Restriction map for
pFN21AB6361
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# NO CUT ENZYMES ( 153 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI Alw44I AlwI AlwNI AocI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspHI AspI
AviII AvrII BamHI BbeI BbrPI
BbvCI BcgI BclI BfaI BfrI
BglII BlnI BpmI BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsgI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspLU11I BspMI BsrBI
BsrDI BsrGI BsrI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstYI BstZ17I Bsu36I ClaI CpoI
DpnII DraI DraII DraIII DrdI
EarI Eco81I EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I MaeI MamI
MboI MfeI MflI MluI MroI
MseI MunI NarI NdeI NdeII
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI RsaI RsrII
SacI SalI SapI Sau3AI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SnaBI SpeI Sse8387I SspBI
SspI StuI SwaI TaqI TfiI
Tru9I Tsp509I Tth111I TthHB8I XbaI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AhdI 30 GACCCTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 361 AGCGCT AGC^GCT
AspEI 30 GACCCTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 147 CCCGGG C^[CT]CG[GA]G
BalI 413 TGGCCA TGG^CCA
BanI 223 GGCACC G^G[CT][GA]CC
BbsI 478 GAAGAC GAAGAC(2/6)
BciVI 294 GTATCC GTATCC(6/5)
BglI 433 GCCATGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 357 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 357 GCTGAGC GC^T[ACGT]AGC
BpuAI 478 GAAGAC GAAGAC(2/6)
BsaI 37 GGTCTC GGTCTC(1/5)
BsiEI 70 CGGCCG CG[GA][CT]^CG
BsoBI 147 CCCGGG C^[CT]CG[GA]G
BspHI 207 TCATGA T^CATGA
BssHII 16 GCGCGC G^CGCGC
BstF5I 266 CATCC GGATG(2/0)
CelII 357 GCTGAGC GC^T[ACGT]AGC
EagI 70 CGGCCG C^GGCCG
Eam1105I 30 GACCCTCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 70 CGGCCG C^GGCCG
Eco47III 361 AGCGCT AGC^GCT
Eco52I 70 CGGCCG C^GGCCG
EcoNI 178 CCTTCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 434 CCATGG C^C[AT][AT]GG
FokI 266 CATCC GGATG(9/13)
HgaI 81 GACGC GACGC(5/10)
HinfI 211 GAGTC G^A[ACGT]TC
KspI 7 CCGCGG CCGC^GG
MaeII 334 ACGT A^CGT
MaeIII 451 GTGAC ^GT[ACGT]AC
MboII 478 GAAGA GAAGA(8/7)
MluNI 413 TGGCCA TGG^CCA
MscI 413 TGGCCA TGG^CCA
MslI 315 CACCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 75 GCCGGC GCC^GGC
NcoI 434 CCATGG C^CATGG
NgoMIV 75 GCCGGC G^CCGGC
NspI 379 GCATGC [GA]CATG^[CT]
PflMI 245 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 211 GAGTC GAGTC(4/5)
PvuII 157 CAGCTG CAG^CTG
RcaI 207 TCATGA T^CATGA
SacII 7 CCGCGG CCGC^GG
SgrAI 74 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 147 CCCGGG CCC^GGG
SmlI 388 CTCAAG C^T[CT][GA]AG
SphI 379 GCATGC GCATG^C
StyI 434 CCATGG C^C[AT][AT]GG
Tsp45I 451 GTGAC ^GT[GC]AC
Van91I 245 CCAAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 416 CCAGCGTTGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 147 CCCGGG C^CCGGG
XmaI 147 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 12 enzymes) #
###################################
AvaII 29 GGACC G^G[AT]CC
461 GGACC
BanII 88 GAGCCC G[GA]GC[CT]^C
150 GGGCTC
BmyI 88 GAGCCC G[AGT]GC[ACT]^C
150 GGGCTC
BsmAI 38 GTCTC GTCTC(1/5)
369 GTCTC
BsmFI 460 GGGAC GGGAC(10/14)
145 GTCCC
Bsp1286I 88 GAGCCC G[AGT]GC[ACT]^C
150 GGGCTC
BsrFI 75 GCCGGC [GA]^CCGG[CT]
447 GCCGGT
Cfr10I 75 GCCGGC [GA]^CCGG[CT]
447 GCCGGT
DdeI 233 CTCAG C^T[ACGT]AG
358 CTGAG
HaeII 45 GGCGCT [GA]GCGC^[CT]
361 AGCGCT
SfaNI 162 GATGC GCATC(5/9)
265 GCATC
TspRI 318 CACTG CA[GC]TG[ACGT][ACGT]^
339 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AccII AciI AluI BbvI BcnI
BsaJI BsiYI BslI BssKI BstNI
BstUI Cac8I CfoI Cfr13I DsaI
EaeI EcoRII FauI Fnu4HI HaeIII
HapII HhaI HinP1I HpaII HphI
ItaI MnlI MspA1I MspI MvaI
MvnI MwoI NciI NlaIII NlaIV
Sau96I ScrFI TseI