Restriction map for
pFN21AE1951
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# NO CUT ENZYMES ( 110 enzymes) #
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Acc65I AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BamHI BbrPI BbsI BcgI
BciVI BclI BfrI BlnI BpuAI
BsaAI BsaBI BsaWI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BsrGI BssSI Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraI DraIII DrdI Eam1105I Eco47III
EcoO65I EcoRI EcoRV FbaI FseI
FspI HincII HindII HindIII HpaI
KpnI MamI MfeI MluI MroI
MseI MunI NaeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI ScaI SfiI
SfuI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tsp509I Tth111I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 41 enzymes) #
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AatII 1494 GACGTC GACGT^C
AocI 1441 CCTCAGG CC^T[ACGT]AGG
ApaI 84 GGGCCC GGGCC^C
AspHI 855 GAGCTC G[AT]GC[AT]^C
BalI 290 TGGCCA TGG^CCA
BfaI 1240 CTAG C^TAG
BglI 323 GCCAAAAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 662 AGATCT A^GATCT
BlpI 634 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 634 GCTGAGC GC^T[ACGT]AGC
BsgI 472 CTGCAC GTGCAG(16/14)
BsiEI 41 CGGCCG CG[GA][CT]^CG
BsiHKAI 855 GAGCTC G[AT]GC[AT]^C
BsrDI 603 CATTGC GCAATG(2/0)
BstAPI 1378 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1441 CCTCAGG CC^T[ACGT]AGG
CelII 634 GCTGAGC GC^T[ACGT]AGC
EagI 41 CGGCCG C^GGCCG
EarI 6 GAAGAG CTCTTC(1/4)
EclXI 41 CGGCCG C^GGCCG
Eco52I 41 CGGCCG C^GGCCG
Eco81I 1441 CCTCAGG CC^T[ACGT]AGG
EcoNI 75 CCTGGCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 91 GACGC GACGC(5/10)
Ksp632I 6 GAAGAG CTCTTC(1/4)
KspI 38 CCGCGG CCGC^GG
MaeI 1240 CTAG C^TAG
MluNI 290 TGGCCA TGG^CCA
MscI 290 TGGCCA TGG^CCA
NcoI 1361 CCATGG C^CATGG
NdeI 625 CATATG CA^TATG
PflMI 1044 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SacI 855 GAGCTC GAGCT^C
SacII 38 CCGCGG CCGC^GG
SapI 6 GAAGAGC GCTCTTC(1/4)
SexAI 1191 ACCTGGT A^CC[AT]GGT
SgrAI 758 CGCCGGTG C[GA]^CCGG[CT]G
TfiI 309 GATTC G^A[AT]TC
TspRI 1138 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 1044 CCAGGAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1169 CCAAGGCTATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AflIII 425 ACATGT A^C[GA][CT]GT
878 ACATGT
AlwI 711 GATCC GGATC(4/5)
729 GATCC
AlwNI 364 CAGTACCTG CAG[ACGT][ACGT][ACGT]^CTG
1187 CAGAACCTG
BbeI 196 GGCGCC GGCGC^C
597 GGCGCC
BseRI 802 CTCCTC GAGGAG(10/8)
574 GAGGAG
BspLU11I 425 ACATGT A^CATGT
878 ACATGT
BspMI 470 ACCTGC ACCTGC(4/8)
677 ACCTGC
BstYI 662 AGATCT [GA]^GATC[CT]
728 AGATCC
EcoT22I 622 ATGCAT ATGCA^T
1385 ATGCAT
KasI 196 GGCGCC G^GCGCC
597 GGCGCC
MaeII 455 ACGT A^CGT
1495 ACGT
MflI 662 AGATCT [GA]^GATC[CT]
728 AGATCC
MslI 621 CATGCATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1362 CATGGTGGTG
NarI 196 GGCGCC GG^CGCC
597 GGCGCC
NsiI 622 ATGCAT ATGCA^T
1385 ATGCAT
PpuMI 437 GGGACCC [GA]G^G[AT]CC[CT]
530 AGGACCT
PstI 1135 CTGCAG CTGCA^G
1376 CTGCAG
SfoI 196 GGCGCC GGC^GCC
597 GGCGCC
StuI 551 AGGCCT AGG^CCT
1031 AGGCCT
Tsp45I 467 GTGAC ^GT[GC]AC
1066 GTGAC
XhoII 662 AGATCT [GA]^GATC[CT]
728 AGATCC
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# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaI AvaII BanI BanII BbvCI
BbvI BcnI BmyI BpmI BsaHI
BsaI BsaJI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrBI BsrFI
BsrI BssHII BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeIII MboI MboII
MnlI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmaI
StyI TaqI TseI TthHB8I XmaCI
XmaI