8)
BsgI 1368 GTGCAG GTGCAG(16/14)
BsmAI 1451 GAGAC GTCTC(1/5)
BsmBI 1451 GAGACG CGTCTC(1/5)
BspHI 80 TCATGA T^CATGA
BsrBI 723 GAGCGG CCGCTC(-3/-3)
BssSI 1162 CACGAG CACGAG(-5/-1)
BstEII 795 GGTGACC G^GT[ACGT]ACC
BstPI 795 GGTGACC G^GT[ACGT]ACC
Bsu36I 100 CCTCAGG CC^T[ACGT]AGG
DraIII 1309 CACGCGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 941 AGCGCT AGC^GCT
Eco81I 100 CCTCAGG CC^T[ACGT]AGG
EcoO65I 795 GGTGACC G^GT[ACGT]ACC
FspI 1301 TGCGCA TGC^GCA
NcoI 686 CCATGG C^CATGG
NotI 298 GCGGCCGC GC^GGCCGC
NspI 1409 ACATGC [GA]CATG^[CT]
PstI 124 CTGCAG CTGCA^G
RcaI 80 TCATGA T^CATGA
RsaI 1156 GTAC GT^AC
SbfI 123 CCTGCAGG CCTGCA^GG
SfcI 124 CTGCAG C^T[GA][CT]AG
SfiI 222 GGCCCCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 123 CCTGCAGG CCTGCA^GG
StuI 857 AGGCCT AGG^CCT
TaqI 505 TCGA T^CGA
TthHB8I 505 TCGA T^CGA
XmnI 84 GAACTCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccIII 614 TCCGGA T^CCGGA
824 TCCGGA
AlwI 1047 GGATC GGATC(4/5)
1134 GGATC
BbrPI 973 CACGTG CAC^GTG
1183 CACGTG
BsaAI 973 CACGTG [CT]AC^GT[GA]
1183 CACGTG
BsaWI 614 TCCGGA [AT]^CCGG[AT]
824 TCCGGA
BseAI 614 TCCGGA T^CCGGA
824 TCCGGA
BspEI 614 TCCGGA T^CCGGA
824 TCCGGA
BstAPI 1352 GCATCCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1361 GCAAGCTGTGC
DpnII 1048 GATC ^GATC
1135 GATC
FseI 648 GGCCGGCC GGCCGG^CC
1401 GGCCGGCC
HgaI 1453 GACGC GACGC(5/10)
529 GCGTC
MboI 1048 GATC ^GATC
1135 GATC
MroI 614 TCCGGA T^CCGGA
824 TCCGGA
NdeII 1048 GATC ^GATC
1135 GATC
PmaCI 973 CACGTG CAC^GTG
1183 CACGTG
PmlI 973 CACGTG CAC^GTG
1183 CACGTG
PpuMI 656 GGGTCCC [GA]G^G[AT]CC[CT]
1245 GGGTCCT
PvuII 718 CAGCTG CAG^CTG
1396 CAGCTG
Sau3AI 1048 GATC ^GATC
1135 GATC
SgrAI 197 CGCCGGCG C[GA]^CCGG[CT]G
783 CGCCGGTG
SmaI 62 CCCGGG CCC^GGG
1446 CCCGGG
XmaCI 62 CCCGGG C^CCGGG
1446 CCCGGG
XmaI 62 CCCGGG C^CCGGG
1446 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcyI AluI Alw44I
ApaLI AspHI AvaI AvaII BanI
BanII BbeI BbvI BcnI BglI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaJI BsiEI BsiHKAI BsiYI BslI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DraII DsaI EaeI EagI
EclXI Eco52I EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI KspI
MaeII MaeIII MboII MnlI MslI
MspA1I MspI MvaI MvnI MwoI
NaeI NarI NciI NgoMIV NlaIII
NlaIV PleI SacI SacII Sau96I
ScrFI SfaNI SfoI StyI TseI
Tsp45I TspRI